Application of approach for development of hptlc identification and quantification methods for determination of phenolic compounds and terpenoids of several Thymus L. species

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.238806

Keywords:

high-performance thin-layer chromatography, Thymus, standardization, identification, quantification, flavonoids, essential oils, triterpenoids

Abstract

The aim of this work was to improve the approaches for standardization of Thymus L. species by development of HPTLC identification of phenolic compounds (flavonoids and phenylpropanoids) and terpenoids (essential oils and triterpenoids) and quantitative determination of rosmarinic acid and flavonoids for six Thymus L. species of Ukraine flora, as well as determination of chromatographic profiles of Thymus L. species’ extracts obtained using different extraction methods.

Materials and methods. Objects were: samples of dry herb of Th.serpyllum, Th.vulgaris, Th.Marschallianus, Th.Pallasianus, Th.calcareus, and Th.moldavicus of Ukraine origin collected in 2019-2021 years or acquired commercially on Ukraine market. Instruments: CAMAG HPTLC Herbal System, visionCats 2.5. The analytical grade reagents were used. Reference substances were purchased from Extrasynthese, Sigma Aldrich. Chromatography was performed on HPTLC plates Si 60 F254, Merck according to the developed methods.

Results. A new approach for quality control of different Thymus L. species of Ukraine flora included the development of HPTLC methods for identification of main groups of bioactive substances of these species, such as flavonoids and phenylpropanoids, essential oils, triterpenoids; development of quantification method of rosmarinic acid and assay of total flavonoids, expressed as luteolin-7-O-glucoside. The characteristic HPTLC fingerprints of six Thymus L. species in three mobile phases of different polarities that cover a wide range of bioactive substances were established. The content of rosmarinic acid in different Thymus L. species samples was in the range of 0,11-0,72 %: Th.moldavicus – 0,11 %; Th.Marschallianus – 0,19-0,27 %; Th.serpyllum – 0,38 %; Th.vulgaris – 0,51 %; Th.calcareus – 0,56 %; T.Pallasianus – 0,72 %. The total flavonoids content, expressed as luteolin-7-O-glucoside, was in the range of 0,8-2,72 %: Th.moldavicus – 0,8 %; Th.serpyllum – 0,87 %; Th.vulgaris – 1,06 %; Th.Pallasianus – 1,28 %; Th.Marschallianus – 1,89 %; Th.calcareus – 2,72 %.

Conclusions. The proposed scientific approach for quality evaluation of Thymus L. species using HPTLC allows to determine comprehensive information of chemical composition and content of active substances of multiple samples in parallel, in a cost and time-efficient manner

Author Biographies

Kateryna Khokhlova, National University of Pharmacy

PhD, Associate Professor

Department of Technology of Drugs

Liliia Vyshnevska, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Technology of Drugs

Oleksandr Zdoryk, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Quality, Standardization and Certification of Drugs

Institute of Professional Skills Improvement in Field of Pharmacy

Olga Filatova, National University of Pharmacy

PhD, Associate Professor

Department of Botany

 

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Published

2021-08-31

How to Cite

Khokhlova, K., Vyshnevska, L., Zdoryk, O., & Filatova, O. (2021). Application of approach for development of hptlc identification and quantification methods for determination of phenolic compounds and terpenoids of several Thymus L. species. ScienceRise: Pharmaceutical Science, (4(32), 29–36. https://doi.org/10.15587/2519-4852.2021.238806

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Section

Pharmaceutical Science