Cytogenetic effects in cancer patients lymphocytes depending on the radiation source and the locality of radiation exposure in experiment ex vivo

Authors

  • Nataliya Maznyk State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024, Ukraine https://orcid.org/0000-0002-3216-1330
  • Tetiana Sypko State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024, Ukraine https://orcid.org/0000-0002-1788-9235
  • Viktor Starenkiy State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024, Ukraine https://orcid.org/0000-0002-6600-3381

DOI:

https://doi.org/10.15587/2519-8025.2019.178907

Keywords:

chromosome aberrations, cancer patients, lymphocytes, ex vivo experiment, partial-body simulation, gamma-irradiation, megavolt irradiation on linear accelerator

Abstract

Aims: Estimation of the cytogenetic lesions yield and their distribution among cells in donor lymphocytes of cancer patients with different tumor localizations depending on the source of radiation and the locality of radiation exposure in a therapeutically significant dose in ex vivo experiment.

Methods: Cytogenetic analysis was performed in lymphocytes of 30 oncogynecological patients, lung cancer patients and head and neck cancer patients before the start of radiation treatment. Whole peripheral blood was irradiated at 2 Gy dose with a further simulation of partial body irradiation using gamma-irradiation 60Co on the ROKUS-AM and megavolt irradiation on the linear accelerator Clinac 600C.

Results of research: An increase of radiation-specific chromosome damage frequency after gamma- and megavolt irradiation of cancer patients’ lymphocytes at 2 Gy dose was shown. With the absence of dependence on the tumor localization the statistically significant excess of the chromosome exchanges level due to irradiation on linear accelerator in compare with gamma-irradiation was found. At 2 Gy dose point with a simulation of partial body irradiation a similar dependence on the applied source was observed. So, the increase of the chromosome type aberrations level was due to 2,5-fold increase of the dicentric and ring chromosomes number under the gamma-irradiation and 5-fold under megavolt irradiation. For local irradiation simulation for both sources the chromosome aberrations level significantly exceeded the values of the zero point, and the dicentrics distribution among cells was over-dispersed according to Poisson statistic.

Conclusion: Cytogenetic studies in ex vivo experiment showed that in donors’ lymphocytes, regardless of the tumor location, megavolt irradiation demonstrated a more genotoxic effect in compare with gamma-irradiation. The data obtained indicated that the proposed test experiment of ex vivo irradiation with partial body radiation simulation can be successfully used to detect the fact of irradiation and to confirm, if present, its locality. The study results will contribute to the improvement of the radiobiological basis of cancer patients’ radiation treatment and can be of use for the development of approaches to the individualization of therapeutic irradiation

Author Biographies

Nataliya Maznyk, State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024

Doctor of Biological Sciences, Head of Laboratory

Laboratory of Radiation Cytogenetics

Tetiana Sypko, State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024

Researcher

Laboratory of Radiation Cytogenetics

Viktor Starenkiy, State Institution «Grigoriev Institute for Medical Radiology of National Academy of Medical Science of Ukraine» Pushkinska str., 82, Kharkiv, Ukraine, 61024

MD, Professor, Head of Department

Department of Radiation Therapy

References

  1. Ovchinnikov, V. A., Uglyanitsa, K. N., Volkov, V. N. (2010). Modern methods of radiotherapy of oncological patients. Journal of the Grodno State Medical University, 1 (29), 93–97.
  2. Trofimova, O. P., Tkachev, S. I., Yuryeva, T. V. (2013). Past and present of radiotherapy in management of malignancies. Clinical Oncohematology, 6 (4), 355–364.
  3. The timely delivery of radical radiotherapy: guidelines for the management of unscheduled treatment interruptions (2019). London: The Royal College of Radiologists, 37.
  4. Diegues, S. S., Ciconelli, R. M., Segreto, R. A. (2008). Causes of unplanned interruption of radiotherapy. Radiologia Brasileira, 41 (2), 103–108. doi: http://doi.org/10.1590/s0100-39842008000200009
  5. Demidov, V. V., Melnov, S. B., Ryibalchenko, O. A. (2002). Otsenka individualnoy radiochuvstvitelnosti u raznovozrastnyih grupp naseleniya respubliki Belarus. Saharovskie chteniya. Minsk, 83–85.
  6. Sharygin, V. L. (2018). The Use of EPR Spectroscopy at System Analysis of Organism Radiosensitivity / Radioresistance. Experience and Tendencies. Radiation biology. Radioecology, 58 (5), 463–476. doi: http://doi.org/10.1134/s0869803118050168
  7. Andreassen, C. N. (2005). Can risk of radiotherapy-induced normal tissue complications be predicted from genetic profiles? Acta Oncologica, 44 (8), 801–815. doi: http://doi.org/10.1080/02841860500374513
  8. Andreassen, C. N., Alsner, J. (2009). Genetic variants and normal tissue toxicity after radiotherapy: A systematic review. Radiotherapy and Oncology, 92 (3), 299–309. doi: http://doi.org/10.1016/j.radonc.2009.06.015
  9. Rodrigues, A. S., Oliveira, N. G., Gil, O. M., Léonard, A., Rueff, J. (2005). Use of cytogenetic indicators in radiobiology. Radiation Protection Dosimetry, 115 (1-4), 455–460. doi: http://doi.org/10.1093/rpd/nci072
  10. Greulich-Bode, K. M., Zimmermann, F., Muller, W.-U., Pakisch, B., Molls, M., Wurschmidt, F. (2012). Clinical, Molecular- and Cytogenetic Analysis of a Case of Severe Radio- Sensitivity. Current Genomics, 13 (6), 426–432. doi: http://doi.org/10.2174/138920212802510475
  11. Grekhova, A. K., Pustovalova, M. V., Eremin, P. S., Yashkina, E. I., Osipov, A. N. (2018). The Problem of Analysis of Post-Radiation Changes in the Number of γH2AX Foci in Asynchronous Cell Population. Radiation biology. Radioecology, 58 (5), 484–489. doi: http://doi.org/10.1134/s0869803118050077
  12. Filushkin, I. V., Nugis, V. Yu., Chistopolskij, A. S. (1999). Comparative cytogenetic analysis of cultures of irradiated lymphocytes and mixed cultures of irradiated and non irradiated cells. Medical Radiology and Radiation Safety, 44 (3), 19–26.
  13. Semenov, A. V., Vorobtsova, I. E. (2016). Chastota hromosomnyih aberratsiy v limfotsitah perifericheskoy krovi bolnyih s solidnyimi opuholyami. Voprosyi onkologii, 62 (3), 485–489.
  14. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies (2011). Vienna: International Atomic Energy Agency, 229.
  15. Higueras, M., González, J. E., Di Giorgio, M., Barquinero, J. F. (2018). A note on Poisson goodness-of-fit tests for ionizing radiation induced chromosomal aberration samples. International Journal of Radiation Biology, 94 (7), 656–663. doi: http://doi.org/10.1080/09553002.2018.1478012
  16. Atramentova, L. A, Utevskaya, O. M. (2008). Statisticheskie metodyi v biologii. Gorlovka: Vydavnytstvo Likhtar, 248.
  17. Senthamizhchelvan, S., Pant, G. S., Rath, G. K., Julka, P. K., Nair, O. (2009). Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation. Physica Medica, 25 (2), 82–87. doi: http://doi.org/10.1016/j.ejmp.2008.05.004
  18. Miszczyk, J., Rawojć, K., Panek, A., Swakoń, J., Prasanna, P. G., Rydygier, M. (2015). Response of human lymphocytes to proton radiation of 60MeV compared to 250kV X-rays by the cytokinesis-block micronucleus assay. Radiotherapy and Oncology, 115 (1), 128–134. doi: http://doi.org/10.1016/j.radonc.2015.03.003
  19. Puig, R., Pujol, M., Barrios, L., Caballín, M. R., Barquinero, J.-F. (2016). Analysis of α-particle-induced chromosomal aberrations by chemically-induced PCC. Elaboration of dose-effect curves. International Journal of Radiation Biology, 92 (9), 493–501. doi: http://doi.org/10.1080/09553002.2016.1206238

Downloads

Published

2019-10-11

How to Cite

Maznyk, N., Sypko, T., & Starenkiy, V. (2019). Cytogenetic effects in cancer patients lymphocytes depending on the radiation source and the locality of radiation exposure in experiment ex vivo. ScienceRise: Biological Science, (3 (18), 25–31. https://doi.org/10.15587/2519-8025.2019.178907

Issue

No. 3 (18) (2019)

Section

Biological Sciences

License

Copyright (c) 2019 Nataliya Maznyk, Tetiana Sypko, Viktor Starenkiy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.

Authors, who are published in this journal, agree to the following conditions:

1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.

 2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.