Research of influence of contrasting trophic conditions of vernalization on the mitotic activity of meristems, growth and development of winter wheat

Authors

DOI:

https://doi.org/10.15587/2519-8025.2017.99664

Keywords:

Triticum aestivum L., vernalization, trophic factors, proliferative activity, growth reaction, development rates

Abstract

The work is devoted to the most important physiological and selective sign of winter soft wheat Triticum aestivum L. – vernalization.

Aim. The main aim of the work was the study of trophic factor influence on the effectiveness of vernalization process.

Methods. In experiments were used the varieties of winter wheat – Statna and Doridna. The contrasting conditions of trophic support were created by vernalization on integral seeds (control) and isolated buds (experiment) adding water and 3-% solution of saccharose during 45 days at temperature 3±1°С. In experiments there was studied the influence of contrasting trophic conditions of vernalization on the mitotic activity of root meristems of vernalized sprouts, their growth reaction and rates of ontogenetic development of plants, cultivated from vernalized sprouts. All experiments were carried out under conditions of vegetative experiment in factorostatic chamber of the department of physiology and biochemistry of plants and microorganisms of Kharkiv national university, named after V.N. Karazin.

Results. According to the results of researches, it was established that the growth reaction of sprouts at vernalization depends on their variety specific characteristics and on trophic factors presence. It was demonstrated that 3% solution of saccharose at vernalization stimulates proliferative activity of root meristems of both winter wheat varieties but doesn’t influences the parameter – duration of mitosis phases. The presence of trophic factors in sprouts at vernalization conditions the speed of phenophases passage and transfer to generative development of both winter wheat varieties. The vernalized sprouts of Doridna variety are characterized with higher parameters of mitotic activity of root apical meristems, accumulation of biomass of sprouts at vernalization influence and longer vegetative period comparing with Statna.

Conclusion. The trophic conditions of vernalization process cause proliferative activity of meristems, growth reaction of sprouts and speed of transfer to generative development that is effectiveness of vernalization

Author Biographies

Olga Avksentiievа, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraine, 61022

PhD, Associate professor

Department of Plant and Microorganisms Physiology and Biochemistry 

Victoria Shulik, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraine, 61022

Department of Plant and Microorganisms Physiology and Biochemistry

References

  1. Morgun, V. V., Shvartau, V. V., Kiriziy, D. A. (2010). Fiziologicheskie osnovyi formirovaniya vyisokoy produktivnosti zernovyih zlakov [The physiological basis for the formation of high productivity of cereals]. Physiology and biochemistry of cult. plants, 42 (5), 371–392.
  2. Dennis, E. S., Peacock, W. J. (2009). Vernalization in cereals. Journal of Biology, 8 (6), 57. doi: 10.1186/jbiol156
  3. Stelmah, A. F., Fayt, V. I., Martyinyuk, V. R. (2000). Geneticheskie sistemyi tipa i kontrolya skorosti razvitiya myagkoy pshenitsyi [Genetic type and control the rate of development of soft wheat]. Cytology and Genetics, 34 (2), 39–45.
  4. Avksenteva, O. A., Shulik, V. V., Zhmurko, V. V. (2015). Allelnyie variantyi genov VRN i tempyi razvitiya izogennyih liniy pshenitsyi [Allelic variants of genes VRN and the pace of development of isogenic lines of wheat]. Factors of experimental evolution of organisms, 17, 17–21.
  5. Song, J., Angel, A., Howard, M., Dean, C. (2012). Vernalization – a cold-induced epigenetic switch. Journal of Cell Science, 125 (16), 3723–3731. doi: 10.1242/jcs.084764
  6. Scherban, A. S., Salina, E. A. (2013). Epigeneticheskaya regulyatsiya ekspressii genov yarovizatsii [Epigenetic regulation of gene expression of vernalization]. Cytology, 55 (4), 234–237.
  7. Avksenteva, O. A., Zhmurko, V. V. (2011). Fiziologiya tsveteniya [Physiology of flowering]. Kharkiv: V. N. Karazin KhNU, 130.
  8. Aoki, N., Scofield, G. N., Wang, X.-D., Offler, C. E., Patrick, J. W., Furbank, R. T. (2006). Pathway of Sugar Transport in Germinating Wheat Seeds. Plant Physiology, 141 (4), 1255–1263. doi: 10.1104/pp.106.082719
  9. Rolland, F., Moore, B., Sheen, J. (2002). Sugar sensing and signaling in plants. The Plant Cell, 14, 185–205.
  10. Eveland, A. L., Jackson, D. P. (2011). Sugars, signalling, and plant development. Journal of Experimental Botany, 63 (9), 3367–3377. doi: 10.1093/jxb/err379
  11. Riou-Khamlichi, C., Menges, M., Healy, J., Murray, J. (2000). Sugar control of the plant cell cycle: differential regulation of Arabidopsis D-type cyclin gene expression. Molecular and Cellular Biology, 20 (13), 4513–4521.
  12. O’Hara, L. E., Paul, M. J., Wingler, A. (2013). How Do Sugars Regulate Plant Growth and Development? New Insight into the Role of Trehalose-6-Phosphate. Molecular Plant, 6 (2), 261–274. doi: 10.1093/mp/sss120
  13. Koch, K. (2004). Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. Current Opinion in Plant Biology, 7 (3), 235–246.
  14. Henderson, I. R., Shindo, C., Dean, C. (2003). The Need for Winter in the Switch to Flowering. Annual Review of Genetics, 37 (1), 371–392. doi: 10.1146/annurev.genet.37.110801.142640
  15. Sung, S., Amasino, R. M. (2005). Remembering winter: Toward a Molecular Understanding of Vernalization. Annual Review of Plant Biology, 56 (1), 491–508. doi: 10.1146/annurev.arplant.56.032604.144307
  16. Ivanov, V. B. (2011). Kletochnyie mehanizmyi rosta rasteniy [Cellular mechanisms of plant growth ]. Moscow: Science, 104.
  17. Kumar, S., Arya, S., Roy, B., Singh, A. (2010). The effects of 2,4-dichlorophenoxy acetic acid and isoproturon herbicides on the mitotic activity of wheat (Triticum aestivum L.) root tips. Turkish Journal of Biology, 34 (1), 55–66.
  18. West, G., Inze, D., Beemster, G. (2004). Cell Cycle Modulation in the Response of the Primary Root of Arabidopsis to Salt Stress. Plant Physiology, 135 (2), 1050–1058. doi: 10.1104/pp.104.040022
  19. V. Ya. Yuryev Рlant Production Institute of NAAS. Available at: http://www.yuriev.com.ua/index.php?lang=ua
  20. Baryikina, R. P., Veselova, T. D., Devyatov, A. G., Dzhalilova, H. H., Ilina, G. M., Chubatova, N. V. (2004). Spravochnik po botanicheskoy mikrotehnike. Osnovyi i metodyi [Handbook botanical microtechnology. Fundamentals and Methods]. Moscow: Publishing house Moscow State University, 312.
  21. Atramentova, L. A., Utevskaya, O. M. (2008). Statisticheskie metodyi v biologii [Statistical Methods in Biology]. Gorlovka: Lihtar, 248.

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Published

2017-04-26

How to Cite

Avksentiievа O., & Shulik, V. (2017). Research of influence of contrasting trophic conditions of vernalization on the mitotic activity of meristems, growth and development of winter wheat. ScienceRise: Biological Science, (2 (5), 4–9. https://doi.org/10.15587/2519-8025.2017.99664

Issue

No. 2 (5) (2017)

Section

Biological Sciences

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Copyright (c) 2017 Olga Avksentiievа, Victoria Shulik

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