Effect of oligoribonucleotides with D-mannitol complexes on oxidative stress indicators against thioacetamide-induced liver fibrosis

Authors

  • Tetiana Marchyshak Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143, Ukraine https://orcid.org/0000-0001-7141-1198
  • Tetiana Yakovenko Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143, Ukraine https://orcid.org/0000-0002-7296-5755
  • Zenoviy Tkachuk Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143, Ukraine https://orcid.org/0000-0002-0433-007X

DOI:

https://doi.org/10.15587/2519-8025.2020.214418

Keywords:

oxidative stress, antioxidant defence system, complexes of oligoribonucleotides with D-mannitol, liver fibrosis

Abstract

The aim of the study. To determine the effect of oligoribonucleotides-D-mannitol complexes (ORN-D-M) on the indicators of oxidative destruction of biomolecules and the antioxidant system of cells in thioacetamide (TAA)-induced liver fibrosis.

Materials and methods. Liver fibrosis was induced for 8 weeks by intraperitoneal administration of TAA (150 mg/kg body weight). ORN-D-M (200 mg/kg per os) was administered orally during intoxication. At the end of the experiment, the liver was excised and examined for the content of oxidative stress products and the activity of antioxidant enzymes. Data were analyzed using the ANOVA test followed by Tukey post hoc testing.

Results. It is shown that the monotherapeutic treatment of ORN-D-M in TAA-induced liver fibrosis has a pronounced protective effect, which is manifested in the reduction of oxidative stress. ORN-D-M led to the attenuation of free radical damage of biopolymers, which was manifested in a decrease in the levels of peroxidation products of lipids and proteins with a simultaneous increase in the level of protein thiol groups and reduced glutathione. In addition, treatment with complexes increased the activity of the antioxidant defence system of cells.

Conclusions. The obtained results indicate that ORN-D-M complexes have a potential hepatoprotective effect in TAA-induced liver fibrosis. The complexes are able to effectively reduce the indicators of oxidative damage of biomolecules with a simultaneous increase in the activity of enzymes of the antioxidant system in TAA-induced fibrosis

Author Biographies

Tetiana Marchyshak, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143

Junior Researcher

Department of Enzymology of Protein Synthesis

Tetiana Yakovenko, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143

Leading Engineer

Department of Enzymology of Protein Synthesis

Zenoviy Tkachuk, Institute of Molecular Biology and Genetics of National Academy of Sciences of Ukraine Akademika Zabolotnoho str., 150, Kyiv, Ukraine, 03143

PhD, Senior Researcher

Department of Enzymology of Protein Synthesis

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Published

2020-06-30

How to Cite

Marchyshak, T., Yakovenko, T., & Tkachuk, Z. (2020). Effect of oligoribonucleotides with D-mannitol complexes on oxidative stress indicators against thioacetamide-induced liver fibrosis. ScienceRise: Biological Science, (3 (24), 35–40. https://doi.org/10.15587/2519-8025.2020.214418

Issue

No. 3 (24) (2020)

Section

Biological Sciences

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Copyright (c) 2020 Tetiana Marchyshak, Tetiana Yakovenko, Zenoviy Tkachuk

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