A novel comparative chromatographic research of secoiridoid glycosides in two species of centaury herb

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.235774

Keywords:

Centaurium erythraea, Cеntaurium pulchellum, secoiridoid glycosides, swertiamarin, HPLC, validation

Abstract

The aim: A novel comparative analysis of the secoiridoid glycosides composition in Centaurium erythraea Rafn. and Cеntaurium pulchellum (Sw.) Druce has being described. Swertiamarin has been chosen as an active marker and its comparative quantitative estimation in two species of the raw material has been carried out by HPLC method.

Materials and methods: The quantitative HPLC analysis of swertiamarin was conducted using a chromatographic column ACE 5 C18. Methanol and 0,5 % acetic acid aqueous solution were used as mobile phases; the chromatographic procedure was carried out in a gradient mode.

Results: The content of swertiamarin in the C. pulchellum herb ranges from 2.51 to 3.07 mg/100 mg. In that time swertiamarin content in C. erythraea herb varies and depends from 3.83 to 8.94 mg/100 mg. The content of swertiamarin in C. pulchellum herb is much lower than in C. erythraea herb. Taking into account this fact the possibility of using of C. pulchellum herb for medicine instead of C. erythraea herb must be proven additionally by biological experiments. Also it could be preliminary concluded that the most appropriate climate for swertiamarin accumulation in C. erythraea herb is located in Central and Eastern parts of Ukraine.

Conclusions: It was discovered that the main representative of secoiridoid glycosides in C. erythraea is swertiamarin when in C. pulchellum Druce that are represented by both sweroside and swertiamarin. A more perspective raw material – C. erythraea herb, according to the content of swertiamarin, was chosen

Author Biographies

Svitlana Gubar, National University of Pharmacy

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Anna Materiienko, National University of Pharmacy

PhD, Assistant

Department of Quality, Standardization and Certification of Drugs

Liudas Ivanauskas, Lithuanian University of Health Sciences

Doctor of Biomedical Sciences, Professor, Head of Department

Department of Analytical and Toxicological Chemistry

Volodymyr Mishchenko, National University of Pharmacy

PhD, Associate Professor

Department of Quality, Standardization and Certification of Drugs

Olha Vasylieva, National University of Pharmacy

PhD, Associate Professor

Department of Quality, Standardization and Certification of Drugs

Victoriya Georgiyants, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Chemistry

References

  1. Siler, B., Misic, D. (2016). Chapter 11 - Biologically Active Compounds from the Genus Centaurium s.l. (Gentianaceae): Current Knowledge and Future Prospects in Medicine Studies in Natural Products Chemistry. Elsevier, 49, 363–397. doi: http://doi.org/10.1016/b978-0-444-63601-0.00011-9
  2. Petropoulos, S. A., Karkanis, A., Martins, N., Ferreira, I. C. F. R. (2018). Halophytic herbs of the Mediterranean basin: An alternative approach to health. Food and Chemical Toxicology, 114, 155–169. doi: http://doi.org/10.1016/j.fct.2018.02.031
  3. Hamza, N., Berke, B., Cheze, C., Marais, S., Lorrain, S., Abdouelfath, A. et. al. (2015). Effect of Centaurium erythraea Rafn, Artemisia herba-alba Asso and Trigonella foenum-graecum L. on liver fat accumulation in C57BL/6J mice with high-fat diet-induced type 2 diabetes. Journal of Ethnopharmacology, 171, 4–11. doi: http://doi.org/10.1016/j.jep.2015.05.027
  4. Stefkov, G., Miova, B., Dinevska-Kjovkarovska, S., Stanoeva, J. P., Stefova, M., Petrusevska, G., Kulevanova, S. (2014). Chemical characterization of Centaurium erythrea L. and its effects on carbohydrate and lipid metabolism in experimental diabetes. Journal of Ethnopharmacology, 152(1), 71–77. doi: http://doi.org/10.1016/j.jep.2013.11.047
  5. Tuluce, Y., Ozkol, H., Koyuncu, I., Ine, H. (2011). Gastroprotective effect of small centaury (Centaurium erythraea L) on aspirin-induced gastric damage in rats. Toxicology and Industrial Health, 27 (8), 760–768. doi: http://doi.org/10.1177/0748233710397421
  6. Patel, M. B., Mishra, S. H. (2011). Hypoglycemic activity of C-glycosyl flavonoid fromEnicostemma hyssopifolium. Pharmaceutical Biology, 49 (4), 383–391. doi: http://doi.org/10.3109/13880209.2010.517759
  7. El Menyiy, N., Guaouguaou, F.-E., El Baaboua, A., El Omari, N., Taha, D., Salhi, N. et. al. (2021). Phytochemical properties, biological activities and medicinal use of Centaurium erythraea Rafn. Journal of Ethnopharmacology, 276, 114171. doi: http://doi.org/10.1016/j.jep.2021.114171
  8. Aberham, A., Pieri, V., Croom, E. M., Ellmerer, E., Stuppner, H. (2011). Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC–MS and RP-HPLC. Journal of Pharmaceutical and Biomedical Analysis, 54 (3), 517–525. doi: http://doi.org/10.1016/j.jpba.2010.09.030
  9. Boroduske, A., Nakurte, I., Tomsone, S., Lazdane, M., Boroduskis, M., Rostoks, N. (2016). In vitro culture type and elicitation affects secoiridoid and xanthone LC–ESI–TOF MS profile and production in Centaurium erythraea. Plant Cell, Tissue and Organ Culture (PCTOC), 126 (3), 567–571. doi: http://doi.org/10.1007/s11240-016-1016-3
  10. Shikov, A. N., Pozharitskaya, O. N., Makarov, V. G., Wagner, H., Verpoorte, R., Heinrich, M. (2014). Medicinal Plants of the Russian Pharmacopoeia; their history and applications. Journal of Ethnopharmacology, 154 (3), 481–536. doi: http://doi.org/10.1016/j.jep.2014.04.007
  11. European Pharmacopoeia (2017). Strasbourg: European Directorate for the Quality of Medicines & Health Care.
  12. Sharma, M. K., Bachwani, M. (2013). Significance of Plant Bitters In The Field of Pharmacognosy. Asian Journal of Pharmaceutical Technology & Innovation, 1 (3), 1–14.
  13. Jaishree, V., Badami, S. (2010). Antioxidant and hepatoprotective effect of swertiamarin from Enicostemma axillare against d-galactosamine induced acute liver damage in rats. Journal of Ethnopharmacology, 130 (1), 103–106. doi: http://doi.org/10.1016/j.jep.2010.04.019
  14. Proskurova, Ya., Gubar, S., Kotova, E., Kotov, A., Datkhayev, U. (2017). Development of the method for Centaury herb identification by thin layer chromatography for the State Pharmacopoeia of Ukraine monograph. Bulletin of National Academy of sciences of the Republic of Kazakhstan, 2 (5), 5–12.
  15. Proskurova, Y. A., Gubar, S. N., Kotova, E. E., Kotov, A. G. (2016). A comparative analysis of european and german pharmacopoeias requirements to the “bitterness value” test. ScienceRise, 4 (4 (21)), 65–68. doi: http://doi.org/10.15587/2313-8416.2016.67687
  16. ICH harmonized tripartite guideline Q2(R1). Validation of analytical procedures: text and methodology Q2(R1) (2005). Proceedings of the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use. Geneva. Available at: https://database.ich.org/sites/default/files/Q2 %28R1 %29 %20Guideline.pdf
  17. State Pharmacopoeia of Ukraine. Vol. 3 (2014). Kharkiv: State enterprise “Ukrainian scientific pharmacopoeial center of medicines quality”.
  18. Xiong, K., Gao, T., Zhang, T., Wang, Z., Han, H. (2017). Simultaneous determination of gentiopicroside and its two active metabolites in rat plasma by LC–MS/MS and its application in pharmacokinetic studies. Journal of Chromatography B, 1065-1066, 1–7. doi: http://doi.org/10.1016/j.jchromb.2017.09.017
  19. Glatz, Z., Pospísilová, J., Musil, P. (2000). Determination of gentiopicroside in extracts of centaurium erythreae and gentiana lutea by micellar electrokinetic capillary chromatography. Journal of Liquid Chromatography & Related Technologies, 23 (12), 1831–1839. doi: http://doi.org/10.1081/jlc-100100454
  20. Xu, Y., Li, Y., Maffucci, K., Huang, L., Zeng, R. (2017). Analytical Methods of Phytochemicals from the Genus Gentiana. Molecules, 22 (12), 2080. doi: http://doi.org/10.3390/molecules22122080
  21. Alam, P., Ali, M., Singh, R., Shakeel, F. (2009). Estimation of swertiamarin in Enicostemma littorale and marketed formulations using HPLC-UV Method. Journal of Bioanalysis and Biomedicine, 1 (1), 22–27.
  22. Kshirsagar, P. R., Pai, S. R., Nimbalkar, M. S., Gaikwad, N. B. (2015). RP-HPLC analysis of seco-iridoid glycoside swertiamarin from differentSwertiaspecies. Natural Product Research, 30 (7), 865–868. doi: http://doi.org/10.1080/14786419.2015.1071366
  23. Gubar, S. M., Materiienko, A. S., Smielova, N. M., Budanova, L. G., Georgiyants, V. A. (2020). Development of a New Approach for Standardization of the Herb Centaurium erythraea Rafn. by High Performance Liquid Chromatography. Turkish Journal of Pharmaceutical Sciences, 17 (6), 593–598. doi: http://doi.org/10.4274/tjps.galenos.2019.71542
  24. Pataczek, L., Cheilari, A., Zikeli, S., Sturm, S., Stuppner, H., Gruber, S. (2017). Centaurium erythraea Cultivation Method for Optimal Yield and Product Quality. Journal of Herbs, Spices & Medicinal Plants, 23 (3), 193–215. doi: http://doi.org/10.1080/10496475.2017.1295414

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Published

2021-06-30

How to Cite

Gubar, S., Materiienko, A., Ivanauskas, L., Mishchenko, V., Vasylieva, O., & Georgiyants, V. (2021). A novel comparative chromatographic research of secoiridoid glycosides in two species of centaury herb. ScienceRise: Pharmaceutical Science, (3(31), 28–33. https://doi.org/10.15587/2519-4852.2021.235774

Issue

No. 3(31) (2021)

Section

Pharmaceutical Science

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Copyright (c) 2021 Svitlana Gubar, Anna Materiienko, Liudas Ivanauskas, Volodymyr Mishchenko, Olha Vasylieva, Victoriya Georgiyants

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