Amphibian skin glands secretions affect plasma coagulation tests

Authors

  • Iryna Udovychenko Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601, Ukraine https://orcid.org/0000-0002-4586-1424
  • Yuliia Dudkina Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601, Ukraine https://orcid.org/0000-0002-4636-6230
  • Denys Oliinyk Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601, Ukraine https://orcid.org/0000-0002-2814-460X
  • Oleksandra Oskyrko Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601, Ukraine https://orcid.org/0000-0003-0092-4193
  • Oleksii Marushchak I. I. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine B. Khmelnytskogo str., 15, Kyiv, Ukraine, 01030, Ukraine https://orcid.org/0000-0001-9380-5593
  • Tetiana Halenova Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601, Ukraine https://orcid.org/0000-0003-2973-2646

DOI:

https://doi.org/10.15587/2519-8025.2018.148075

Keywords:

amphibians, skin gland secretions, skin poisons, plasma coagulation tests, coagulation hemostasis

Abstract

According to the extremely high mortality and disability rates associated with the abnormal functioning of the hemostasis system, the search for new approaches for the prevention and treatment of these conditions is one of the most acute problems of modern biochemistry. The active components of reptile poisons are actively used in the treatment of these diseases, but the study of the effects of amphibians’ skin glands secretion on the functioning of hemostasis system has not yet been carried out. So, the aim of this work was to assess the effects of the components of amphibian skin secretions on the functioning of the coagulation link of the hemostasis system.

Methods. In this study the skin secretions of ten Ukrainian species of amphibians were collected: B. bombina, B. variegata, B. bufo, B. viridis, R. temporaria, P. ridibundus, P. esculentus, P. fuscus, S. salamandra and the hybrid of B. bombina and B. variegatа. The samples of crude skin secretions were prepared. The activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombin time (PT) tests were conducted in vitro using the coagulation analyzer (Rayto RT-2201C, China) and the standard set of reagents (RENAM, Russian Federation).

Results. It was established that the components of crude skin secretions of B. bombina, B. variegata, their hybrid, R. temporaria and P. ridibundus prolonged the aPTT of clotting plug formation. The components of skin secretions of B. viridis, P. esculentus, P. fuscus and S. salamandra prolonged the TT of clotting plug formation.

Conclusions. The fact that some amphibian species prolonged the aPTT and TT of clotting plug formation could be the indicator of the presence of inhibitors of certain factors of coagulation hemostasis or be the reason of the degradation of the components of coagulation hemostasis by active components of skin secretion. Such results prove that the amphibian crude skin secretions are a potential source of the compounds that can affect the hemostasis system. The identification of an active component and the elaboration of its mechanism of action are required in further investigations

Author Biographies

Iryna Udovychenko, Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601

Postgraduate student

Department of Biochemistry

Oleksii Marushchak, I. I. Schmalhausen Institute of Zoology of National Academy of Sciences of Ukraine B. Khmelnytskogo str., 15, Kyiv, Ukraine, 01030

Postgraduate student

Department of Animal monitoring and Conservation

Tetiana Halenova, Educational and Scientific Centre "Institute of Biology and Medicine" of Taras Shevchenko National University of Kyiv Volodymyrska str., 64/13, Kyiv, Ukraine, 01601

PhD, Seniour resercher

Educational and experimental laboratory «Physical and chemical biology»

References

  1. Piepoli, M. F., Corrà, U., Abreu, A., Cupples, M., Davos, C., Doherty, P. et. al. (2015). Challenges in secondary prevention of cardiovascular diseases. International Journal of Cardiology, 180, 114–119. doi: http://doi.org/10.1016/j.ijcard.2014.11.107
  2. Yusuf, S. (2018). Improving worldwide access to inexpensive and effective treatments for common cardiovascular diseases. European Heart Journal Supplements, 20, 18–22. doi: http://doi.org/10.1093/eurheartj/suy005
  3. Clarke, B. T. (2007). The natural history of amphibian skin secretions, their normal functioning and potential medical applications. Biological Reviews, 72 (3), 365–379. doi: http://doi.org/10.1111/j.1469-185x.1997.tb00018.x
  4. Xu, X., Lai, R. (2015). The Chemistry and Biological Activities of Peptides from Amphibian Skin Secretions. Chemical Reviews, 115 (4), 1760–1846. doi: http://doi.org/10.1021/cr4006704
  5. Montecucchi, P. C., Gozzini, L., Erspamer, V., Melchiorri, P. (2009). Primary structure of tryptophan-containing peptides from skin extracts of Phyllomedusa rhodei (tryptophyllins). International Journal of Peptide and Protein Research, 23 (3), 276–281. doi: http://doi.org/10.1111/j.1399-3011.1984.tb02720.x
  6. Barberio, C., Delfino, G., Mastromei, G. (1987). A low molecular weight protein with antimicrobial activity in the cutaneous “venom” of the yellow-bellied toad (Bombina variegata pachypus). Toxicon, 25 (8), 899–909. doi: http://doi.org/10.1016/0041-0101(87)90250-9
  7. Gomes, A., Giri, B., Saha, A., Mishra, R., Dasgupta, S., Debnath, A. (2007). Bioactive molecules from amphibian skin: Their biological activities with reference to therapeutic potentials for possible drug development. Indian Journal of Experimental Biology, 45 (71), 579–590.
  8. Erspamer, V. (1999). Biogenic amines and active polypeptides of amphibian skin. Biological reviews of the Cambridge Physiological Society, 172 (3), 211–220.
  9. Vigerelli, H., Sciani, J. M., Eula, M. A. C., Sato, L. A., Antoniazzi, M. M., Jared, C., Pimenta, D. C. (2018). Biological Effects and Biodistribution of Bufotenine on Mice. BioMed Research International, 2018, 1–10. doi: http://doi.org/10.1155/2018/1032638
  10. Cei, J. M., Erspamer, V., Roseghini, M. (1967). Taxonomic and Evolutionary Significance of Biogenic Amines and Polypeptides Occurring in Amphibian Skin. I. Neotropical Leptodactylid Frogs. Systematic Zoology, 16 (4), 328–342. doi: http://doi.org/10.2307/2412152
  11. Cunha Filho, G. A., Schwartz, C. A., Resck, I. S., Murta, M. M., Lemos, S. S., Castro, M. S. et. al. (2005). Antimicrobial activity of the bufadienolides marinobufagin and telocinobufagin isolated as major components from skin secretion of the toad Bufo rubescens. Toxicon, 45 (6), 777–782. doi: http://doi.org/10.1016/j.toxicon.2005.01.017
  12. Daly, J. W., Highet, R. J., Myers, C. W. (1984). Occurrence of skin alkaloids in non-dendrobatid frogs from Brazil (Bufonidae), Australia (Myobatrachidae) and Madagascar (Mantellinae). Toxicon, 22 (6), 905–919. doi: http://doi.org/10.1016/0041-0101(84)90182-x
  13. Azevedo, A. P. S., Farias, J. C., Costa, G. C., Ferreira, S. C. P., Aragão-Filho, W. C., Sousa, P. R. A. et. al. (2007). Anti-thrombotic effect of chronic oral treatment with Orbignya phalerata Mart. Journal of Ethnopharmacology, 111 (1), 155–159. doi: http://doi.org/10.1016/j.jep.2006.11.005
  14. Koch, E., Biber, A. (2007). Treatment of rats with the Pelargonium sidoides extract EPs® 7630 has no effect on blood coagulation parameters or on the pharmacokinetics of warfarin. Phytomedicine, 14, 40–45. doi: http://doi.org/10.1016/j.phymed.2006.11.026
  15. Nikolaieva, I., Dudkina, Yu., Oliinyk, D., Oskyrko, O., Marushchak, O., Halenova, T., Savchuk, O. (2018). Amphibian skin secretions: a potential source of proteolytic enzymes. Biotechnologia Acta. In press.

Downloads

Published

2018-11-27

How to Cite

Udovychenko, I., Dudkina, Y., Oliinyk, D., Oskyrko, O., Marushchak, O., & Halenova, T. (2018). Amphibian skin glands secretions affect plasma coagulation tests. ScienceRise: Biological Science, (5 (14), 36–40. https://doi.org/10.15587/2519-8025.2018.148075

Issue

No. 5 (14) (2018)

Section

Biological Sciences

License

Copyright (c) 2018 Iryna Udovychenko, Yuliia Dudkina, Denys Oliinyk, Oleksandra Oskyrko, Oleksii Marushchak, Tetiana Halenova

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.

Authors, who are published in this journal, agree to the following conditions:

1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.

 2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.