The lysozyme activity in liver and сolon dysbiozis after experimental antibiotic therapy
DOI:
https://doi.org/10.15587/2519-8025.2017.113060Keywords:
antibiotics, lysozyme, urease, dysbiosis, blood serum, liver, large intestine, ratsAbstract
As far as antibiotics cause dysbiotic and hepatotoxic side effects, there appeared the interest to the experimental study of the influence of antibiotics on the antimicrobial function of the liver and colon microbiota, especially lysozyme activity in tissues of rats.
Aim –is to study the influence of several antibiotics on lysozyme activity in the liver, blood serum and colon.
Methods. The studies were realized on rats of Wistar line, that were administered with soluble forms of antibiotics with drinking water: cefixime (20 mg/kg), or sumamed (25 mg/kg), or amoxiclav (40 mg/kg), or lincomycin (60 mg/kg) during 5 days, or antihelicobacter complex (omeprasole 1,33 mg/kg, amoxil-К 50 mg/kg and clarithromycin 7,5 mg/kg) for 8 days. Doses corresponded to developers’ recommendations. Euthanasia of animals was realized under thiopental anesthesia (40 mg/kg) in 5 days after the last intake of antibiotics. Lysozyme and urease activity was determined the blood serum, homogenates of the liver and colon mucosa, and the dysbiosis degree was calculated by their level according to Levitsky by the enzymatic method. The results were processed using Student t-criterion.
Results. Antibiotics cause the decrease of lysozyme activity in the colon mucosa, blood serum and liver of rats. This factor decreases most after using lincomycin and antihelicobacter complex, insignificantly – after amoxiclav. Synchronously, urease activity in the colon mucosa, blood serum and liver and also the dysbiosis degree in the colon increase. Lincomycin and antihelicobacter complex essentially raised these indices. The course of amoxiclav, in opposite, caused the minimal effect on urease activity and dysbiosis degree. The obtained results allow to presuppose that reproduction of pathogenic and conventionally pathogenic microflora in the mucosa of the large intestine, established by the increase of urease activity, may be a result of weakening of activity of the antimicrobial factor of lysozyme of the liver and colon after the realized antibiotic therapy. It must be taken into account at elaborating the prophylaxis of side effects of antibiotics
References
- Babak, O. Ya. (2008). Lekarstvennyie porazheniya pecheni: voprosyi teorii i praktiki [Liver medications: theory and practice]. Lіky Ukrainy, 118, 96–101.
- Bоndаrеnkо, V. М., Vorobev, А. А. (2004). Disbiozyi i preparatyi s probioticheskoy funktsiey [Dysbiosis and preparations with probiotic function]. Zhurnal mikrobiologii, epidemiologii, immunologii, 1, 84–92.
- Ryabichenko, Е. V., Bоndаrеnkо, V. М. (2007). Rol kishechnoy bakterialnoy autofloryi i eyo endotoksina v patologii cheloveka [The role of intestinal bacterial autoflora and its endotoxin in human pathology]. Zhurnal mikrobiologii, epidemiologii, immunologii, 3, 103–111.
- Wang, X., Quinn, P. (2010). Endotoxins: structure, function and recognition. Seria: Subcellular Biochemistry, 53, 415. doi: 10.1007/978-90-481-9078-2
- Skrypkina, I. N., Maslova, A. S. (2009). Rol narusheniy mikrobiotsenoza kishechnika v patogeneze zabolevaniy vnutrennih organov [The role of disorders of intestinal microbiocenosis in the pathogenesis of diseases of internal organs]. Lіky Ukrainy, 6 (132), 65–71.
- Levitskiy, A. P., Demyanenko, S. A., Tsiselskiy, Yu. V. (2011). Antimikrobnaya funktsiya pecheni [Antimicrobial liver function]. Оdеssа: KP “Odesskaya gorodskaya tipografiya”, 141.
- Yeger, L. (Ed.) (1990). Klinicheskaya immunologiya i allergologiya. Vol. 1 [Clinical immunology and allergology. Vol. 1]. Moscow: Meditsina, 528.
- Vershigora, A. E. (1980). Osnovyi immunologii [Fundamentals of Immunology]. Kyiv: Vyisshaya shkola, 490.
- Savilov, P. N., Dyachkova, S. Ya., Kuzmina, N. I. (2005). Lizotsimobogaschayuschaya funktsiya pecheni posle chastichnoy gepatektomii i giperbaricheskoy oksigenatsii [Lysozyme-enriching function of the liver after partial hepatectomy and hyperbaric oxygenation]. Voprosyi biologicheskoy, meditsinskoy i farmatsevticheskoy himii, 4, 35–39.
- Turovskiy, A. V., Dyachkova S. Ya. (2013). Vliyanie razlichnyih ob'Yomov rezektsii pecheni na fone CCl4-gepatita na aktivnost gumoralnyih faktorov estestvennoy rezistentnosti organizma [The effect of various volumes of liver resection on the background of CCl4-hepatitis on the activity of humoral factors of natural resistance of the organism]. Vestnik VGU. Seriya: Himiya. Biologiya. Farmatsiya, 1, 230–234.
- European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe (1986). Strasbourg, 52.
- Levitskiy, A. P. (2005). Lizotsim vmesto antibiotikov [Lysozyme instead of antibiotics]. Оdеssа: KP “Odesskaya gorodskaya tipografiya”, 74.
- Gavrikova, L. M., Segen, I. T. (1996). Ureaznaya aktivnost rotovoy zhidkosti u bolnyih s ostroy odontogennoy infektsiey chelyustno-litsevoy oblasti [Urease activity of the oral fluid of patients with acute odontogenic infection and maxillofacial]. Stomatologiya, Spetsial'nyi vypusk, 49–50.
- Levitskiy, A. P., Makarenko, O. A., Selivanskaya, I. A. et. al. (2007) Fermentativnyy metod opredeleniya disbioza polosti rta dlya skrininga pro- i prebiotikov [Enzymatic methods for determination of oral dysbiosis for screening pro- and prebiotics]. Kyiv: GFC, 22.
- Sernov, L. N., Gatsura, V. V. (2000). Elementyi eksperimentalnoy farmakologii [Elements of Experimental Pharmacology]. Moscow: Meditsina, 352.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Anatoliy Levitsky, Olga Makarenko, Наnna Maikova, Tetiana Kolomiichuk, Olga Pavlichenko
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
