Antibiofilm activity of azithromycin against Staphylococcus epidermidis
DOI:
https://doi.org/10.15587/2519-8025.2018.128653Keywords:
biofilms, Staphylococcus, antibiotics, mechanisms of effect, adhesion, hydrophobic behavior, red Kongo, РІАAbstract
The aim of the study was to establish specific activity of azithromycin against the biofilm, formed by S. epidermidis and several aspects of the mechanism of the effect of this preparation.
The ability of Staphylococcus to adhesion on abiotic surfaces (polysterene plane-tables) was determined by Christensen methodology (fixation by 96 % ethanol, coloration by 0.1 % solution of gentian violet). The antibiofilm activity of azithromycin was investigated by the method of sorption of gentian violet on biofilm structures with further desorption in 96 % ethyl alcohol. The hydrophobic behavior of a strain was investigated using BATH-test in the two-phased system with ethylacetate. For determining the ability of epidermal Staphylococcus to producing polysaccharide intercellular adhesin (PIA) there was used Luria-Bertani (LB) agarized medium with 0,08 % red Kongo.
The study of the antibiofilm activity of azithromicin demonstrated that the antibiotic disturbs fixation to the abiotic surface and film formation of S. epidermidis 2265 in concentration 5.0 MIC, but doesn’t have the inhibiting effect of the formed 1-day film. It was established, that a clinical strain of epidermal Staphylococcus S. epidermidis 2265 has no ability to production of polysaccharide intercellular adhesion (РІА).
The studies demonstrated that the effect of azithromycin is directed on the primary stages of biofilm formation. It was established, that the mechanism of the effect of the antibiotic on biofilm bacteria is not conditioned by a change of the hydrophobic behavior of the bacterial surface and PIA production
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Copyright (c) 2018 Nataliia Hrynchuk, Nina Vrynchanu, Larysa Stepura
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