Comparative study of phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.225767

Keywords:

Betonica peraucta Klokov, Betonica brachydonta Klokov, herb, phenolic compounds, HPLC, spectrophotometry

Abstract

Species of Betonica L. genus are widespread in Ukraine and contain different groups of biologically active substances: hydroxycinnamic acids, flavonoids, tannins, iridoids, terpenoids, steroids, essential oil, organic acids, vitamin K, nitrogen-containing compounds, phenylethanoid glycosides. Species of Betonica L. genus show a wide range of pharmacological activity (anti-inflammatory, antioxidant, choleretic, diuretic, sedative, antitumor, antihypertensive, etc.) and phenolic compounds are one of the most important and promising groups of biologically active substances of these plants.

The aim. The aim of the work was to conduct a comparative study of the phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine.

Materials and methods. The object of the study was the herb of Betonica peraucta and Betonica brachydonta harvested in the phase of mass flowering of the plant in Ivano-Frankivsk region. The study of phenolic compounds was carried out by paper chromatography, HPLC and spectrophotometry.

Results. 7 components of tannins, 4 flavonoids, 5 hydroxycinnamic acids, 2 coumarins were identified and quantified by HPLC in the studied raw material. The quantitative content of the main groups of phenolic compounds in the herb of Betonica peraucta and Betonica brachydonta was determined by the method of absorption spectrophotometry: polyphenols – 5.96 % and 4.82 %, tannins – 1.62 % and 0.68 %, flavonoids – 2.07 % and 1.13 %, hydroxycinnamic acids – 7.01 % and 3.58 %, respectively.

Conclusions. As a result of the conducted studies it was found that the content of phenolic compounds in the herb of Betonica peraucta is significantly higher than in the herb of Betonica brachydonta. Therefore, this species is promising for further research and creation of new drugs

Author Biographies

Iryna Sas, Ivano-Frankivsk National Medical University

PhD, Assistant

Department of Pharmacy

Andriy Grytsyk, Ivano-Frankivsk National Medical University

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmacy

Taras Koliadzhyn , Ivano-Frankivsk National Medical University

PhD, Assistant

Department of Pharmacy

Oleh Koshovyi , National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of the Department

Department of Pharmacognosy

References

  1. Dimitrova-Dyulgerova, I., Merdzhanov, P., Todorov, K., Seymenska, D., Stoyanov, P., Mladenov, R., Stoyanova, A. (2015). Essential Oils Composition of Betonica officinalis L. and Stachys sylvatica L. (Lamiaceae) from Bulgaria. Proceedings of the Bulgarian Academy of Sciences, 68 (8), 991–998.
  2. Bączek, K., Kosakowska, O., Przybył, J. L., Węglarz, Z. (2016). Accumulation of phenolic compounds in the purple betony herb (Stachys officinalis L.) originated from cultivation. Herba Polonica, 62 (2), 7–16. doi: http://doi.org/10.1515/hepo-2016-0007
  3. Grytsyk, L., Grytsyk A., Sas I., Legin N., Kolyadjin T. (2016). Study of aminoacid composition of Betonica L., Sanicula L., and Astrantia L. Genera Species. The Pharma Innovation Journal, 5 (7), 46–48.
  4. Tzanova, M., Grozeva, N., Gerdzhikova, M., Argirova, M., Pavlov, D., Terzieva, S. (2018). Flavonoid content and antioxidant activity of Betonica bulgarica Degen et Neič. Bulgarian Chemical Communications, 50 (C), 90–97.
  5. Paun, G., Neagu, E., Moroeanu, V., Ungureanu, O., Cretu, R., Ionescu, E. et. al. (2017). Phytochemical analysis and in vitro biological activity of Betonica officinalis and Salvia officinalis extracts. Romanian Biotechnological Letters, 22 (4), 12751–12761.
  6. Paun, G., Neagu, E., Albu, C., Moroeanu, V., Radu, G.-L. (2016). Antioxidant activity and inhibitory effect of polyphenolic-rich extract from Betonica officinalis and Impatiens noli-tangere herbs on key enzyme linked to type 2 diabetes. Journal of the Taiwan Institute of Chemical Engineers, 60, 1–7. doi: http://doi.org/10.1016/j.jtice.2015.10.005
  7. Paun, G., Neagu, E., Moroeanu, V., Albu, C., Ursu, T.-M., Zanfirescu, A. et. al. (2018). Anti-inflammatory and antioxidant activities of the Impatiens noli-tangere and Stachys officinalis polyphenolic-rich extracts. Revista Brasileira de Farmacognosia, 28 (1), 57–64. doi: http://doi.org/10.1016/j.bjp.2017.10.008
  8. Uritu, C. M., Mihai, C. T., Stanciu, G.-D., Dodi, G., Alexa-Stratulat, T., Luca, A. et. al. (2018). Medicinal Plants of the Family Lamiaceae in Pain Therapy: A Review. Pain Research and Management, 2018, 1–44. doi: http://doi.org/10.1155/2018/7801543
  9. Hrytsyk, A., Sas, I. (2017). Hostra toksychnist ta antyeksudatyvna aktyvnist ekstraktiv travy bukvytsi perebilshenoi (Betonica peraucta Klok.) ta bukvytsi korotkozuboi (Betonica brachydonta Klok.). Farmakom, 4, 29–33.
  10. Sas, I. (2019). Doslidzhennia sedatyvnoi aktyvnosti ekstraktiv vydiv rodu Bukvytsia – Betonica (L.). Farmakom, 4, 23–27.
  11. Dobrochaeva, D. N., Kotov, M. I., Prokudin, Yu. N., Barbarich, A. I. (1999). Opredelitel vysshyh rasteniy Ukrainy. Kyiv: Fitosotsyotsentr, 548.
  12. Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  13. Starchenko, G., Hrytsyk, A., Raal, A., Koshovyi, O. (2020). Phytochemical Profile and Pharmacological Activities of Water and Hydroethanolic Dry Extracts of Calluna vulgaris (L.) Hull. Herb. Plants, 9 (6), 751. doi: http://doi.org/10.3390/plants9060751
  14. Krivoruchko, E., Markin, A., Samoilova, V., Ilina, T., Koshovyi, O. (2018). Research in the chemical composition of the bark of sorbus aucuparia. Ceska a Slovenska Farmacie, 67 (3), 113–115.
  15. Koshovyi, O., Raal, A., Kovaleva, A., Myha, M., Ilina, T., Borodina, N., Komissarenko, A. (2020). The phytochemical and chemotaxonomic study of Salvia spp. growing in Ukraine. Journal of Applied Biology & Biotechnology, 8 (3), 29–36. doi: http://doi.org/10.7324/JABB.2020.80306
  16. Derzhavna Farmakopeia Ukrainy. Vol. 3 (2014). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 732.
  17. Ilina, T., Kashpur, N., Granica, S., Bazylko, A., Shinkovenko, I., Kovalyova, A. et. al. (2019). Phytochemical Profiles and In Vitro Immunomodulatory Activity of Ethanolic Extracts from Galium aparine L. Plants, 8 (12), 541. doi: http://doi.org/10.3390/plants8120541
  18. Shestakova, T., Belonohova, V., Petrychenko, V. (2016). Spektrofotometricheskiy metod opredeleniya soderzhaniya flavonoidov v trave Veronica chamaedrys (Scrophulariaceae). Meditsynskiy almanah, 1 (41), 127–130.
  19. Štefan, M. B., Vuković Rodríguez, J., Blažeković, B., Kindl, M., & Vladimir-Knežević, S. (2013). Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species. Food Analytical Methods, 7 (2), 326–336. doi: http://doi.org/10.1007/s12161-013-9630-8
  20. Thiese, M., Arnold, Z., Walker, S. (2015). The misuse and abuse of statistics in biomedical research. Biochemia Medica, 25 (1), 5–11. doi: http://doi.org/10.11613/BM.2015.001
  21. Hajdari, A., Mustafa, B., Franz, Ch., Novak, J. (2010). Total flavonoids, total phenolics and antioxidant activity of Betonica officinalis L. from Kosovo. Acta Horticulturae, 860, 75–80. doi: http://doi.org/10.17660/actahortic.2010.860.8
  22. Imbrea, I., Butnariu, M., Nicolin, A., Imbrea, F., Prodan, M. (2011). Valorising the Species Stachys officinalis (L.) Trevis. from South-Western Romania. Research Journal of Agricultural Science, 43 (2), 198–203.

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Published

2021-02-27

How to Cite

Sas, I., Grytsyk, A., Koliadzhyn , T. ., & Koshovyi , O. . (2021). Comparative study of phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine. ScienceRise: Pharmaceutical Science, (1 (29), 66–75. https://doi.org/10.15587/2519-4852.2021.225767

Issue

No. 1 (29) (2021)

Section

Pharmaceutical Science

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Copyright (c) 2021 Ирина Анатольевна Сас , Андрей Романович Грицик , Тарас Иванович Коляджин , Олег Миколайович Кошовий

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