Development of the method for estimating complex formation using the electrochemical impedance spectroscopy on the example of the doxycycline and iron (III) interaction

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.225689

Keywords:

electrochemical impedance spectroscopy, complexation, antibiotics, drug interaction, doxycycline hyclate

Abstract

The aim. To develop an EIS method for study the interaction between medicinal products and metal salts on the example of the Doxycycline and iron (III) interaction.

Materials and methods. Measurements of the total impedance of the studied solutions have been performed using a vector circuit analyzer ZNB40 (Rohde & Schwarz, Germany). The calculations of electrical models were performed using the software package EC-Lab V10.40. Measurement cell was made of Teflon, 1 ml of volume, had 2 parallel nickel plated steel electrodes with diameter 6 mm, distance between electrodes is 9 mm. Basic electrical elements of model circuit were calculated according to type of electrochemical process that were described by Nyquist plot (RW, Rct, RS, Cd, CS etc.). Solutions were prepared immediately before the measurement. Measurements were performed at a temperature of 296±3 K. 6 control solutions of doxycycline and 6 control solutions of iron (III) chloride were prepared and measured. 11 study solutions at a molar ratio 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1 were prepared and measured. Concentration of the solutions was X∙10-3 mol/L respectively.

Results. EIS analysis of Nyquist curves of study solutions in the range of molar ratios 1: 6, 1: 5, 1: 4, 1: 3, 1: 2, 1: 1, 2: 1, 3: 1, 4: 1, 5: 1, 6: 1 showed a change in the dynamics of electrical resistance at a ratio of 1:1. In the aqueous solution at T = 296±3 K the constant formation of the solution of doxycycline hyclate and iron (III) chloride is 2.9. This value of the complexation constant indicates that doxycycline hyclate forms a stable metal-ligand complex with iron (III) ions.

Conclusions. EIS method can be applied to study the interaction of medicinal products. Model of this study was created on the example of doxycycline hyclate and iron (III) chloride. Metal-ion complexation of these two molecules was once again confirmed by using the EIS method

Author Biographies

Anna Dobrova, National University of Pharmacy

Postgraduate student

Department of Pharmaceutical Chemistry

Yevhenii Antonenko, V. N. Karazin Kharkiv National University

Researcher, Senior lecturer

Department of Physical and Biomedical Electronics and Complex Information Technologies

Olga Golovchenko , National University of Pharmacy

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Natalia Harna, National University of Pharmacy

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Svitlana Garna , National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Quality, Standardization and Certification of Medicines

Victoriya Georgiyants , National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Chemistry

References

  1. Rana, D., Suthar, J., Malhotra, S., Patel, V., Patel, P. (2014). A study of potential adverse drug-drug interactions among prescribed drugs in medicine outpatient department of a tertiary care teaching hospital. Journal of Basic and Clinical Pharmacy, 5 (2), 44–48. doi: http://doi.org/10.4103/0976-0105.134983
  2. Selvarajan, S., Das, S., Behera, S., Xavier, A., Dharanipragada, S. (2019). Are drug-drug interactions a real clinical concern? Perspectives in Clinical Research, 10 (2), 62–66. doi: http://doi.org/10.4103/picr.picr_55_18
  3. Robertson, S., Penzak, S. R., Huang, S. M. (2012). Chapter 15 – Drug Interactions. Principles of Clinical Pharmacology. Academic Press, 239–257. doi: http://doi.org/10.1016/b978-0-12-385471-1.00015-5
  4. Dobrova, A. O., Materiienko, A. S., Golovchenko, O. S., Georgiyants, V. A. (2017). The biopharmaceutical study of doxycycline interaction with mineral waters and soft drinks in vitro. Clinical Pharmacy, 21 (3), 55–62. doi: http://doi.org/10.24959/cphj.17.1434
  5. Dobrova, A. O., Golovchenko, O. S., Georgiyants, V. A. (2021). Modelling and investigation of amoxicillin chemical interaction with mineral waters containing a significant amount of calcium and magnesium salts. Pharmacia, 68 (1), 101–107. doi: http://doi.org/10.3897/pharmacia.68.e39573
  6. Lasia, A. (2014). Electrochemical Impedance Spectroscopy and its Applications. New York: Springer, 367. doi: http://doi.org/10.1007/978-1-4614-8933-7
  7. Barsoukov, E., Macdonald, J. R. (2018) Impedance Spectroscopy: Theory, Experiment, and Applications. John Wiley & Sons, Inc. doi: http://doi.org/10.1002/9781119381860
  8. Kozheshkurt, V., Antonenko, Ye., Shtoda, D., Slipchenko, O., Katrych, V. (2018). Possibilities of Impedance Spectroscopy for the Study of Bioliquids 2018. 9th International Conference IEEE, 260–263 doi: http://doi.org/10.1109/uwbusis.2018.8520236
  9. Selwin Joseyphus, R., Viswanathan, E., Justin Dhanaraj, C., Joseph, J. (2012). Dielectric properties and conductivity studies of some tetradentate cobalt(II), nickel(II), and copper(II) Schiff base complexes. Journal of King Saud University – Science, 24 (3), 233–236. doi: http://doi.org/10.1016/j.jksus.2011.03.004
  10. Khan, F. (2007). Electrochemical study and dependence of 'transition state' in Co(II) and Ni(II) complexes with some antibiotics and cephalothin. Eclética Química, 32 (3), 73–83. doi: http://doi.org/10.1590/s0100-46702007000300010
  11. Jenkins, A. J., Valentine, J. L.; Mozayani, A., Raymon, L. (Eds.) (2012). Antimicrobial Drugs. Handbook of Drug Interactions. Humana Press, 385–411. doi: http://doi.org/10.1007/978-1-61779-222-9_10
  12. Lambs, L., Brion, M., Berthon, G. (1984). Metal ion-tetracycline interactions in biological fluids. Part 3. Formation of mixed-metal ternary complexes of tetracycline, oxytetracycline, doxycycline and minocycline with calcium and magnesium, and their involvement in the bioavailability of these antibiotics in blood plasma. Agents and Actions, 14 (5-6), 743–750. doi: http://doi.org/10.1007/bf01978919
  13. Guerra, W., Silva, I. R., Azevedo, E. A., Monteiro, A. R. de S., Bucciarelli-Rodriguez, M., Chartone-Souza, E. et. al. (2006). Three new complexes of platinum(II) with doxycycline, oxytetracycline and chlortetracycline and their antimicrobial activity. Journal of the Brazilian Chemical Society, 17 (8), 1627–1633. doi: http://doi.org/10.1590/s0103-50532006000800021
  14. State Pharmacopoeia of Ukraine (2015). Kharkiv: State Enterprise: “Scientific-and-expert Pharmacopeial Centre”, 1128.
  15. Bard, A. J., Faulkner, L.R. (2001). Electrochemical methods: fundamentals and applications. John Wiley & Sons, Inc., 864.
  16. Tao, Z., Liu, G., Li, Y., Zhang, R., Su, H., Li, S. (2020). Electrochemical Investigation of Tetrazolium Violet as a Novel Copper Corrosion Inhibitor in an Acid Environment. ACS Omega, 5 (9), 4415–4423. doi: http://doi.org/10.1021/acsomega.9b03475
  17. Christy, F. A., Shrivastav, P. S. (2011). Conductometric Studies on Cation-Crown Ether Complexes: A Review. Critical Reviews in Analytical Chemistry, 41 (3), 236–269. doi: http://doi.org/10.1080/10408347.2011.589284
  18. Khammas, Z. A. A., Rashid, R. A. (2016). Visible spectrophotometric analysis for the mutual determination of doxycycline hydrochloride and iron in real samples after cloud point extraction. International Journal of Chemical Science, 14 (2), 955–977.
  19. Javed, J., Zahir, E. (2017). Thermodynamic studies of iron chelation with doxycycline in acidic medium. Russian Journal of Physical Chemistry A, 91 (6), 1045–1050. doi: http://doi.org/10.1134/s0036024417060115

Downloads

Published

2021-02-27

How to Cite

Dobrova, A. ., Antonenko, Y. ., Golovchenko , O. ., Harna, N. ., Garna , S. ., & Georgiyants , V. . (2021). Development of the method for estimating complex formation using the electrochemical impedance spectroscopy on the example of the doxycycline and iron (III) interaction . ScienceRise: Pharmaceutical Science, (1 (29), 31–38. https://doi.org/10.15587/2519-4852.2021.225689

Issue

No. 1 (29) (2021)

Section

Pharmaceutical Science

License

Copyright (c) 2021 Анна Олегівна Доброва, Євгеній Олександрович Антоненко, Ольга Сергеевна Головченко, Наталія Василівна Гарна , Світлана Василівна Гарна , Вікторія Акопівна Георгіянц

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.