Peculiarities of antioxidant protection system in the dynamics of acute respiratory distress syndrome development and at different methods of its correction in rats

Authors

DOI:

https://doi.org/10.15587/2519-4798.2017.105583

Keywords:

acute respiratory distress-syndrome, antioxidant system, correction, insufflation by oxygen, KD- 234, reamberin

Abstract

Taking into account the pathogenetic role of membrane destructing processes of the oxidative stress and hypoxia in ARDS development, it becomes obvious that it is necessary to use antihypoxants-antioxidants. During the last decade the large number of researchers searched for effective metabolic preparations to treat and prevent ARDS. This work is devoted to this important question of the modern medicine.

Aim of research – to determine the indices of the antioxidant protection system in the dynamics of an acute respiratory distress-syndrome development and at different correction methods in rats with a different tolerance to hypoxia.

Materials and methods. The study was carried out on 106 white non-linear male rats, kept on the standard ration of the vivarium of Ternopol state medical university, named after I.Y. Gorbachevsky. The experiment on the assessment of an effect of oxygen insufflation, “KD-234” and reamberin was carried out taking into account animals’ individual tolerance to hypoxia, determined by the method of V.Y. Berezovsky. For further studies were taken animals from the group of hypoxia middle tolerable rats HMT) with the survival time 240–360 s and low tolerable rats (HLT) with the survival time less than 180 s. Animals were divided in 5 groups: 1 – control group (n=12; HMT/HLT=6/6), 2 – ARDS modeling without correction, observations in 2 hours. (n=24: 12/12), 3 – ARDS modeling, correction by oxygen insufflation (n=24: 12/12), 4 – ARDS modeling “KD-234” correction (n=24: 12/12), 5 – ARDS modeling, reamberin correction (n=22: 11/11). Animal underwent ARDS modeling by G. Маtute-Bello method. For the correction in 4th studied group for used “KD-234”substance, diluted in distilled water for injections and administered intragastrally through a probe in the dose 50 mg/kg and in 5th studied group – reamberin, administered intraabdominally in the dose 10 ml/kg to animals in 1 hour before ARDS modeling.

Results. It was established, that under conditions of the acute respiratory distress-syndrome SOD, catalase activity and SH-groups content in animals with a different tolerance to hypoxia decrease comparing with the control (р<0,05). In HMT animals group this index is more than in HLT animals. The use of oxygen insufflation under conditions of an experimental distress-syndrome leads to normalization of SOD, catalase activity and SH-groups content in animals with a different tolerance to hypoxia. “KD-234” substance administration is attended by the reliable increase of catalase activity and normalization of the antioxidant-prooxidant index in liver tissues of HMT animals. At reamberin administration SOD activity in liver homogenate grows in both studied groups with the index normalization in HMT animals and the antioxidant-prooxidant index of liver tissues increases (р<0,05).

Conclusions. These data give grounds to consider the use of the combination of “KD -234” substance and reamberin in the complex treatment of ARDS in the experiment as pathogenetically grounded and prospective

Author Biographies

Mariya Marushchak, SHEE «I. Horbachevsky Ternopil State Medical University» Voli sq., 1, Ternopil, Ukraine, 46001

MD, Associate Professor

Department of functional diagnostics and clinical pathophysiology

Samvel Savchuk, SHEE «I. Horbachevsky Ternopil State Medical University» Voli sq., 1, Ternopil, Ukraine, 46001

Department of Anaesthesiology and Reanimatology

Alexander Oliynyk, SHEE «I. Horbachevsky Ternopil State Medical University» Voli sq., 1, Ternopil, Ukraine, 46001

MD, Professor, Head of department 

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Published

2017-06-30

How to Cite

Marushchak, M., Savchuk, S., Hevko, U., & Oliynyk, A. (2017). Peculiarities of antioxidant protection system in the dynamics of acute respiratory distress syndrome development and at different methods of its correction in rats. ScienceRise: Medical Science, (6 (14), 18–23. https://doi.org/10.15587/2519-4798.2017.105583

Issue

No. 6 (14) (2017)

Section

Medical Science

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Copyright (c) 2017 Mariya Marushchak, Samvel Savchuk, Uliana Hevko, Alexander Oliynyk

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