Nanobiotechnological obtaining of liposomal forms of antioxidant preparations based on bioflavonoids

Authors

  • Daria Pylypenko National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-4727-0476
  • Vitaliy Prokhorov NanoMedTech LLC Antonovycha str., 68, Kyiv, Ukraine, 03680, Ukraine
  • Olexander Dudnichenko Kharkiv Medical Academy of Postgraduate Education Amosova str. 58, Kharkiv, Ukraine, 61176, Ukraine https://orcid.org/0000-0003-1933-8522
  • Yuriy Krasnopolsky National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0003-3469-5827

DOI:

https://doi.org/10.15587/2519-4852.2019.188679

Keywords:

hydrophobic antioxidants, bioflavonoids, curcumin, quercetin, nanobiotechnology, liposomes, method for obtaining liposomes

Abstract

Most pathological conditions are accompanied by lipid peroxidation and accumulation of oxidative stress products. The antioxidant action of natural hydrophobic compounds, such as quercetin, ubiquinone, curcumin, vitamin E, etc. is established. It is also known that these biologically active compounds act on different parts of antioxidant system. However, their use in parenteral drugs is difficult taking into account their hydrophobicity. Nanoparticles, such as liposomes, are used to increase the bioavailability of lipophilic antioxidants and to create water-soluble form of them.

The aim of the work is to develop the liposomal preparation with co-encapsulation of two hydrophobic antioxidants, namely curcumin and quercetin.

Methods. Technological methods of obtaining liposomes and analytical physicochemical, chromatographic (HPLC, TLC, GLC), methods of determination of particle size, pH were used.

Results. As a result of the study, the formulation and technology of obtaining the liposomal form of curcumin and its composition with quercetin were proposed. The effect of fatty acid composition of lipids, the ratio “lipid: active substance" and the technological conditions on the liposomes formation and the level of encapsulation of active pharmaceutical ingredients were studied. The dependence of nanoparticle sizes on the pressure value and the number of homogenization cycles was investigated. The lyophilized product with a level of encapsulation of hydrophobic antioxidants at least 85 % was obtained. The physicochemical properties of the samples were observed.

Conclusions. The technological scheme for obtaining of сomplex preparation containing curcumin and quercetin, involving the obtaining of lipid film, hydration of components, high-pressure homogenization, sterile filtration and lyophilization is proposed

Author Biographies

Daria Pylypenko, National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002

Postgraduate Student

Department of Biotechnology, Biophysics and Analytical Chemistry

Vitaliy Prokhorov, NanoMedTech LLC Antonovycha str., 68, Kyiv, Ukraine, 03680

Main Technologist

Olexander Dudnichenko, Kharkiv Medical Academy of Postgraduate Education Amosova str. 58, Kharkiv, Ukraine, 61176

MD, Professor, Head of Department

Department of Oncology and Pediatric Oncology

Yuriy Krasnopolsky, National Technical University "Kharkiv Polytechnic Institute" Kyrpychova str., 2, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Biotechnology, Biophysics and Analytical Chemistry

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Published

2019-12-30

How to Cite

Pylypenko, D., Prokhorov, V., Dudnichenko, O., & Krasnopolsky, Y. (2019). Nanobiotechnological obtaining of liposomal forms of antioxidant preparations based on bioflavonoids. ScienceRise: Pharmaceutical Science, (6 (22), 11–15. https://doi.org/10.15587/2519-4852.2019.188679

Issue

No. 6 (22) (2019)

Section

Pharmaceutical Science

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Copyright (c) 2019 Daria Pylypenko, Vitaliy Prokhorov, Olexander Dudnichenko, Yuriy Krasnopolsky

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