Evaluation of the activity of energy and biosynthetic processes as a screening for the course of acute experimental pyelonephritis under the influence of pharmacological monitoring

Abstract

In the aspect of studying the prerequisites for the development of diabetic nephropathy, it seems appropriate to study in depth individual enzyme systems that are directly related to the respiratory chain of mitochondria, the implementation of endothelial functions, and biosynthetic processes. Nicotinamide adenine dinucleotide (NAD) has key functions: it is an important coenzyme in the electron transfer reaction and a coenzyme for NAD-dependent enzymes. The role of NAD in electron transfer reactions is significant, since more than 400 enzymes require NAD and nicotine amide adenine dinucleotide phosphate (NADP), mainly to accept or donate electrons for redox reactions, that is, energy reactions in mitochondria and biosynthetic processes. The aim of the study was to study the role of pyridine nucleotides in the pathogenesis of acute pyelonephritis (AP) and concomitant diabetes mellitus in an experiment with the use of drug exposure. It has been established that the use of etiotropic-pathogenetic drug effect (EPDE) in comparison with   etiotropic drug effect (EDE) creates a pronounced normalizing effect on the level and ratio of the forms of the coenzyme system NAD / NADH and NADP / NADPH, contributing to a decrease in the level of the reduced form of NAD increased in AP and type I diabetes and an increase in the content of NADPH in the kidneys, as well as decrease in the level of NADP and NADPH in AP and type II diabetes by 17.9% and 36.3%, The results of experimental biochemical studies obtained by us convincingly prove the expediency of the clinical use of EPDE in order to increase the effectiveness of the corrective effect on pathological changes and prevent the complicated course of diabetic nephropathy.