DOI: https://doi.org/10.15587/2519-4852.2018.141412

The research of free aminoacids of water-soluble protein-polysaccharide complex of oyster mushroom pleurotus ostreatus

Boris Varinsky, Nataliia Kucherenko, Olga Kolpakova

Abstract


Recently, the share of medicinal products of natural origin has increased in the pharmaceutical market of Ukraine. This is due to the fact that the pharmacological effect of natural substances provides a complex of biologically active substances, which has a high therapeutic effect and the minimum number of adverse reactions. Therefore, the study of chemical composition and standardization of natural substances is an important stage in pharmaceutical research.

A promising source of active ingredients is the mushroom Pleurotus ostreatus. The chemical composition of this fungus is represented by a large number of biologically active substances, which ensures its many-sided use in folk medicine.

Aim. The determination of qualitative and quantitative composition of free amino acids in the composition of water-soluble protein-polysaccharide complex (WPPSC) of oyster mushroom Pleurotus ostreatus was the aim of research.

Methods. Physicochemical methods are the most commonly used to study active substances of natural origin. This is due to the fact that they provide high informative, accurate, efficient and reproducible. The method of high performance liquid chromatography (HPLC) was used to analyze the amino acid composition of the WPPSC of oyster mushroom Pleurotus ostreatus. This method is based on the separation of individual components due to different adsorption capacity.

Results. Research of the amino acid composition of the WPPSC of oyster mushroom Pleurotus ostreatus showed that the complex contains 10 (7 %) free amino acids, among which 5 are essential. The detected aminoacids are aliphatic, heterocyclic and aromatic compounds. They are presented by glycine, alanine, leucine, isoleucine, threonine, selinum, lysine, arginine, histidine and phenylalanine.

Conclusion. The qualitative and quantitative composition of the free amino acids of the WPPSC of oyster mushroom Pleurotus ostreatus was established. The method of determination and separation of free amino acids by the method of high-performance liquid chromatography is proposed. The obtained results are proposed for standardization of the initial substance in the process of production of medicines based on WPPSC of oyster mushroom Pleurotus ostreatus


Keywords


Pleurotus ostreatus; biologically active substances; amino acids; high performance liquid chromatography; standardization

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References


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GOST Style Citations


Solodovnychenko N. M., Zhuravlov M. S., Kovalov V. M. Likarska roslynna syrovyna ta fitopreparaty: pos. Kharkiv: Vyd-vo NFAU: Zoloti storinky, 2001. 408 p.

Analiticheskaya himiya v sozdanii, standartizacii i kontrole kachestva lekarstvennyh sredstv. Vol. 2: monografiya / V. P. Georgievskiy (Ed.). Kharkiv: «NTMT», 2011. 474 p.

Molitorus H. P. Mushrooms in medicine // Folia Microbiol. 1994. Vol. 39, Issue 2. P. 91–98.

Mariappan S., Vinayagam S., Durai M. Phytochemical screening of bioactive compounds from Pleurotus ostreatus (jacq.fr) kumm-an wild edible mushroom // World Journal of Pharmaceutical Research. 2015. Vol. 4, Issue 5. Р. 1603–1618.

Mowsurni F., Chowdhury M. Oyster Mushroom: Biochemical and Medicinal Prospects // Bangladesh Journal of Medical Biochemistry. 2013. Vol. 3, Issue 1. Р. 23–28. doi: https://doi.org/10.3329/bjmb.v3i1.13804 

TU U 10.8-02010675-001:2017 Kompleks bilkovo-polisakharydnyi.

Kucherenko N. V., Martynov A. V., Demianenko V. H. Rozrobka metodyk standartyzatsiyi vodorozchynnoho bilkovo-polisakharydnoho kompleksu, otrymanoho z hryba Pleurotus Ostreatus // Farmatsevtychnyi zhurnal. 2008. Issue 1. P. 92–95.

Minaieva V. O. Khromatohrafichnyi analiz: pidr. Cherkasy, 2013. 284 p.

Peptide and amino acids separation and identification from natural products / Neda I. et. al. // Analytical Chemistry. 2012. Issue 6. Р. 135–146. doi: https://doi.org/10.5772/51619 

Konovalova E. Yu., Stazhyla E. N., Lebeda A. Ph. Amino acid research of family elaeagnaceae juss. Plants' leaves // Fitoterapiya. 2010. Issue 2. P. 60–64.

Determination of amino acids in medicinal plants from Southern Sonora, Mexico / Moran-Palacio E. J. et. al. // Tropical Journal of Pharmaceutical Research. 2014. Vol. 13, Issue 4. Р. 601–606. doi: https://doi.org/10.4314/tjpr.v13i4.17 

Sputnik hromatografista. Metody zhidkostnoy hromatografii / Rudakov O. B. et. al. Voronezh: Vodoley, 2004. 528 p.

Minazova G. I. Highly effective liquid chromatography in the analysis of natural raw material // Bashkirskiy himicheskiy zhurnal. 2010. Vol. 17, Issue 4. P. 134–136.

Aminokisloty glazami himikov, farmacevtov, biologov. Vol. 1 / Syrovaya A. O. et. al. Kharkiv: Shchedra sadiba plyus, 2014. 228 p.

Davies J. S. Amino Acids, Peptides and Proteins. Cambridge: The Royal Society of Chemistry, 2006. 472 p.







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