Enzyme test – early diagnosis of kidney inflammation
DOI:
https://doi.org/10.26641/2307-5279.28.1-4.2024.322090Keywords:
urolithiasis, pyelonephritis, kidney damage markers, NGAL, IL-1β, β2-microglobulin, CRP-1Abstract
The aim of the work is to improve the results of early diagnosis of acute infectious kidney diseases at the molecular level in urolithiasis (urinary stone disease) through the study of enzymatic test indicators. Enzymatic tests (NGAL, IL-1β, β2-microglobulin) were investigated at the molecular level using the IFA method in the urine of patients with urolithiasis. Comparative and prognostic significance of the conducted treatment was established between the groups of patients, and an algorithm was developed based on the results of kidney damage predictors. It was found that the indicators of general laboratory analysis in patients with urolithiasis within the first 24-48 hours do not reliably indicate the absence of an infectious-inflammatory process in the kidneys and the development of renal failure. It was determined that an increase in the inflammation predictor indicators in more than 50% of patients indicates the development of infectious-inflammatory complications within the first 12-24 hours before the occurrence of general laboratory and clinical changes. The assessment of the effectiveness of conservative therapy in groups IA and II revealed that complications of the inflammatory process in the kidneys were observed five times more frequently in group II (comparison) than in group IA. The use of enzymatic tests as markers for early kidney damage allows for the classification of two main groups of patients: those requiring conservative treatment and those requiring urgent surgical intervention. This significantly reduces the frequency of inflammatory, purulent-septic complications and improves the treatment outcomes for patients with upper urinary tract obstruction in urolithiasis.
References
Fink, H. A., Wilt, T. J., Eidman, K. E. & et al. (2013). Medical management to prevent recurrent nephrolithiasis in adults: a systematic review for an Ame¬rican College of Physicians Clinical Guideline. Ann. Intern. Med., 158(7), 535-543. https://doi.org/10.7326/0003-4819-158-7-201304020-00005
Grotsch, H., & Mattenheimer, H. (2003). Methode of Enzymatic analysis. Urology, 3, 42-49.
Gruden, G., Setti, G., Hayward, A., & et. al. (2005). Mechanical stretch induces monocyte chemoattractant activity via an NF - kappa - B-dependent monocyte chemoattractant protein-1 mediated pathway in human mesangial cells: inhibition by rosiglitazone. J. Am. Soc. Nephrol., 16(3), 688-696. https://doi.org/10.168/ASN. 2004030251
Grutznez, F. J. (1982). Diagnostic mit β2-microglobulin. Inn. Med., 9, 45-56.
Honcharenko, I. A. (2003). Study of marker enzymes of damage to structural elements of the nephron and intermediate tissues in closed kidney injury and after extracorporeal shock wave lithotripsy. Odeskyi medychnyi zhurnal, 79(5), 56-59.
Jindal, G., & Ramchandani, P. (2007). Acute flank pain secondary to urolithiasis: radiologic evaluation and alternate diagnoses. Radiol. Clin. North. Am., 45(3), 395-410. https://doi.org/10.1016/j.rcl.2007.04.001
Knoll, T., Musial, A., Trojan, L. & et al. (2003). Measurement of renal anatomy for prediction of lower-pole caliceal stone clearance: Repro-ducibility of different parameters. J. Endourol., 17(7), 447-451. https://doi.org/10.1089/089277903769013577
Kuwabara, T., Mori, K., & Mukoyama, M. (2009). Urinary neutrophil gelatinase – associated lipocalin level reflect damage to glomeruli, proximal tubules, and distal nephrons. Kidney Int., 75(3), 285-294.
https://doi.org/10.1038/ki.2008.499
Lowe, G., & Knudsen, B. E. (2009). Review Ultrasonic, pneumatic and combination intracorporeal lithotripsy for percutaneous nephrolithotomy. J. Endourol., 23(10), 1663-1668. https://doi.org/10.1089/end.2009.1533
Miller, L. G., Mehrotra, R., & Tang, A. W. (2007). Does in vitro fluoroquinolone resistance predict clinical failure in urinary tract infections? Int. J. Antimicrob. Agents., 29(5), 605-607. https://doi.org/10.1016/j.ijantimicag.2006.11.021
Miller, L. S., O'Connell, R. M., Gutierrez, M. A. & et. al. (2006). MyD88 mediates neutrophil recruit¬ment initiated by IL-1 R but not TLR2 activation in immunity against staphylococcus oureus. Immunity., 24(1), 79-91. https://doi.org/10.1016/j.immuni.2005.11.011
Mishra, J., & et. al. (2003). Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. Clin. J. Am. Soc. Nephrol., 14(10), 2534-2343. https://doi.org/10.1097/01.ASN.0000088027.54400.C6
Mishra, J., Mori, K., Devarajan, P., & Kelly, C. (2006). Kidney NGAL is a novel early marker of acute injury following transplantation. Pediatr. Nephrol., 21(6), 856-863. https://doi.org/10.1007/s00467-006-0055-0
Moe, O. W. (2006). Kidney stones: pathophysiology and medical management. Lancet, 367(9507), 333-344.
https://doi.org/10.1016/S0140-6736(06)68071-9
Myhal, L. Ya., Korol, L. V., Tumanova, L. Ye. & et al. (2005). Urine transaminase as a marker enzyme for kidney pathology in pregnant women with pyelonephritis. Lab. diagnostics, 4(34), 24-27.
Pasiechnikov, S. P., & et al. (2013). Urologiya: textbook. Vinnytsia: Nova knyha, 154-224.
Pasiechnikov, S. P., Saidakova, N. O., & Glebov, A.S. (2010). The current state of the problem of kidney and urinary tract infection in Ukraine. Urologiya, (14), 72-74.
Ray, A. A., Ghiculete, D., Pace, K. T., & Honey, R. J. (2010). Limitations to ultrasound in the detection and measurement of urinary tract calculi. Urology, 76(2), 295-300. https://doi.org/10.1016/j.urology.2009.12.015
Ruggera, L., Beltrami, P., Ballario, R., & et al. (2005). Impact of anatomical pyelocaliceal topography in the treatment of renal lower calyces stones with extracorporeal shock wave lithotripsy. Int. J. Urol., 12(6), 525-532. https://doi.org/10.1111/j.1442-2042.2005.01101.x
Shen, S. J., Hu, Z. X., & Li, O. H. (2014). Implications of the changes in serum neutrophil gelati¬nase – associated lipocalin and Cystatin C in patients with chronic kidney disease. Nephrology, 19(3), 29-35. https://doi.org/10.1111/nep.12203
Vozianov, A. F., Sernyak, P. S., & Baylo, V. D. (1984). Surgical treatment of recurrent nephrolithiasis. Kyiv: Zdorov’e, 4-57.
Vozianov, O. F., & Liulko, O. V. (2002). Urology: textbook. 2nd ed., revised and enlarged. Dnipro: Dnipro-VAL, 385-603.
Vozianov, O. F., Pasiechnikov, S. P., Saidako-va, N. O., & Dmytryshyn, S. P. (2010). Dynamics of morbidity and prevalence of urolithiasis among the adult population of Ukraine. Zdorov'e muzhchyny, (2), 17-24.
Yermolenko, T. I., & Zhulay, T. S. (2011). Pharmaco¬economic evaluation of conservative treatment of UUTC using a new domestic urolytic agent "Flarosuktsyn". Klinichna farmatsiia, 15(3), 17-20.
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