Study on application of pumpkin seed protein isolate in sausage production process
DOI:
https://doi.org/10.15587/2706-5448.2022.255785Keywords:
food industry, meat products, sausage, pumpkin seed protein isolate, orthogonal testAbstract
The object of the research is sausage added with pumpkin seed protein isolate. Recently, plant proteins such as soybean protein and peanut protein are widely applied in meat products. Plant proteins have a lower price and less fat than animal meat, which is benefit for human health. Pumpkin seed protein is one of the new plant proteins, which contained balanced amino acids for human beings, was attracted an increasing interest in food industry. In this study, a new type of sausage was developed by single-factor experiments and orthogonal test. According to the single factor results, the added amount of the pumpkin seed protein isolate (1.5 g/100 g, 2.25 g/100 g, 3.0 g/100 g), lean meat (60 g/100 g, 70 g/100 g, 80 g/100 g), cooking time (35 min, 40 min, 45 min), and baking time (2.5 h) were determined to do the orthogonal test. The orthogonal test showed that the addition amount of pumpkin seed protein isolate had the greatest impact on the sausage quality, followed by the cooking time, and the addition amount of lean meat. The optimal production conditions were pumpkin seed protein isolate of 1.5 g/100 g, lean meat of 80 g/100 g, cooking time of 45 min, and baking time of 2.5 h. Under this condition, the sensory score reached 8.5, and the content of moisture, ash, protein, and fat were 51.16 g/100 g, 2.26 g/100 g, 15.22 g/100 g, and 23.15 g/100 g, respectively. This study can provide a fundamental knowledge for the application of pumpkin seed protein isolate in sausages.
References
- Weiss, J., Gibis, M., Schuh, V., Salminen, H. (2010). Advances in ingredient and processing systems for meat and meat products. Meat Science, 86 (1), 196–213. doi: http://doi.org/10.1016/j.meatsci.2010.05.008
- Xiong, Y. L. (2017). Structure-Function Relationships of Muscle Proteins. Food Proteins and Their Applications. CRC Press, 341–392. doi: http://doi.org/10.1201/9780203755617-12
- Estell, M., Hughes, J., Grafenauer, S. (2021). Plant Protein and Plant-Based Meat Alternatives: Consumer and Nutrition Professional Attitudes and Perceptions. Sustainability, 13 (3), 1478. doi: http://doi.org/10.3390/su13031478
- Asgar, M. A., Fazilah, A., Huda, N., Bhat, R., Karim, A. A. (2010). Nonmeat Protein Alternatives as Meat Extenders and Meat Analogs. Comprehensive Reviews in Food Science and Food Safety, 9 (5), 513–529. doi: http://doi.org/10.1111/j.1541-4337.2010.00124.x
- Broucke, K., Van Poucke, C., Duquenne, B., De Witte, B., Baune, M.-C., Lammers, V. et. al. (2022). Ability of (extruded) pea protein products to partially replace pork meat in emulsified cooked sausages. Innovative Food Science & Emerging Technologies, 78, 102992. doi: http://doi.org/10.1016/j.ifset.2022.102992
- Yuan, X., Zhu, X., Sun, R., Jiang, W., Zhang, D., Liu, H., Sun, B. (2022). Sensory attributes and characterization of aroma profiles of fermented sausages based on fibrous-like meat substitute from soybean protein and Coprinus comatus. Food Chemistry, 373, 131537. doi: http://doi.org/10.1016/j.foodchem.2021.131537
- Ovchinnikova, J. A., Nesterenko, A. A., Oboturova, N. P., Baklanova, O. A., Barybina, L. I., Baklanov, I. S. et. al. (2021). Technological Aspects of the Use of Lentil Proteins in the Production of Raw Smoked Sausages. International Journal Of Pharmaceutical And Phytopharmacological Research, 11 (1), 143–148. doi: http://doi.org/10.51847/kdabnx7j2n
- Chen, L., Chen, W., Ettelaie, R. et. al. (2021). Improving the quality of vegetarian sausage prepared with textured fibril soy protein using oil pre-emulsification. Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering, 37 (13), 291–298.
- Wang, L., Guo, H., Liu, X., Jiang, G., Li, C., Li, X., Li, Y. (2019). Roles of Lentinula edodes as the pork lean meat replacer in production of the sausage. Meat Science, 156, 44–51. doi: http://doi.org/10.1016/j.meatsci.2019.05.016
- Rezig, L., Chibani, F., Chouaibi, M., Dalgalarrondo, M., Hessini, K., Guéguen, J., Hamdi, S. (2013). Pumpkin (Cucurbita maxima) Seed Proteins: Sequential Extraction Processing and Fraction Characterization. Journal of Agricultural and Food Chemistry, 61 (32), 7715–7721. doi: http://doi.org/10.1021/jf402323u
- Kinsella, J. E. (1979). Functional properties of soy proteins. Journal of the American Oil Chemists’ Society, 56 (3Part1), 242–258. doi: http://doi.org/10.1007/bf02671468
- Gao, D., Helikh, A., Duan, Z. (2021). Functional properties of four kinds of oilseed protein isolates. AGRIS, 29 (1), 155–163.
- Wang, L. L., Xiong, Y. L. (2005). Inhibition of Lipid Oxidation in Cooked Beef Patties by Hydrolyzed Potato Protein Is Related to Its Reducing and Radical Scavenging Ability. Journal of Agricultural and Food Chemistry, 53 (23), 9186–9192. doi: http://doi.org/10.1021/jf051213g
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Dan Gao, Anna Helikh, Zhenhua Duan, Yan Liu, Feifei Shang
This work is licensed under a Creative Commons Attribution 4.0 International License.
The consolidation and conditions for the transfer of copyright (identification of authorship) is carried out in the License Agreement. In particular, the authors reserve the right to the authorship of their manuscript and transfer the first publication of this work to the journal under the terms of the Creative Commons CC BY license. At the same time, they have the right to conclude on their own additional agreements concerning the non-exclusive distribution of the work in the form in which it was published by this journal, but provided that the link to the first publication of the article in this journal is preserved.