Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.266400

Keywords:

Tanacetum parthenium, extract, phenolic profile, hydroxycinnamic acids, antiradical activity, anti-inflammatory activity

Abstract

The aim – to study the phenolic complex of a thick hydroalcoholic extract of the feverfew (Tanacetum parthenium (L.) herb (FTHAE), its antiradical activity and anti-inflammatory properties in a model of carrageenan and histamine oedema.

Materials and methods. The studied extract was obtained from the Tanacetum parthenium herb, collected in Sumy and Poltava regions of Ukraine during the period of mass flowering (June-August): degree of grinding of raw materials 2.0-3.0 mm, extraction temperature - 25 °C, extractant – 70 % ethanol, raw material/extractant ratio – 1:12, infusion time – 12 hours, multiplicity of extractions – 3. HPLC and spectrophotometric methods were used to determine the composition and amount of phenolic compounds of FTHAE. HPLC analysis was performed using a “Waters e2695 Alliance system” (Waters, Milford, MA, USA) with a photodiode array detector “Waters 2998” according to the HPLC–PDA method for phenolic compounds. The scavenging of ABTSA radical cation evaluated the radical scavenging activity. In addition, the anti-inflammatory properties of FTHAE were studied on carrageenan and histamine paw oedema in rats. Anti-inflammatory activity (AIA) was evaluated as the ability to reduce oedema compared to the control pathology. FTHAE was used at a dose of 50 mg/kg.

The results. The content of the sum of hydroxycinnamic acids in the obtained extract was determined by spectrophotometry, which was 13.92±0.02 % and the content of the sum of flavonoids – 5.16±0.03 %. The content of 12 compounds with a total amount of 72432.09µg/g was identified and determined by HPLC. The dominant compounds were hydroxycinnamic acids, namely 3,4-dicaffeoylquinic, 4,5-dicaffeoylquinic and сhlorogenic acids. The antiradical activity of the extract was 620.19±4.53µmol/g. On the model of carrageenan oedema, the maximum effect of oedema suppression was 71.0-73.2 %. In the model of histamine oedema, the anti-inflammatory effect of the extract was 57.8; 51.8; and 49.1 % for 30 minutes, 1 and 1.5 hours of oedema, respectively. The severity of the anti-inflammatory activity of the extract during the first hour is not inferior to the diclofenac sodium, quercetin and loratadine.

Conclusions. Due to the HPLC method, 12 compounds were determined to cause antiradical activity, among which chlorogenic acid and rutin were identified.

The studied extract has a pronounced anti-inflammatory effect, which is due to the antiradical properties of the extract and its inhibitory effect on inflammatory mediators

Author Biographies

Oksana Mishchenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Clinical Pharmacology

Institute of Advanced Training of Pharmacy Specialists

Inna Kyrychenko, National University of Pharmacy

Department of Clinical Pharmacology

Institute of Advanced Training of Pharmacy Specialists

Tetiana Gontova, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy

Kateryna Kalko, National University of Pharmacy

PhD, Associate Professor

Department of Clinical Pharmacology

Institute of Advanced Training of Pharmacy Specialists

Karyna Hordiei, LLC Intego Group

PhD, Clinical Data Manager

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Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract

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2022-10-31

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Mishchenko, O., Kyrychenko, I., Gontova, T., Kalko, K., & Hordiei, K. (2022). Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract. ScienceRise: Pharmaceutical Science, (5 (39), 91–99. https://doi.org/10.15587/2519-4852.2022.266400

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No. 5 (39) (2022)

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Pharmaceutical Science

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Copyright (c) 2022 Oksana Mishchenko, Inna Kyrychenko, Tetiana Gontova, Kateryna Kalko, Karyna Hordiei

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