Research of influence of contrasting trophic conditions of vernalization on the mitotic activity of meristems, growth and development of winter wheat

Authors

DOI:

https://doi.org/10.15587/2519-8025.2017.99664

Keywords:

Triticum aestivum L., vernalization, trophic factors, proliferative activity, growth reaction, development rates

Abstract

The work is devoted to the most important physiological and selective sign of winter soft wheat Triticum aestivum L. – vernalization.

Aim. The main aim of the work was the study of trophic factor influence on the effectiveness of vernalization process.

Methods. In experiments were used the varieties of winter wheat – Statna and Doridna. The contrasting conditions of trophic support were created by vernalization on integral seeds (control) and isolated buds (experiment) adding water and 3-% solution of saccharose during 45 days at temperature 3±1°С. In experiments there was studied the influence of contrasting trophic conditions of vernalization on the mitotic activity of root meristems of vernalized sprouts, their growth reaction and rates of ontogenetic development of plants, cultivated from vernalized sprouts. All experiments were carried out under conditions of vegetative experiment in factorostatic chamber of the department of physiology and biochemistry of plants and microorganisms of Kharkiv national university, named after V.N. Karazin.

Results. According to the results of researches, it was established that the growth reaction of sprouts at vernalization depends on their variety specific characteristics and on trophic factors presence. It was demonstrated that 3% solution of saccharose at vernalization stimulates proliferative activity of root meristems of both winter wheat varieties but doesn’t influences the parameter – duration of mitosis phases. The presence of trophic factors in sprouts at vernalization conditions the speed of phenophases passage and transfer to generative development of both winter wheat varieties. The vernalized sprouts of Doridna variety are characterized with higher parameters of mitotic activity of root apical meristems, accumulation of biomass of sprouts at vernalization influence and longer vegetative period comparing with Statna.

Conclusion. The trophic conditions of vernalization process cause proliferative activity of meristems, growth reaction of sprouts and speed of transfer to generative development that is effectiveness of vernalization

Author Biographies

Olga Avksentiievа, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraine, 61022

PhD, Associate professor

Department of Plant and Microorganisms Physiology and Biochemistry 

Victoria Shulik, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraine, 61022

Department of Plant and Microorganisms Physiology and Biochemistry

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Published

2017-04-26

How to Cite

Avksentiievа O., & Shulik, V. (2017). Research of influence of contrasting trophic conditions of vernalization on the mitotic activity of meristems, growth and development of winter wheat. ScienceRise: Biological Science, (2 (5), 4–9. https://doi.org/10.15587/2519-8025.2017.99664

Issue

Section

Biological Sciences