DOI: https://doi.org/10.15587/2519-4852.2019.173459

Biologically active substances of salix purpurea F. Gracilis (Gren. & Godr.) C.K. Schneid. (Salicaceae)

Natalia Borodina, Volodimir Kovalyov, Oleh Koshovyi

Abstract


The medicinal raw materials of many representatives of the Salicaceae family have long been widely used both in folk medicine and in modern pharmaceuticals. Currently, some species of willow are official medicinal raw materials in some European countries. In 2014, "Salicis cortex" acquired the status of official medicinal raw material and included in the State Pharmacopoeia of Ukraine. In this regard, the study of biologically active compounds of different species, varieties and hybrid forms of willow is important, which will allow to expand the range of medicinal plant material both at the expense of local and at the expense of introduced species of willow, common in Ukraine.

Aim. Study of qualitative and quantitative composition of biologically active substances of Salix purpurea f. Gracilis (Gren. & Godr.) C.K. Schneid., Growing in Ukraine.

Methods of the research. The object of the study were dry shoots of the Salix purpurea f. Gracilis (Gren. & Godr.) C.K. Schneid. Plant raw materials were collected in 2016–2017 years by the NBS named after M. M. Grishko National Academy of Sciences of Ukraine. The component composition of volatile substances was determined using the Agilent Technologies 6890 chromatograph with a mass spectrometer detector 5973. The content of the phenolic substances was determined colorimetrically by the Folin-Ciocalteau method. Component composition of phenolic substances was determined by high performance liquid chromatography (HPLC) using the Prominence LC-20 Liquid Chromatographic System Shimadzu (Japan).

Results. The qualitative composition and quantitative content of volatile compounds and phenolic substances of Salix purpurea f. Gracilis (Gren. & Godr.) C.K. Schneid shoots have been determined. It was established that the raw material contains rather high concentrations of volatile compounds, which are dominated by aromatic - in particular, geraniol and eugenol, among the terpenoids, squalene predominates. It was found that phenolic substances are represented by flavonoids and hydroxycinnamic acids. Among substances of phenolic nature dominated by flavanones. The conducted studies confirm the feasibility of further studies of willow species.

Conclusions. The qualitative composition and quantitative content of volatile compounds and phenolic substances in Salix purpurea f. Gracilis (Gren. & Godr.) C.K. Schneid shoots have been determined. The studies significantly expanded the data on the chemical composition of raw materials of plants of the genus Salix L. Data obtained from the study of Salix purpurea f. Gracilis (Gren. & Godr.) C.K. Schneid will be used for the planning of pharmacological research and development of QCM for raw materials and medicines

Keywords


Salicaceae; willow; shoots; biologically active substances; volatile compounds; phenolic substances

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References


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GOST Style Citations


Borodina N. V. Research of volatile compounds from Salix cinerea L // // Oxford Review of Education and Science. 2016. Vol. V, Issue 1 (11). Р. 468–473.

Derzhavna Farmakopeia Ukrainy. Vol. 1. Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 2015. 1128 p.

Dendroflora Ukrainy. Dykorosli y kultyvovani dereva i kushchi. Pokrytonasinni. Chastyna I. Dovidnyk / Kokhno M. A., Parkhomenko L. I., Zarubenko A. U. et. al.; ed. by Kokhno M. A. Kyiv: Fitosotsiotsentr, 2002. 448 p.

Fuchylo Ya. D., Sbytna M. V. Vepby Ukpainy (biolohiia, ekolohiia, vykopystannia): monograph. Kyiv: Lohos, 2009. 200 p.

Checklist for cultivars of Salix L. (willow) International Salix Cultivar Registration Authority FAO. International Poplar Commission, 2015. 163 р.

Chen-Fu F., Skvortsov A. K. Validation of Hao’s new Chinese taxa in Salix (Salicaceae) // Novon. 1998. Vol. 8, Issue 4. P. 467–470. doi: http://doi.org/10.2307/3391877

Enerhetychna verba: tekhnolohiia vyroshchuvannia ta vykorystannia / Roik M. V., Sinchenko V. M., Fuchylo Ya. D. et. al.; ed. by Sinchenko V. M. Vinnytsia: TOV «Nilan – LTD», 2015. 340 p.

Derzhavna Farmakopeia Ukrainy. Vol. 3. Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 2014. 732 p.

European Pharmacopoeia. Council of Europe, European Department for the Council of Medicines. Strasbourg, 2005.

Kodakova M. N., Dubishhev A. V. Sravnitelnaia otsenka farmakologicheskogo effekta rastitelnykh preparatov semeistva ivovykh // Meditsinskii vestnik Bashkortostana. 2009. Vol. 4, Issue 2. P. 193–196.

Frolova O. O., Kompantseva E. V., Dementieva T. M. Biologically Active Substances of Plants from Sali x L. Genus // Pharmacy & Pharmacology. 2016. Vol. 4, Issue 2 (15). P. 41–59. doi: http://doi.org/10.19163/2307-9266-2016-4-2(15)-41-59

Zhebrak I. S., Tsybulko E. V. Antimikrobnaia aktivnost vodnykh nastoev kory Salix purpurea L: proceeding // Aktualnye problemy ekologii. Grodno: GrGU, 2010. P. 35–37.

Rasteniia semeistva ivovykh – perspektivnyi istochnik novykh antimikrobnykh, protivovospalitelnykh i toniziruiushhikh lekarstvennykh sredstv: proceeding / Kurkin V. A. et. al. // Poisk, razrabotka i vnedrenie novykh lekarstvennykh sredstv i organizatsionnykh form farmatsevticheskoi deiatelnosti. Tomsk, 2000. P. 42–43.

Borodina N. V. Izuchenie letuchikh komponentov Salix caprea L // Proceedings of 4-th European Conference on Biology and Medical Sciences. Vienna, 2015. Р. 209–213.

Borodina N. V., Kovalev V. N., Koshevoi O. N. Sravnitelnyi analiz aminokislotnogo sostava pobegov Salix purpurea L., Salix viminalis L., Salix fragilis L // Vestnik Iuzhno-Kazakhstanskoi gosudarstvennoi farmatsevticheskoi akademii. 2014. Vol. 4, Issue 3 (68). P. 53–55.

Borodina N. V., Kovalev V. N. Sravnitelnyi analiz fenolnykh soedinenii pobegov Salix caprea L., Salix purpurea L., Salix viminalis L. flory Ukrainy: proceeding // Fenolnye soedineniia: fundamentalnye i prikladnye aspekty. Moscow, 2015. P. 27–33.

Borodina N. V. Fenolni spoluky Salix matsudana Koidz: proceeding // Vesniani naukovi chytannia. Kyiv: Tsentr naukovykh publikatsii, 2017. P. 129–136.

Borodina N. V. Khromato-mass-spektrometrycheskoe yzuchenye lystev Salix caprea L: proceeding // Aktualni pytannia rozvytku medychnykh nauk u XXI st. Lviv: Lvivska medychna spilnota, 2015. P. 106–108.

The study of Salix myrsinifolia Salisb. leaves by the GC/MS method / Borodina N. V., Kovalyov V. N., Koshovyi О. N., Stremoukhov A. A. // The 9th International Conference on Pharmaceutical Sciences and Pharmacy Practice, dedicated to the 100th years anniversary of independent Lithuania's pharmacy. Kaunas: LSMU, 2018. Р. 105.

Borodina N. V., Kovalov V. M. Khromato-mas-spektrometrychne doslidzhennia syrovyny ta lipofilnoho ekstraktu Salix alatavica: proceeding // Teoretychni ta praktychni aspekty doslidzhennia likarskykh roslyn. Kharkiv, 2018. P. 43–44.

Borodina N., Koshovyi O., Kovalyov V. The chromatography-mass spectrometry study of Salix rosmarinifolia L // Azerbaidzhanskii farmatsevticheskii i Farmakoterapevticheskii zhurnal. 2016. Vol. XVI, Issue 2. P. 15–20.

Borodina N. V., Kovalov V. M. Mineralnyi sklad deiakykh vydiv rodyny Salicaceae // Zbirnyk naukovykh prats spivrobitnykiv NMAPO im. P. L. Shupyka. 2018. Issue 29. P. 180–187.

Chernogorod L. B., Vinogradov B. A. Efirnye masla nekotorykh vidov roda Achillea L., soderzhashhie fragranol // Rastitelnye resursy. 2006. Vol. 42, Issue 2. P. 61–68.

Levytskyi A. P., Khodakov Y. V., Raitseva E. S. Ekstraktsyia polyfenolov yz lystev vynohrada // Kharchova nauka i tekhnolohiia. 2012. Vol. 20, Issue 3. P. 36–37.

Sposib identyfikatsii polifenoliv v roslynnykh ekstraktakh: Pat. No. 80597 UA. MPK (2013.01) G01N 33/00 / Khodakov I. V., Levytskyi A. P., Makarenko O. A. No. u 201212473; declareted: 31.10.2012; published: 10.06.2013. Bul. No. 11.

Frukty, ovochi ta produkty yikh pererobliannia. Metody vyznachennia polifenoliv. DSTU 4373:2005 (Natsionalnyi standart Ukrainy). Kyiv: Derzhspozhyvstandart Ukrainy, 2006. 6 p.

Vertikova E. K., Khodakov I. V., Levitskii A. P. Metod opredeleniia khlorogenovoi kisloty // Vіsnik stomatologіi. Spets. vipusk. 2010. Vol. 73, Issue 5. P. 2–5.

Kilkisne vyznachennia flavonoidiv u losionakh dlia likuvannia infektsiinykh zakhvoriuvan shkiry / Rekhletska O. V., Kalyniuk T. H., Hudz N. I., Fedushchak N. K. // Medychna khimiia. 2007. Vol. 9, Issue 2. P. 87–905.

Khodakov I. V. Sposob identifikatsii polifenolov v rastitelnykh ekstraktakh pri pomoshhi VEZHKH. Opredelenie sostava izoflavonov soi // Metody i obekty khimicheskogo analiza. 2013. Vol. 8, Issue 3. P. 132–142.

Khodakov I. V. Sposob identifikatsii polifenolov v rastitelnykh ekstraktakh // Vіsnik stomatologіi. Spets. vip. 2012. Vol. 80, Issue 7. P. 42.

Sortovye osobennosti soi ukrainskoi selektsii po soderzhaniiu polifenolov v listiakh / Khodakov I. V., Makarenko O. A., Levitskii A. P., Sichkar V. I. // Fiziologiia rastenii i genetika. 2014. Vol. 46, Issue 1. P. 27–36.







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