Study of superoxide- and NO-dependent protective mechanisms of N-acetylcysteine and losartan in ratʼs aorta and liver under streptozoticin-induced type 1 diabetes mellitus

Authors

DOI:

https://doi.org/10.15587/2519-4852.2017.119490

Keywords:

streptozoticin, superoxide, aorta, liver, rats, N-acetylcysteine, losartan

Abstract

Aim. The aim of the research was to investigate superoxide- (SR) and NO-dependent mechanisms of action of N-acetylcysteine (NAC) and losartan (LOS) in aorta and liver of rats with type 1 diabetes mellitus (DMI).

Methods. Diabetic rats were treated with NAC (1.5g/kg), LOS (20mg/kg) or combination (NAC+LOS) for 4 weeks.

Results. The rate of SR generation by mitochondria of aorta in untreated diabetic animals was significantly higher than in control, while the level of NO was decreased. Production of SR and NO in the liver of diabetic rats were dramatically increased. The marker of oxidatively damaged DNA, 8-oxoG, was raised in urine of diabetic rats. All of pharmacological schemes showed significant decrease the rate of SR generation by mitochondria of aorta and liver; NO level was lowered in the liver tissue compared to DMI. Only NAC significantly restored NO level in aorta of diabetic rats. Intervention with NAC / LOS or NAC+LOS increased the level of 8-oxoG in compare to DM1.

Conclusion. In conclusion, treatment with NAC and LOS or combination is associated with protection of aorta and liver cells in diabetic animals against toxic action of SR, preventing mitochondrial dysfunction and further DNA damage, which point out the cardioprotective effects and overall metabolic improvement

Author Biographies

Inna Sytnyk, Bogomolets National Medical University T. Shevchenka blvd., 13, Kyiv, Ukraine, 01601

Postgraduate student

Department of Clinical Pharmacology and Clinical Pharmacy

Anatoliy Burlaka, R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine Vasylkivska str., 45, Kyiv, Ukraine, 03022

Doctor of Biological Sciences, Senior Researcher

Laboratoryof metastatic microenvironment problems

Anastasia Vovk, R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of National Academy of Sciences of Ukraine Vasylkivska str., 45, Kyiv, Ukraine, 03022

PhD, Senior Engineer

Laboratoryof metastatic microenvironment problems 

Mykola Khaitovych, Bogomolets National Medical University T. Shevchenka blvd., 13, Kyiv, Ukraine, 01601

MD, Professor, Head of Department

Department of Clinical Pharmacology and Clinical Pharmacy

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2017-12-31

How to Cite

Sytnyk, I., Burlaka, A., Vovk, A., & Khaitovych, M. (2017). Study of superoxide- and NO-dependent protective mechanisms of N-acetylcysteine and losartan in ratʼs aorta and liver under streptozoticin-induced type 1 diabetes mellitus. ScienceRise: Pharmaceutical Science, (6 (10), 25–31. https://doi.org/10.15587/2519-4852.2017.119490

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No. 6 (10) (2017)

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Pharmaceutical Science

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Copyright (c) 2018 Inna Sytnyk, Anatoliy Burlaka, Anastasia Vovk, Mykola Khaitovych

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