Pharmacological study of original extracts of corn silk

Authors

DOI:

https://doi.org/10.15587/2519-8025.2022.271049

Keywords:

corn silk extracts, lipid peroxidation, antioxidant activity, choleretic activity, acute toxicity, Drosophila melanogaster Meig. test system, hepatoprotectors\

Abstract

Aim: the aim of the research is a pharmacological study of the original extracts of corn silk.

Materials and methods. The object of the study were 4 original extracts of corn silk, obtained by extraction with water and ethanol of different concentrations, namely: aqueous extract of corn silk (ACSE), extract of corn silk, extracted with ethanol 30 % (ECSE 30 %) , extract of corn silk, extracted with ethanol 50 % (ЕCSЕ 50 %), extract of corn silk, extracted with ethanol 70 % (ЕCSЕ 70 %). A screening study of the antioxidant properties of corn silk extracts was carried out in vitro using models of spontaneous and ascorbate-induced lipid peroxidation (LPO) in rat liver homogenate.

Results. As can be seen from the given data, ECSE 50 % at a dose of 20 mg/kg showed a pronounced protective effect on the hepatotoxicity of TСM. Its use led to a probable restoration of the bile-forming function of the liver against the background of tetrachloromethane hepatitis: in response to an increase in the cholesterol content (by 43 %, p<0.05), the content of bile acids increased 2 times (p<0.05), as a result of which cholate -cholesterol ratio approached the level of indicators of intact animals, the rate of bile secretion normalized. Based on the analysis of the results of the study, it can be concluded, that in terms of bile-forming and choleretic activity on the tetrachloromethane hepatitis model, ECSE 50 % was not inferior to silibor and superior to quercetin (р<0.05).

Conclusions. Biologically active substances of corn silk affect not only the diffusion and filtration processes of the liver parenchyma, but also the biosynthesis and transport of its organic components, that is, they affect the bile-forming function

Author Biographies

Olga Naboka, National University of Pharmacy

Doctor of Biological Sciences, Professor

Department of Biochemistry

Liliia Vyshnevska, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Technology of Drugs

Olga Filiptsova, National University of Pharmacy

Doctor of Biological Sciences, Professor

Department of Cosmetology and Aromology

Oksana Tkachenko, National University of Pharmacy

PhD, Associate Professor

Department of Biological Chemistry

Olha Vislous, National University of Pharmacy

PhD, Assistant

Department of Medical Chemistry

References

  1. Naboka, O. I., Khouari, S., Glushchenko, A. V., Georgiyants, V. A. (2014). Antioxidant properties of extracts of aerial part of Bupleurum aureum, hill-growing saltwort herb, Fumaria schleicheri and Cynara scolymus in vitro and in vivo. Journal of Chemical and Pharmaceutical Research, 6 (7), 172–177.
  2. Kulkarni, S. R., Ravindra, K. P., Dhume, C. Y., Rataboli, P., Rodrigues, E. (2019). Levels of plasma testosterone, antioxidants and oxidative stress in alcoholic patients attending deaddiction centre. Biology and Medicine, 1 (4), 11–20.
  3. Manach, C., Scalbert, A., Morand, C., Rémésy, C., Jiménez, L. (2004). Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition, 79 (5), 727–747. doi: https://doi.org/10.1093/ajcn/79.5.727
  4. Baraboi, V. A., Sutkovoi, D. A.; Zozuli, Iu. A. (Ed.) (1997). Okislitelno-antioksidantnyi gomeostaz v norme i patologii. Kyiv: Chernobylinterinform, 257.
  5. Tkachenko, M. F., Kovalov, V. M., Kononenko, A. G., Maloshtan, L. M. (2009). Vivchennia flavonoidnogo skladu listkіv kukurudzi su-1. Zbіrnik naukovikh pratc spіvrobіtnikіv NMAPO іm. P. L. Shupika, 18, 497–502.
  6. Vladimirov, Iu. A., Archakov, A. I. (1972). Perekisnoe okislenie lipidov v biologicheskikh membranakh. Moscow: Nauka, 252.
  7. Khouari, S., Naboka, O. I. (2016). Toxicity of Bupleurum Aureum extracts in Drosophila melanogaster Meig. Study. Topical issues of new drugs development. Kharkiv: NUPh, 20.
  8. Shkorbatov, Yu. H., Pasiuha, V. N., Shakyna, L. A., Hrabyna, V. A. et al. (2007). Vlyianye mahnytnoho polia y mykrovolnovoho yzluchenyia na zhyznesposobnost drozofyli. Dosiahnennia i problemy henetyky, selektsii ta biotekhnolohii. Zbirnyk naukovykh prats. Vol. 1. Kyiv: Lohos, 334–338.
  9. Shkorbatov, Yu. H., Shakina, L. O., Pasiuha, V. M., Rybalchenko, T. V. (2010). Doslidzhennia vplyvu riznometalichnykh kompleksnykh spoluk na stan khromatynu v klitynakh liudyny i zhyttiezdatnist drozofily. Problemy ekolohichnoi ta medychnoi henetyky i klinichnoi imunolohii. Kyiv-Luhansk, 4, 354–364.
  10. Zhuravlova, L., Strashniuk, V., Shakhbazov, V. (2004). Vplyv shchilnosti kultury na proiav efektu heterozysu u Drosophila melanogaster. Visnyk Lvivskoho universytetu. Biolohichna seriia, 35, 102–109.
  11. Marchyshyn, S. M., Kovalova, Ye. O., Berdei, T. S. (2013). Vyvchennia zhovchohinnoi dii sukhoho ekstraktu travy chornobryvtsiv. Naukovo-tekhnichnyi prohres i optymizatsiia tekhnolohichnykh protsesiv stvorennia likarskykh preparativ. Ternopil: Ukrmedknyha, 373.
  12. Arutiunian, A. V., Dubynyna, E. E., Zibyna, N. N. (2000). Metodi otsenky svobodnoradykalnoho okyslenyia y antyoksydantnoi systemi orhanyzma. Saint Petersburg: YKF «Folyant», 104.
  13. Drohovoz, S. M., Hubskyi, Yu. I., Skakun, M. P., Kovalenko, V. M., Derymedvid, L. V.; Stefanov, O. V. (Ed.) (2001). Eksperymentalne vyvchennia zhovchohinnoi, kholelitiaznoi ta hepato-protektornoi aktyvnosti novykh likarskykh zasobiv. Doklinichni doslidzhennia likarskykh zasobiv. Kyiv, 334–351.
  14. Kamyshnikov, V. S. (Ed.) (2013). Metody klinicheskikh laboratornykh issledovanii. Moscow: MEDpress-in form, 736.
  15. European convention for the protection of vertebrate animals used for experimental and other scientific purpose (1986). Council of Europe 18.03.1986. Strasbourg, 52.
  16. Lapach, S. N., Chubenko, A. V., Babich, P. N. (2001). Statisticheskie metody v mediko-biologicheskikh issledovaniiakh s ispolzovaniem Excel. Kyiv: Morin, 320.
  17. Kononenko, A. G., Maloshtan, L. M., Tkachenko, M. F., Kovalov, V. M. (2009). Vivchennia gepatoprotektornoi aktivnostі ekstraktu listkіv kukurudzi v umovakh gostrogo tetrakhlormetanovogo gepatitu. Zaporozhskii meditcinskii zhurnal, 11 (5), 115–117.
  18. Shkarina, E. I., Maksimova, T. V., Nikulina, I. N. et al. (2008). O vliianii biologicheski aktivnikh veshchestv na antioksidantnuiu aktivnost fitoprep-reparatov. Khimiko-farmatcevticheskii zhurnal, 35 (6), 40–47.
  19. Kurkin, V. A. (2002). The flavonoids as the bioantioxidants. Polyphenols Communications 2002. Morocco, 285–286.
  20. Sidorov, K. K. (1973). O klassifikatcii toksichnosti iadov pri parenteralnykh sposobakh vvedeniia. Toksikologiia novykh promyshl. khim. v-v. Moscow: Meditcina, 47–51.
  21. Strashniuk, V. Yu., Shakina, L. O., Skorobahatko, D. O. (2014). Pat. No. 90336 UA. Sposib vyznachennia henotoksychnoi dii khimichnoho abo fizychnoho chynnyka. MPK (2013.01) G01N 33/554. № u201313929; declareted: 02.12.2013; published: 26.05.2014, Bul. No. 10, 4.
  22. Otsenka mutahennosty novikh lekarstvennikh sredstv. Moscow, 20.
  23. Rukovodstvo po kratkosrochnym testam dlia vyiavleniia mutagennykh i kantcerogennykh khimicheskikh veshchestv. Gigienicheskie kriterii sostoianiia okruzhaiushchei sredy (1989). Vsemirnaia organizatciia zdravookhraneniia. Zheneva, 212.

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Published

2022-12-30

How to Cite

Naboka, O., Vyshnevska, L., Pasynchuk, I., Filiptsova, O., Tkachenko, O., & Vislous, O. (2022). Pharmacological study of original extracts of corn silk. ScienceRise: Biological Science, (4(33), 10–17. https://doi.org/10.15587/2519-8025.2022.271049

Issue

No. 4(33) (2022)

Section

Biological Sciences

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Copyright (c) 2023 Olga Naboka, Liliia Vyshnevska, Oksana Tkachenko, Inna Pasynchuk, Olha Vislous

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