The influence of cultivation conditions on biosynthetic ability of Eremothecium ashbyi Guillierm.

Authors

  • Валентина Юріївна Поліщук National Technical University of Ukraine «Kyiv Polytechnic Institute», Prospect Peremogy, 37, Kyiv, Ukraine, 03056, Ukraine https://orcid.org/0000-0002-1284-584X
  • Олексій Мартем’янович Дуган National Technical University of Ukraine «Kyiv Polytechnic Institute», Prospect Peremogy, 37, Kyiv, Ukraine, 03056, Ukraine https://orcid.org/0000-0001-5594-6088

DOI:

https://doi.org/10.15587/2312-8372.2016.65470

Keywords:

Eremothecium ashbyi, riboflavin producer, submerged cultivation, physiological and biochemical properties

Abstract

Despite the extremely poor riboflavin amount in the food for people and animals and its important role in the biochemical processes of metabolism, yet little attention is paid to the production of vitamin B2 by biotechnological means. Therefore, the main aim is to study an impact of physical and chemical parameters of fermentation and various power sources of ascomycetes Eremotecium ashbyi to producing the desired product.

The main biosynthetic indicators of E.ashbyi culture, which were defined in the fermentation process, are filamentous biomass and riboflavin. In this work an optimal pH values for the best accumulating biomass and riboflavin environment is defined and positive effects on increasing the intensity of mixing on yield of the desired product are shown. For biomass accumulation it is recommended to create a nutrient medium with pH 5,5-6,0 and use low mixing level. To maximize riboflavin biosynthesis is necessary pH 7,5 and intensive mixing.

For the first time shows the rate of riboflavin synthesis by E.ashbyi strain depending on sources of carbon and nitrogen supply, which is especially important for the further improvement of production technology of riboflavin. The best results for riboflavin synthesis were shown to sorbitol, fructose, galactose and methionine, arginine, peptone. The most promising complex environment is unhopped beer wort.

Author Biographies

Валентина Юріївна Поліщук, National Technical University of Ukraine «Kyiv Polytechnic Institute», Prospect Peremogy, 37, Kyiv, Ukraine, 03056

Assistant

Department of industrial biotechnology 

Олексій Мартем’янович Дуган, National Technical University of Ukraine «Kyiv Polytechnic Institute», Prospect Peremogy, 37, Kyiv, Ukraine, 03056

Doctor of Biological Sciences, Professor

Department of industrial biotechnology 

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Published

2016-03-29

How to Cite

Поліщук, В. Ю., & Дуган, О. М. (2016). The influence of cultivation conditions on biosynthetic ability of Eremothecium ashbyi Guillierm. Technology Audit and Production Reserves, 2(4(28), 35–41. https://doi.org/10.15587/2312-8372.2016.65470

Issue

Vol. 2 No. 4(28) (2016): Technologies of food, light and chemical industry

Section

Technologies of food, light and chemical industry

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Copyright (c) 2016 Валентина Юріївна Поліщук, Олексій Мартем’янович Дуган

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