Level of Cyclin D1 protein in peripheral blood lymphocytes of Chornobyl clean-up workers in remote period after radiation exposure

Authors

  • Liliia Zvarych State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050, Ukraine https://orcid.org/0000-0003-1805-1319
  • Nataliya Golyarnik State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050, Ukraine https://orcid.org/0000-0002-8760-5859
  • Iryna Ilienko State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050, Ukraine https://orcid.org/0000-0003-4405-0328

DOI:

https://doi.org/10.15587/2519-8025.2019.165703

Keywords:

Cyclin D1, cellular cycle, genome instability, lymphocytes, ionizing irradiation, CAES accident

Abstract

Aim: to assess changes of products of Cyclin D1 protein in lymphocytes of peripheral blood of Chernobyl clean-up workers as remote results of the action of ionizing irradiation.   

Methods: there were examined 120 Chernobyl clean-up workers in the remote period after radiation exposure and 45 persons of the control group. For assessing mitogen-induced levels of Cyclin D1, the micro-method of cultivating erythrocytes of whole blood was used. The quantitative assessment of the spontaneous and mitogen-induced levels of Cyclin D1 in lymphocytes of peripheral blood (PB) was realized using the reagents FITC Mouse Anti-Human Cyclin D1 Antibody Set (BD, USA) by the method of flow cytometry.

Research results: there was determined the dose-dependent increase of the spontaneous level of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers. High values of the parameter were established in the subgroup of Chornobyl clean-up workers, irradiated in the diapason of doses as 500-1000 mSv. Maximal values of Cyclin D1 level in PB lymphocytes were observed in Chornobyl clean-up workers at exacerbation of the bronchial-pulmonary pathology, bronchial asthma in anamnesis and in reconvalescents of acute radiation sickness with the radiation doses D ≥ 500 mSv. After mitogen stimulation of lymphocytes, there was noted the decrease of Cyclin D1 level in the group of Chornobyl clean-up workers and the increase in persons of the control group.

Conclusions: the research revealed the differences in products of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers and persons of the control group.  The revealed changes of the spontaneous and mitogen-induced levels of Cyclin D1 in PB lymphocytes of Chornobyl clean-up workers with the somatic pathology reflect disorders in regulation processes of proliferation and cellular cycle. The obtained data add ideas about mechanisms of the radiation-induced disorder of the cellular cycle that may be a manifestation of genome instability and become a trigger factor of carcinogenesis in the remote period after radiation exposure

Author Biographies

Liliia Zvarych, State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050

Laboratory Assistant

Laboratory of Immunocytology

Institute of Clinical Radiology

Nataliya Golyarnik, State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050

PhD, Senior Researcher

Laboratory of Immunocytology

Institute of Clinical Radiology

Iryna Ilienko, State Institution "National Research Center for Radiation Medicine of National Academy of Medical Sciences of Ukraine" Yuriia Illienko str., 53, Kyiv, Ukraine, 04050

Doctor of Biological Sciences, Head of Laboratory

Laboratory of Immunocytology

Institute of Clinical Radiology

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Published

2019-07-11

How to Cite

Zvarych, L., Golyarnik, N., & Ilienko, I. (2019). Level of Cyclin D1 protein in peripheral blood lymphocytes of Chornobyl clean-up workers in remote period after radiation exposure. ScienceRise: Biological Science, (2 (17), 4–9. https://doi.org/10.15587/2519-8025.2019.165703

Issue

No. 2 (17) (2019)

Section

Biological Sciences

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Copyright (c) 2019 Liliia Zvarych, Nataliya Golyarnik, Iryna Ilienko

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