Obtaining of highly active streptomycin-resistant bacteriolysins’s producers of Streptomyces albus

Authors

DOI:

https://doi.org/10.15587/2312-8372.2015.40552

Keywords:

Streptomyces albus 2435, superproducer, bacteryolysins, selection, nitrozoguanidin, streptomycin

Abstract

The application of N-methyl-N'-nitro-N-nitrozoguanidin (NG), as chemical mutagen along with the use of mutations of resistance to an antibiotic streptomycin in the selection of a producer of the bacteryolytic enzyme complex of Streptomyces albus 2435 with the increased biosynthetic ability  are investigated. The conditions of NG mutagen treatment (1 mg/ml, 20 min.), allowing to obtain mutants with supersynthesis of a target product are determined.

The nitrozoguanidin-induced strains of Streptomycesalbus105 and 107, steadyto streptomycin (in concentration of 100-250 mkg/ml) with the increased (1,6 times) abilityto synthesis of bacteryolysins are received. The efficiency of the offered combination of selective factors (nitrozoguanidin  and mutation of streptomycin-resistance) for the selection of producers in the course of the supporting selection and for industrial application is shown.

The need of a producer’s supporting selection is caused by heterogeneity of cultures of streptomycetes and decrease in biosynthetic activity in the course of storage. For the development of industrial technology only the producer with supersynthesis of a product, in this case a producer of antimicrobial enzyme  preparations, can be used.

Author Biographies

Олександр Миколайович Громико, Ivan Franko National University of Lviv, Hrushvskoho str., 4, Lviv, Ukraine, 79005

Candidate of Biological Sciences, Senior Researcher

Department of genetics and biotechnology

Андрій Васильович Буцяк, Ivan Franko National University of Lviv, Hrushvskoho str., 4, Lviv, Ukraine, 79005

PhD-student

Department of genetics and biotechnology

Віктор Олександрович Федоренко, Ivan Franko National University of Lviv, Hrushvskoho str., 4, Lviv, Ukraine, 79005

Doctor of Biological Sciences, Professor, Head of the Department

Department of genetics and biotechnology

Тетяна Сергіївна Тодосійчук, National Technical University of Ukraine “Kyiv Polytechnic Institute”, Peremogy av., 37, Kiev, Ukraine, 03056

Candidate of Technical Sciences, Assistant Professor, Head of the Department

Department of industrial biotechnology

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Published

2015-04-02

How to Cite

Громико, О. М., Буцяк, А. В., Федоренко, В. О., & Тодосійчук, Т. С. (2015). Obtaining of highly active streptomycin-resistant bacteriolysins’s producers of Streptomyces albus. Technology Audit and Production Reserves, 2(4(22), 44–50. https://doi.org/10.15587/2312-8372.2015.40552

Issue

Vol. 2 No. 4(22) (2015): Technologies of food, light and chemical industry

Section

Technologies of food, light and chemical industry

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Copyright (c) 2016 Олександр Миколайович Громико, Андрій Васильович Буцяк, Віктор Олександрович Федоренко, Тетяна Сергіївна Тодосійчук

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