Research in components of essential oils from flowers and leaves of the genus Alchemilla L. species

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.259059

Keywords:

Alchemilla, Alchemilla. flabellata Bus., Alchemilla phegophila Juz., Alchemilla subrenata Bus., flowers, leaves, essential oil, chromato-mass spectrometry

Abstract

The genus Alchemilla L. has about 1000 species in the world flora, of which 35 grow in Ukraine. The most common in Ukraine are A. flabellata Bus., A. phegophila Juz. and A. subrenata Bus. Despite the widespread distribution of species of the genus Alchemilla L. and the publication of a monograph on the herb of the collective species - Herba Alchemillae (Alchemiilla vulgaris L. sensu latiore) in the State Pharmacopoeia of Ukraine, the chemical composition, and pharmacological properties of species of the genus Alchemilla L. are insufficiently studied, therefore, it is advisable to study the chemical composition of the most common species of flora of Ukraine for their introduction into medical and pharmaceutical practice.

The aim of the work was to study the component composition and quantitative content of essential oils of flowers and leaves of some species of the genus Alchemilla L.

Materials and methods. Flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus. harvested in the Ivano-Frankivsk region in 2020 - 2021.

The component composition and quantitative content of essential oils were determined by chromato-mass spectrometric method. The compounds were identified by comparing the obtained mass spectra of the chromatographic peak with the mass spectra of the reference compounds and based on comparison with the spectra of the database. Quantitative determination of the content of substances in the raw material was performed in comparison with a standard sample of menthol.

Results. The essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus were obtained. It was found that the highest content of essential oils was characterized by Alchemilla. flabellata Bus. flowers (16884.6 mg / kg), and the least essential oil was contained in the leaves of Alchemilla Phegophila Juz. (4895.5 mg / kg). As a result of studying the component composition of essential oils of flowers and leaves of Alchemilla flabellata Bus., Alchemilla Phegophila Juz. and Alchemilla subrenata Bus. 48, 51 and 47 compounds were identified, of which 44, 48 and 43 were identified, respectively. 31 components of essential oil were common in the studied samples of raw materials.

Conclusions. Due to the component composition of the essential oil of flowers and leaves of Alchemilla. flabellata Bus., Alchemilla phegophila Juz. and Alchemilla subrenata Bus., as well as considering the known pharmacological activity of its components, it is advisable to conduct further pharmacological studies of raw materials of the genus Alchemilla L. to study their antibacterial, anti-inflammatory and antitumor properties

Author Biographies

Nataliia Dubel, Ivano-Frankivsk National Medical University

PhD, Assistant

Departments of Pharmaceutical Management, Drug Technology and Pharmacognosy

Andriy Grytsyk, Ivano-Frankivsk National Medical University

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Departments of Pharmaceutical Management, Drug Technology and Pharmacognosy

Lyubov Grytsyk, Ivano-Frankivsk National Medical University

PhD, Associate Professor

Department of Chemistry, Pharmaceutical Analysis and Postgraduate Education

Oleh Koshovyi, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy

Alla Kovaleva, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy

References

  1. Gehrke, B., Bräuchler, C., Romoleroux, K., Lundberg, M., Heubl, G., Eriksson, T. (2008). Molecular phylogenetics of Alchemilla, Aphanes and Lachemilla (Rosaceae) inferred from plastid and nuclear intron and spacer DNA sequences, with comments on generic classification. Molecular Phylogenetics and Evolution, 47 (3), 1030–1044. doi: http://doi.org/10.1016/j.ympev.2008.03.004
  2. Boruz, V. (2010) Taxonomy, chorology, ecology and coenology of the Alchemilla flabellate species. Research Journal of Agricultural Sciences, 42 (2), 216–223.
  3. Bradshaw, M. E. (2009). The decline of Lady’s-mantles (Alchemilla vulgaris L. agg.) and other hay-meadow species in Northern England since the 1950s. Watsonia, 27, 315–321.
  4. Gavrilova, A., Vitkova, A. (2010). Distribution and Ecology of Alchemilla Species in Osogovo Mt. and West Balkan Mt. In Bulgaria. Hacquetia, 9 (1), 77–90. doi: http://doi.org/10.2478/v10028-010-0004-0
  5. Kurtto, A., Frohner, S., Lampinen, R. et. al. (2007). Rosaceae (Alchemilla and Aphanes). Helsinki: The Committee for Mapping the Flora of Europe & Societas Biologica Fermica Vanamo, 200.
  6. Boruz, V. (2011). The morpho-anatomy of Alchemilla flabellatа species. Research Journal of Agricultural Sciences, 16, 59–65.
  7. European Pharmacopoeia (2005). Council of Europe, Strasbourg, 935.
  8. Falcheroa, L., Coppaa, M., Espostib, S., Tava, A. (2008). Essential Oil Composition of Alchemilla alpina L. em. Buser from Western Alpine Pastures. Journal of Essential Oil Research, 20 (6), 542–545. doi: http://doi.org/10.1080/10412905.2008.9700084
  9. Grytsyk, A. R., Grytsyk, L. M., Tuchak, N. I. (2011). Perspektyvy vykorystannya roslyn rodu Pryvoroten u medytsyni ta farmatsiyi. Lviv: DP MVS Ukrayiny « Lviv – Inform – Resursy», 64.
  10. Derzhavna Farmakopeia Ukrainy. Dopovnennya 5. (2021). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 424.
  11. Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  12. Bicchi, C., Brunelli, C., Cordero, C., Rubiolo, P. et. al. (2004). Methods of the chromate-mass-spectrometric research. Journal of Chromatography A, 1-2, 195–207.
  13. Zenkevich, I. G., Kochetova, M. V., Larionov, O. G., Revina, A. A. (2005). Indeksy uderzhivaniya kak naiboleye vosproizvodimyye khromatograficheskiye parametry dlya kharakteristiki fenolnykh soyedineniy v obrashchenno-fazovoy vysokoeffektivnoy zhidkostnoy khromatografii. Zhurnal analіtichnoi khіmіi, 60 (7), 734–746.
  14. Tsurkan, O. O., Kovalchuk, T. V., Herhel, O. V. (2012). Khromato-mas-spektrometrychne doslidzhennya letkykh komponentiv nadzemnoyi chastyny shovkovytsi. Farmakolohiya ta likarska toksykolohiya, 1 (26), 54–59.
  15. Koshovyi, O., Raal, A., Kireyev, I., Tryshchuk, N., Ilina, T., Romanenko, Y. et. al. (2021). Phytochemical and Psychotropic Research of Motherwort (Leonurus cardiaca L.) Modified Dry Extracts. Plants, 10 (2), 230. doi: http://doi.org/10.3390/plants10020230
  16. Borodina, N. V., Kovalyov, V. V., Koshovyi, O. M., Akhmedov, E. Y. (2016). The chromatography-mass spectrometry study of Salix rosmarinifolia L. Azerbaijan Pharmaceutical and Pharmacotherapy Journal, 16 (2), 15–20.
  17. Kadri, A., Zarai, Z., Beacute, A., Gharsallah, N., Damak, M., Gdoura, R. (2011). Chemical constituents and antioxidant activity of the essential oil from aerial parts of Artemisia herba-alba grown in Tunisian semi-arid region. African Journal of Biotechnology, 10 (15), 2923–2929. doi: http://doi.org/10.5897/ajb10.2491
  18. Starchenko, G., Hrytsyk, A., Raal, A., Koshovyi, O. (2020). Phytochemical profile and pharmacological activities of water and hydroethanolic dry extracts of Calluna vulgaris (L.) Hull. herb. Plants, 9, 751. doi: http://doi.org/10.3390/plants9060751
  19. Jugreet, B. S., Mahomoodally, M. F. (2020). Pharmacological Properties of Essential Oil Constituents and their Mechanisms of Action. Plant-Derived Bioactives. Singapore: Springer, 387–415. doi: http://doi.org/10.1007/978-981-15-2361-8_18
  20. Ilina, T., Skowrońska, W., Kashpur, N., Granica, S., Bazylko, A., Kovalyova A. et. al. (2020). Immunomodulatory Activity and Phytochemical Profile of Infusions from Cleavers Herb. Molecules, 25, 3721. doi: http://doi.org/10.3390/molecules25163721
  21. Sipovo Tala, V. R., Candida da Silva, V. C., Rodrigues, C. M., Nkengfack, A., dos Santos, L. C., Vilegas, W. (2013). Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis – Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Cromatography. Electrospray Ionization Mass Spectrometry, 18, 2803–2820. doi: http://doi.org/10.3390/molecules18032803
  22. Aljaafari, M. N., AlAli, A. O., Baqais, L., Alqubaisy, M., AlAli, M., Molouki, A. et. al. (2021). An Overview of the Potential Therapeutic Applications of Essential Oils. Molecules, 26 (3), 628. doi: http://doi.org/10.3390/molecules26030628
  23. Elshafie, H. S., Camele, I. (2017). An Overview of the Biological Effects of Some Mediterranean Essential Oils on Human Health. BioMed Research International, 2017, 1–14. doi: http://doi.org/10.1155/2017/9268468
  24. Sydora, N., Konovalova, O., Zuikina, S., Semchenko, K., Rudnyk, A., Hurtovenko, I. (2021). Comparative study the essential oil composition of flowers and leaves of Crataegus monogyna L. ScienceRise: Pharmaceutical Science, 6 (34), 20–26. doi: http://doi.org/10.15587/2519-4852.2021.249276
  25. Myha, M., Koshovyi, O., Karpun, Y., Kovaleva, A., Mala, O., Parchenko, V. et. al. (2021). Chromato-mass-spectrometric research in Salvia grandiflora L., Salvia pratensis L. and Salvia verticillata L. aboveground organs. ScienceRise: Pharmaceutical Science, 5 (33), 32–40. doi: http://doi.org/10.15587/2519-4852.2021.242761
  26. Giordani, R., Hadef, Y., Kaloustian, J. (2008). Compositions and antifungal activities of essential oils of some Algerian aromatic plants. Fitoterapia, 79 (3), 199–203. doi: http://doi.org/10.1016/j.fitote.2007.11.004
  27. Marques, A., Teixeira, B., Nunes, M. L. (2016). Bay Laurel (Laurus nobilis) Oils. Essential Oils in Food Preservation, Flavor and Safety, 239–246. doi: http://doi.org/10.1016/b978-0-12-416641-7.00026-2
  28. Caputo, L., Nazzaro, F., Souza, L., Aliberti, L., De Martino, L., Fratianni, F. et. al. (2017). Laurus nobilis: Composition of Essential Oil and Its Biological Activities. Molecules, 22 (6), 930. doi: http://doi.org/10.3390/molecules22060930

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Published

2022-06-30

How to Cite

Dubel, N., Grytsyk, A., Grytsyk, L., Koshovyi, O., & Kovaleva, A. (2022). Research in components of essential oils from flowers and leaves of the genus Alchemilla L. species. ScienceRise: Pharmaceutical Science, (3(37), 34–39. https://doi.org/10.15587/2519-4852.2022.259059

Issue

No. 3(37) (2022)

Section

Pharmaceutical Science

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Copyright (c) 2022 Nataliia Dubel, Andriy Grytsyk, Lyubov Grytsyk, Oleh Koshovyi, Alla Kovaleva

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