Prognostic assessment of cryosensitivity level for umbilical cord blood hemopoietic tissue with the help of markers of prooxidant activity processes

Authors

  • Tetiana Kalynychenko State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine" Maksyma Berlynskoho str., 12, Kiyv, Ukraine, 04060, Ukraine https://orcid.org/0000-0002-4905-3256

DOI:

https://doi.org/10.15587/2519-4798.2018.138222

Keywords:

umbilical cord blood hemopoietic tissue, cryosensitivity, lipid peroxidation, granulocyte-macrophage hemopoietic progenitor cells, low-temperature cord blood banks

Abstract

Aim. To investigate the connection between the levels of human umbilical cord blood hemopoietic tissue (UCB HT) cryosensitivity (using of the granulocyte-macrophage progenitors (GMPs) percentage loss) and activity of prooxidant processes in whole blood before the beginning of its cryopreservation.

Methods of research. Cryopreservation of UCB nuclear cell fraction was carried out by slow freezing under protection of the dimethyl sulfoxide in 5% final concentration (v/v). The loss of GMPs was determined by the difference in the total content of colonies and clusters prior to cryopreservation and after thawing the sample in a short-term tissue culture. The activity of prooxidant processes in UCB was studied by biochemical markers of lipid peroxidation products (LPO) using a spectrophotometric method for determining the concentrations of such products as substrates for lipid peroxidation (isolated double bonds - IDB), intermediate (conjugated dienes (CD), conjugated trienes (CT), conjugated oxodienes (COD)) and final LPO products such as Schiff bases (ShB) for neutral lipids and phospholipids. Data analysis performed on models of analytical groups, regression analysis, mutual conjugation.

Results. It has been demonstrated that the level of UCB HT cryosensitivity (due to loss of GMPs) has direct correlation with LPO activity indices (from medium to high significance level). The relative risk (RR) of loss of GMPs is significantly increased under the condition of high levels of LPO phospholipid peroxidation in UCB before beginning cryopreservation. In particular, in the case of phospholipid peroxidation, it is: for IDB - RR = 5.29; 95% CI: 2.69-10.38; p <0.001; CD - RR = 5.73; 95% CI: 2.88-11.40; p <0.001; CT and COD - RR = 2.81; 95% CI: 1.72-4.60; p <0.001; ShB - RR = 1, 92, 95% CI: 1.16-3.18, p <0.01, respectively.

Conclusions. Evaluation of the activity level of prooxidant processes in UCB using the biochemical markers before the start of the freezing procedure is valuable because of the possibility of creating an early prediction of the HT cryosensitivity, which can be useful in choosing cryopreservation tactics

Author Biography

Tetiana Kalynychenko, State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine" Maksyma Berlynskoho str., 12, Kiyv, Ukraine, 04060

PhD, Senior Researcher, Head of Laboratory

Laboratory of Hemopoietic Cell Cryopreservation

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Published

2018-07-19

How to Cite

Kalynychenko, T. (2018). Prognostic assessment of cryosensitivity level for umbilical cord blood hemopoietic tissue with the help of markers of prooxidant activity processes. ScienceRise: Medical Science, (5 (25), 52–58. https://doi.org/10.15587/2519-4798.2018.138222

Issue

No. 5 (25) (2018)

Section

Medical Science

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Copyright (c) 2018 Tetiana Kalynychenko

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