Investigation of the influence of dry extracts of bupleurum aureum and Salsola collina L. on the antimicrobial effect of co-trimoxazole

Authors

DOI:

https://doi.org/10.15587/2519-8025.2023.275942

Keywords:

pediatrics, co-trimoxazole, phytoextracts, hepatoprotectors, antimicrobial action, combined drugs

Abstract

Scientific data on the pharmacodynamics of dry extracts of Bupleurum aureum and Salsola collina L. based on the results of studying the antimicrobial effect and the similar effect of co-trimoxazole when they are used together have been supplemented. The investigated phytoextracts do not show antimicrobial properties, but they do not change the antimicrobial effect of co-trimoxazole when they are used in combination.

The aim of the study was to experimentally investigate the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establish the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when used together.

Materials and methods. The research was conducted in May 2016. Screening of the antimicrobial effect of extracts of Bupleurum aureum and Salsola collina L. and establishing of the possible antagonistic effect of these extracts on the antimicrobial drug co-trimoxazole when they are used together was carried out in the laboratory of the Department of Microbiology of the National Pharmaceutical University, which has a certificate of attestation 045/14 dated 28.10.2014. For determination of antimicrobial activity, the agar diffusion method ("well" method), which is based on the ability of medicinal substances to penetrate the agar layer, was used. A set of reference strains of microorganisms was used: S. aureus ATCC 6538, E. coli ATCC 8739, P. aeruginosa ATCC 9027, B. subtilis ATCC 6633, C. albicans ATCC 10231. Petri dishes were filled with two layers of solid nutrient medium. The lower layer - 10 ml of melted "cold" AGV agar (medium No. 3), the upper layer - nutrient medium for the corresponding test strain. After cooling the lower layer of agar, three thin-walled steel cylinders (inner diameter - 6.0±0.1 mm, height - 10.0±0.1 mm) were placed on it at an equal distance from each other and from the edge of the cup. The top layer was poured around the cylinders - 13.5 ml of agar, melted and cooled to 45-48°С, mixed with the seed dose of the test microorganism (1.5 ml of microbial suspension, the concentration corresponding to the type of microorganism). After cooling the upper layer of agar, the cylinders were removed with sterile tweezers and 0.25-0.3 ml of the studied drug was added to the resulting wells. The results were recorded after 24 h by measuring the zone of growth inhibition, including the diameter of the wells. Measurements were made with an accuracy of 1 mm, while focusing on the complete absence of visible growth.

The obtained data were analyzed using the methods of variational statistics. The significance level is p<0.05. The studied plant extracts of Bupleurum aureum (aqueous and alcoholic) and Salsola collina L. (aqueous and alcoholic) were used in doses of 0.005 mg/ml and 0.01 mg/ml, which corresponded to doses of 5 mg/kg and 10 mg/kg. Experimental data were also processed by parametric (Newman-Keuls) and non-parametric (Mann-Whitney) methods of variational statistics, using the Statistica 6.0 statistical software package; differences were considered statistically significant at p<0.05.

Results. At the final stage, the determination of the antimicrobial effect of water and alcohol extracts of Bupleurum aureum and Salsola collina L. at doses of 1 mg/ml and 5 mg/ml was carried out, and the effect of BAS of these extracts on the antimicrobial effect of co-trimoxazole when used together was determined. In the course of the study, it has been established, that the addition of the above-mentioned extracts to the co-trimoxazole formulation does not affect its initial antimicrobial properties.

Conclusion. Today, drug-induced liver injury remains one of the most important problems of hepatology and pediatrics, pharmacological science pays a lot of attention to the search for new effective and harmless drugs with a hepatoprotective effect, and the improvement of existing drugs is primarily aimed at increasing their specificity and reducing side effects related to the pharmacological properties of the drug. Currently, there is increasing interest in medicinal plants as a source of various biologically active substances (BAS), which provide a wide spectrum of pharmacological action of the agent, which allows to immediately affect various links of the pathogenesis of liver diseases. The analysis of scientific sources made it possible to establish that medicinal products of plant origin, thanks to BAS, possess polymodality of effects and reveal a versatile complex effect on the course of pathological processes in the body. Most drugs are characterized by good tolerability, absence of withdrawal syndrome and toxicity to parenchymal organs. Medicinal plants are used not only as monopreparations, but also in combination with synthetic drugs and as raw materials for obtaining BAS

Author Biographies

Olga Naboka, National University of Pharmacy

Doctor of Biological Sciences, Professor

Department of Biochemistry

Alla Kotvitska, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Rector

Nataliia Filimonova, National University of Pharmacy

Doctor of Medical Sciences, Professor, Head of Department

Department of Microbiology, Virology and Immunology

Alla Glushchenko, Kharkiv Medical Academy of Postgraduate Education

PhD, Associate Professor

Department of Clinical Laboratory Diagnostics

Olga Filiptsova, National University of Pharmacy

Doctor of Biological Sciences, Professor

Department of Cosmetology and Aromology

Alina Volkova, National University of Pharmacy

PhD, Associated Professor, Head of Department

Department of Social Pharmacy

References

  1. Andrade, R. J., Tulkens, P. M. (2011). Hepatic safety of antibiotics used in primary care. Journal of Antimicrobial Chemotherapy, 66 (7), 1431–1446. doi: https://doi.org/10.1093/jac/dkr159
  2. McDonnell, M. E., Braverman, L. E., Patel, K. P. et al. (2014). Drug-related hepatotoxicity. New England Journal of Medicine, 354, 2191–2193. doi: https://doi.org/10.1056/nejmc060733
  3. Chekman, I. S., Matveeva, E. V., Zagorodnyi, M. I., Grebelnik, A. I., Replianchuk, N. D. (2014). «Polza-risk» v farmakoterapii: sostoianie problemy, puti k razresheniiu. Ratcionalnaia farmakoterapiia, 4 (33), 5–9.
  4. Xenobiotic-Metabolizing Cytochrome P-450 (2013). Reports of Morphology, 19 (1), 41–49.
  5. Chetli, E. (1998). Problemnye lekarstva. Available at: https://www.klex.ru/6rq
  6. Clajus, C., Kühn-Velten, W. N., Schmidt, J. J., Lorenzen, J. M., Pietsch, D., Beutel, G., Kielstein, J. T. (2013). Cotrimoxazole plasma levels, dialyzer clearance and total removal by extended dialysis in a patient with acute kidney injury: risk of under-dosing using current dosing recommendations. BMC Pharmacology and Toxicology, 14 (1), 14–19. doi: https://doi.org/10.1186/2050-6511-14-19
  7. Salvo, F., De Sarro, A., Caputi, A. P., Polimeni, G. (2009). Amoxicillin and amoxicillin plus clavulanate: a safety review. Expert Opinion on Drug Safety, 8 (1), 111–118. doi: https://doi.org/10.1517/14740330802527984
  8. Derzhavnyi reiestr likarskykh zasobiv Ukrainy. Available at: http://www.drlz.com.ua/ibp/ddsite.nsf/all/index?opendocument
  9. Portal poshuku ta broniuvannia farmatsevtychnoi produktsii v Ukraini Tabletki.ua. Available at: https://tabletki.ua/uk/substance/739/
  10. Dovidnyk likarskykh zasobiv «Kompendium». Available at: https://compendium.com.ua/uk/
  11. Vasileva, O. A., Glushchenko, A. V., Georgiiantc, V. A., Garnaia, S. V. (2009). Tcelesoobraznost razrabotki kombinirovannykh lekarstvennykh sredstv v pediatricheskoi praktike. Farmatciia Kazakhstana: integratciia nauki, obrazovaniia i proizvodstva. Kazakhstan, Tashkent, 2, 288–289.
  12. Husby, S., Koletzko, S., Korponay-Szabó, I. R., Mearin, M. L., Phillips, A., Shamir, R. et al. (2012). European Society for Pediatric Gastroenterology, Hepatology, and Nutrition Guidelines for the Diagnosis of Coeliac Disease. Journal of Pediatric Gastroenterology & Nutrition, 54 (1), 136–160. doi: https://doi.org/10.1097/mpg.0b013e31821a23d0
  13. Abusin, S., Johnson, S. (2008). Sulfamethoxazole/Trimethoprim induced liver failure: a case report. Cases Journal, 1 (1), 44–46. doi: https://doi.org/10.1186/1757-1626-1-44
  14. Tatochenko, V. K. (2017). Pobochnye deistviia lekarstv v pediatricheskoi praktike. Zdorove Ukrainy, 5 (1), 64–66.
  15. Mujahid, H., Fouzia, H., Baqar, S. et al. (2016). Screening of antibacterial activity of different brands of co-trimoxazole paediatric suspension. Pakistan Journal of Pharmacology, 23 (2), 37–40.
  16. Kurkin, V. A. (2012). The flavonoids as the bioantioxidants. Polyphenols Communications 2012: 21 International conferences on polyphenols Marrakech-Morocco, 285–286.
  17. Huang, W., Zhao, Y., Sun, R. (2011). Research development on hepatoprotective effect and hepattoxicity based on bupleurum saikosaponin components. Chinese Journal of Pharmcovigilance, 8, 38–40.
  18. Chiang, L. C., Ng, L. T., Liu, L. T., Shieh, D. E., Lin, C. C. (2003). Cytotoxicity and Anti-Hepatitis B Virus Activities of Saikosaponins fromBupleurumSpecies. Planta Medica, 69 (8), 705–709. doi: https://doi.org/10.1055/s-2003-42797
  19. Wang, B.-J., Liu, C.-T., Tseng, C.-Y., Wu, C.-P., Yu, Z.-R. (2004). Hepatoprotective and antioxidant effects of Bupleurum kaoi Liu (Chao et Chuang) extract and its fractions fractionated using supercritical CO2 on CCl4-induced liver damage. Food and Chemical Toxicology, 42 (4), 609–617. doi: https://doi.org/10.1016/j.fct.2003.11.011
  20. Law, B. Y.-K., Mo, J.-F., Wong, V. K.-W. (2014). Autophagic effects of Chaihu (dried roots of Bupleurum Chinense DC or Bupleurum scorzoneraefolium WILD). Chinese Medicine, 9 (1). doi: https://doi.org/10.1186/1749-8546-9-21
  21. Naboka, O. I., Khuari, S. Z., Koshova, O. Yu., Hlushchenko, A. V. (2014). Doslidzhennia farmakodynamiky vodnoho ta spyrtovoho ekstraktiv laskavtsia zolotystoho. Klinichna farmatsiia, 4 (18), 58–62.
  22. Naboka, О. І., Khouari, S. Z., Koshova, O. Iu. (2015). Study of ethanolic and water extracts of Bupleurum Aureum on spontaneous hemolysis under the method Jager F. S. Topical issues of new drugs development. Kharkiv: NUPh, 327.
  23. Naboka, О. І., Khouari, S. Z., Koshova, O. Iu. (2015). Definition of antiexudative activity of water and ethanolic extracts of Salsola Collina Pall. on a model of zymosan-induced edema of paws in rats. Topical issues of new drugs development. Kharkiv: NUPh, 326.
  24. Naboka, O. I., Khuari, S. Z., Koshova, O. Yu., Hlushchenko, A. V. (2014). Morfolohichne doslidzhennia mozhlyvoi hepatotoksychnoi dii Bi-tolu ta koryhuiuchoho vplyvu ekstraktiv laskavtsia zolotystoho ta kuraiu pahorbkovoho u porivnianni z syliborom i kvertsetynom. Ukrainskyi biofarmatsevtychnyi zhurnal, 6 (35), 64–73.
  25. Samer, K., Glushenko, A. V., Georgiyants, V. A., Valyhura, Iu., Naboka, O. I. (2015). Morphological examination of possible pancreotoxic and immunmodulatory action of Co-trimoxazole and corrective action on these effects of extracts of Bupleurum aureum and Hill-growing Saltwort Herb in comparison with Siliborum and Quercetin. Journal of Chemical and Pharmaceutical Research, 6 (7 (2)), 952–964.
  26. Volianskyi, Yu. L., Shyrobokov, V. P., Biriukova, S. V. et al. (2004). Vyvchennia spetsyfichnoi aktyvnosti protymikrobnykh likarskykh zasobiv. Kyiv, 38.
  27. Lapach, S. N., Chubenko, A. V., Babich, P. N. (2001). Statisticheskie metody v mediko-biologicheskikh issledovaniiakh s ispolzovaniem Excel. Kyiv: Morin, 320.
  28. Chekman, I. S., Gorchakova, N. O., Panasenko, N. I., Bekh, P. O. (2006). Pharmacology. Vinnytsya: NOVA KNYHA Publishers, 384.
  29. Andrade, R., Lucena, M., Fernandez, M., Pelaez, G., Pachkoria, K., Garciaruiz, E. et al. (2005). Drug-Induced Liver Injury: An Analysis of 461 Incidences Submitted to the Spanish Registry Over a 10-Year Period. Gastroenterology, 129 (2), 512–521. doi: https://doi.org/10.1016/j.gastro.2005.05.006
  30. Chang, C. Y., Schiano, T. D. (2007). Review article: drug hepatotoxicity. Alimentary Pharmacology & Therapeutics, 25 (10), 1135–1151. doi: https://doi.org/10.1111/j.1365-2036.2007.03307.x
  31. Thiim, M., Friedman, L. S. (2003). Hepatotoxicity of antibiotics and antifungals. Clinics in Liver Disease, 7 (2), 381–399. doi: https://doi.org/10.1016/s1089-3261(03)00021-7

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Published

2023-03-31

How to Cite

Naboka, O., Kotvitska, A., Filimonova, N., Glushchenko, A., Filiptsova, O., & Volkova, A. (2023). Investigation of the influence of dry extracts of bupleurum aureum and Salsola collina L. on the antimicrobial effect of co-trimoxazole. ScienceRise: Biological Science, (1(34), 4–11. https://doi.org/10.15587/2519-8025.2023.275942

Issue

No. 1(34) (2023)

Section

Biological research

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Copyright (c) 2023 Olga Naboka, Alla Kotvitska, Nataliia Filimonova, Alla Glushchenko, Olga Filiptsova, Alina Volkova

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