Clostridium perfringens: characterization, biological activity, the indication in food

Authors

DOI:

https://doi.org/10.15587/2312-8372.2015.39107

Keywords:

Clostridium perfringens, description, biological action, PCR-determination, food products, accelerated method

Abstract

The main properties of the microorganisms C. perfringens, their stability, ability to preserve in raw material and products of its processing have been characterized. The biological activity of C. perfringens, its ability to cause the food poisoning of people, spoilage of food products have also been described. These clostridia are widely distributed in the environment that leads to contamination of raw material and food products by means of them. In this regard, C. perfringens is regulated in raw materials and food products, it is not allowed in can food with a рН above 3,5 and this microorganizm should be systematically controlled. C. perfringens as the microorganism-contaminant is a criterion of sanitary safety of products. The classic and modern methods of determination of C. perfringens have been described. It has been shown that the basic methods of identification of this microorganism in food products are either prolonged or insufficiently specific. The results of development of indicative accelerated method for determination of C. perfringens in food products using polymerase chain reaction (PCR) have been presented. Species-specificity of the developed method has been confirmed. The developed PCR-method allows to make determination of C. perfingens ten times as quick in comparison with the traditional methods of research, to get a reliable high-qualitive or quantitative result and describe the regulated degree of sanitary safety of products, that is especially important for perishable raw materials and the long-term storage ones. 

Author Biography

Инна Васильевна Пилипенко, Odessa National Academy of Food Technologies, str. Kanatnaya, 112, Odessa, Ukraine, 65039

Candidate of Technical Sciences, Associate Professor

Department of Biotechnology, Canned Products and Drinks

References

  1. Adcock, P. W., Saint, C. P. (2001, September 1). Rapid Confirmation of Clostridium perfringens by Using Chromogenic and Fluorogenic Substrates. Applied and Environmental Microbiology, Vol. 67, № 9, 4382–4384. doi:10.1128/aem.67.9.4382-4384.2001
  2. McClane, B. A. (2001, November). The complex interactions between Clostridium perfringens enterotoxin and epithelial tight junctions. Toxicon, Vol. 39, № 11, 1781–1791. doi:10.1016/s0041-0101(01)00164-7
  3. Persianova, I. P., Gerasimenko, L. N., Stoyanova, L. A. (2010). Microbiologiya conservirovaniy i microbiologicheskiy control conservnogo proizvodstva [Microbiology of canning and microbiological control of canning production]. Odessa, Ukraine:”Vneshreklamservis”, 310.
  4. In: Mazohina-Porshnyakova, N. N. (1977). Аnаliz i otsenka kachestva konservov po microbiologicheskim pokazatelyam [Analysis and evaluation of the quality of canned food for microbiological indicators]. Moscow: Food Industry, 471.
  5. In: Blackburn, Clive de W. (2008). Mikrobiologicheskaya porcha pichevih productov [Food spoilage microorganisms]. C-Pb : Profession, 784.
  6. Melngaile, A.; Latvia University of Agriculture. (2008). Microbiological risk analysis in public catering establishments. Jelgava, 68.
  7. Pylypenko, L. N., Verhivker, Y. G., Pylypenko, I. V. (2015). Коnservirovanie pischevih productov. Мicrobiologiya, energetica, cоntrol [Canning food. Microbiology, power, control]. Оdеssа, Ukraine: «ВМВ», 232.
  8. De Jong, A. E. I., Eijhusen, G. P., Brouwer-Post, E. J. F., Grand, M., Johansson, T., Kärkkäinen, T., Marugg, J., et al. (2003, September). Comparison of media for enumeration of Clostridium perfringens from foods. Journal of Microbiological Methods, Vol. 54, № 3, 359–366. doi:10.1016/s0167-7012(03)00069-1
  9. Jose, S. G.-A., Labbe, R. G., Manuel, A. R. (1992). Sporulation and enterotoxin production by Clostridium perfringens Type A at 37 and 43 оC. Applied and Environmental Microbiology, Vol. 58, № 4, 1411–1414.
  10. Instruktsiya № І 4.4.4.077 pro poryadok sanitarno-tehnicheskogo controlya konservov na virobnichih pidpriemstvah. (2001). [Instruction № І 4.4.4.077 on the procedure of sanitary control at industrial enterprises]. Resolution of the Chief State Sanitary Doctor of Ukraine from 07.11.2001 № 140. Available: http://www.uazakon.com/big/text694/pg1.htm. Last accessed 01.02.2015.
  11. SanPiN 2.3.2.1078-01. Gigienicheskie trebovaniya bezopasnosti i pischevoy tsennosti pischevih productov. (2002). [Hygienic requirements for safety and nutrition value of food products. Sanitary-epidemiological rules and norms SanRaN 2.3.2.1078-01]. Introduced from 01.09.2002. Available: http://blanker.ru/doc/sanpin-2-3-2-1078-01. Last accessed 04.02.2015.
  12. GOST 29185-91. Producti pischevie. Metodi viyavleniya i opredeleniya kolichestva sulfitredutsiruyuschih klostridiy. (1991). [State Standart 29185-91. Food products. Methods for detection and determination of the quantity of sulphite-reducing clostridia]. Available: http://vsegost.com/Catalog/19/1911.shtml. Last accessed 01.02.2015.
  13. Rodrigues, U. M., Kroll, R. G. (1988, January). Rapid selective enumeration of bacteria in foods using a microcolony epifluorescence microscopy technique. Journal of Applied Bacteriology, Vol. 64, № 1, 65–78. doi:10.1111/j.1365-2672.1988.tb02430.x
  14. Wang, X., Yamaguchi, N., Someya, T., Nasu, M. (2007, October). Rapid and automated enumeration of viable bacteria in compost using a micro-colony auto counting system. Journal of Microbiological Methods, Vol. 71, № 1, 1–6. doi:10.1016/j.mimet.2007.06.019
  15. МR 02.011-06. Uskorennie metodi viyavleniya sanitarno-pokazatelnih i patogennih microorganizmov s ispolzovaniem podlozhek "RIDA(R)COUNT", Chisso Corporation, Yaponiya. (2006). [Methodical Management 02.011-06. Accelerated methods for identifying sanitary-indicative and pathogenic microorganisms using substrates "RIDA(R)COUNT", produced by Chisso Corporation, Japan]. Available: http://www.fsetan.ru/library/doc/mr-02011-06-uskorennyie-metodyi-vyiyavleniya-sanitarno-pokazatelnyih-i-patogennyih-mikroorganizmov-s-ispolzovaniem-podlozhek-ridarcount-proizvodstva-chisso-corporation-yaponiya/. Last accessed 05.02.2015.
  16. Satina, O. I. Quick and rapid methods for microbiological analysis. Available: http://www.vniipp.ru/s11.pdf. Last accessed 07.02.2015.
  17. Shimizu, S., Ootsubo, M., Kubosawa, Y., Fuchizawa, I., Kawai, Y., Yamazaki, K. (2009, June). Fluorescent in situ hybridization in combination with filter cultivation (FISHFC) method for specific detection and enumeration of viable Clostridium perfringens. Food Microbiology, Vol. 26, № 4, 425–431. doi:10.1016/j.fm.2009.02.002

Published

2015-04-02

How to Cite

Пилипенко, И. В. (2015). Clostridium perfringens: characterization, biological activity, the indication in food. Technology Audit and Production Reserves, 2(4(22), 4–8. https://doi.org/10.15587/2312-8372.2015.39107

Issue

Section

Technologies of food, light and chemical industry