The study of bone marrow cells in rats according to their age and caloric intake

Authors

DOI:

https://doi.org/10.15587/2519-8025.2017.93632

Keywords:

cells, bone marrow, age, caloric, restriction, culturing, proliferation, viability, differentiation, myelogram

Abstract

The bone marrow is a central organ of haemopoiesis and a source of pluripotent hemopoietic stem cells. The age-related changes in bone marrow induce the disorder of coordination between parenchyma and stroma of the organ that negatively influences proliferative potential and differentiation of the cells. That is why it is urgent to study the age-related changes of bone marrow and influence of caloric intake limitation on it.

Aim. The aim of presented work was the study of proliferative potential, index of viability and percentage ratio of the bone marrow cells in rats depending on their age and caloric intake.

Methods. The following methods were used in the study: selection and cultivation of the bone marrow stem cells, assessment of proliferative potential and viability of cells, analysis of percentage ratio of cellular types.

Result. It was established, that the number of cells in bone marrows increases with age. The limitation of caloric intake favors the decrease of cells number in bone marrow despite the rats’ age. The received data demonstrated that the limitation of caloric intake increases the proliferative potential of the bone marrow only in old animals. It was established, that the viability of bone marrow cells was high during all period of cultivation. The morphological population of bone marrow cells in rats of different age was heterogenic, not depending on the conditions of their feeding. It was demonstrated, that the limitation of feeding caused the increase of the number of non-differentiated blasts in bone marrow of old animals that can be connected with the activation of the processes of self-activation of stem cells pool and non-differentiated predecessors of immune system cells. The morphological analysis of the bone marrow cells culture of all animal groups despite the age and nutrition regime demonstrated that the cultivation favors the selective decrease of cells heterogeneity. At that the cells of erythroid and megakaryocytic sprouts were absent in cellular cultures.

Conclusions. As a result, the limitation of caloric intake of old rats favors the restoration or activation of proliferative potential of immunocompetent cell of bone marrow in vitro system

Author Biography

Natalia Kolot, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraine, 61022

PhD, Associate Professor

Department of Genetics and Cytology

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Published

2017-02-28

How to Cite

Kolot, N. (2017). The study of bone marrow cells in rats according to their age and caloric intake. ScienceRise: Biological Science, (1 (4), 9–13. https://doi.org/10.15587/2519-8025.2017.93632

Issue

No. 1 (4) (2017)

Section

Biological Sciences

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Copyright (c) 2017 Наталья Владимировна Колот

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