Phytochemical research and anti-inflammatory activity of the dry extracts from northern highbush blueberry leaves




northern highbush blueberry, dry extract, phenolic compounds, amino acid, anti-inflammatory activity


All over the world, non-steroidal anti-inflammatory drugs (NSAIDs) are taken annually by about three hundred million people and this figure is constantly increasing. At the same time, NSAIDs are also one of the most common causes of side effects of drug therapy. The development and implementation of new anti-inflammatory drugs, including those of plant origin, with minimal side effects is an urgent task of modern pharmaceutical science. Vaccinium corymbosum L. (family Ericaceae), which is gaining more and more popularity among berry crops and is successfully cultivated in Ukraine, is promising in this direction for research.

The aim: phytochemical analysis of dry extracts from blueberry leaves to establish the possibility of creating new drugs with anti-inflammatory activity.

Materials and methods. The objects of the study were dry extracts of northern highbush blueberry leaves. The content of amino acids and phenolic compounds was determined by HPLC and spectrophotometry. The prototypal activity was studied in vivo and in vitro.

Research results. 4 dry extracts were obtained from northern highbush blueberry leaves. In the extracts obtained by HPLC, 7 amino acids were identified, including 3 essential ones: arginine, histidine, and phenylalanine. As a result of the HPLC study, 7 phenolic compounds were identified in extracts from the leaves of northern highbush blueberry: 5 flavonoids - rutin, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, quercetin and kaempferol and 2 hydroxycinnamic acids, chlorogenic and caffeic acid. For the first time, the anti-inflammatory effect of extracts from blueberry leaves was investigated. It was revealed that extract 1 at a dose of 50 mg/kg and extract 4 modified with arginine at a dose of 25 mg/kg have the highest anti-inflammatory activity.

Conclusions. The results of the conducted studies indicate that extracts from the leaves of northern highbush blueberry in terms of the content of biologically active substances are promising sources for the creation of new drugs and dietary supplements with anti-inflammatory activity

Author Biographies

Oleksandr Stremoukhov , National University of Pharmacy

Postgraduate Student

Department of Pharmacognosy

Oleh Koshovyi, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmacognosy

Mykola Komisarenko , National University of Pharmacy

PhD, Assistant

Department of Pharmacognosy

Igor Kireyev, National University of Pharmacy

MD, Professor, Director

Educational and Scientific Institute of Applied Pharmacy

Andriy Gudzenko, Private Higher Educational Establishment «Kyiv Medical University»

Doctor of Pharmaceutical Sciences, Head of Department

Department of Chemistry

Olha Mykhailenko , National University of Pharmacy

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Michal Korinek, Kaohsiung Medical University; Chang Gung University


Graduate Institute of Natural Products

College of Pharmacy

Department of Biotechnology

College of Life Science

Graduate Institute of Natural Products

College of Medicine

Tsong-Long Hwang, Chang Gung University; Chang Gung University of Science and Technology; Chang Gung Memorial


Graduate Institute of Natural Products

College of Medicine

Research Center for Chinese Herbal Medicine

Research Center for Food and Cosmetic Safety

Institute of Health Industry Technology

College of Human Ecology

Department of Anesthesiology

Meng-Hua Chen, Chang Gung University


Graduate Institute of Natural Products

College of Medicine


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How to Cite

Stremoukhov , O., Koshovyi, O., Komisarenko , M. ., Kireyev, I. ., Gudzenko, A. ., Mykhailenko , O., Korinek, M., Hwang, T.-L., & Chen, M.-H. (2021). Phytochemical research and anti-inflammatory activity of the dry extracts from northern highbush blueberry leaves. ScienceRise: Pharmaceutical Science, (2 (30), 40–48.



Pharmaceutical Science