Development of green production technology and research of harpagophytum procumbens root dry extract
Keywords:Harpagophytum procumbens, harpagoside, standardization parameters, analgesic and anti-inflammatory activities
The aim of this work is to study of standardization parameters and pharmacological properties of Harpagophytum procumbens (H. procumbens) root dry extract.
Materials and methods – obtaining of H. procumbens root dry extract was performed in accordance with developed scheme that is characterized by simplicity and cost-effectiveness of technology compared to analogous scheme. Study of standardization parameters of obtained extract was performed in accordance with the requirements of the monograph «Harpagophyti extractum siccum» of the State Pharmacopoeia of Ukraine 2.2., harmonized with the monograph «Harpagophyti extractum siccum» of the European Pharmacopoeia 9.5. Pharmacology study of analgesic and anti-inflammatory activities were conducted on the rat paw formalin-induced edema model.
Results. Obtained H. procumbens root dry extract is a free flowing, non-hygroscopic, light brown powder with taste and odor that is specific to raw material of H. procumbens. Determined loss on drying was 3.2±0.18 %. Content of heavy metals in dry extract was not more than 0.01 % (100 ppm). Harpagoside was identified by TLC method. Chromatographic zones on the obtained chromatograms of the reference solution and test solutions are the same in intensity of a color, cross-over and sharpness of development. Based on the results of the research performed by HPLC method, it has been established that content of harpagoside in investigated H. procumbens root dry extract is 2.50±0.02 %.
According to the results of pharmacological research it was found that investigated dry extract (the content of harpagoside is not less than 37.0 mg/kg) showed a higher activity compared to reference drug («Phong Te Thap»).
Conclusions. A new method of obtaining dry extract of H. procumbens (3.5:1) was proposed. The developed green production technology is simple; it does not require special equipment and expensive solvents. A correspondence of quality parameters with the requirements of State Pharmacopoeia of Ukraine and European Pharmacopoeia: appearance, identification of harpagoside and fructose, loss of drying, content of heavy metals, assay of harpagoside (2.50±0.02 %) was set. Analgesic and anti-inflammatory activities of H. procumbens dry extract has been established
- Briggs, A. M., Woolf, A. D., Dreinhöfer, K., Homb, N., Hoy, D. G., Kopansky-Giles, D. et. al. (2018). Reducing the global burden of musculoskeletal conditions. Bulletin of the World Health Organization, 96 (5), 366–368. doi: http://doi.org/10.2471/blt.17.204891
- Global action plan for the prevention and control of noncommunicable diseases 2013–2020 (2013). World Health Organization. Available at: https://apps.who.int/iris/handle/10665/94384
- Shhorіchna dopovіd pro stan zdorovya naselennya, sanіtarno-epіdemіchnu situacіyu ta rezultati dіyalnostі sistemi oxoroni zdorovya Ukraini (2017). Kyiv: Ministry of Health Care of Ukraine, Public establishment “Ukrainian Institute of Strategic Researches MHC of Ukraine”, 516.
- Cavero, R. Y., Calvo, M. I. (2015). Medicinal plants used for musculoskeletal disorders in Navarra and their pharmacological validation. Journal of Ethnopharmacology, 168, 255–259. doi: http://doi.org/10.1016/j.jep.2015.03.078
- Bee, T. A., Liew, A. (2010). Dietary Supplements Used in Osteoarthritis. Proceedings of Singapore Healthcare, 19(3), 237–247. doi: http://doi.org/10.1177/201010581001900310
- Mobasheri, A. (2012). Intersection of Inflammation and Herbal Medicine in the Treatment of Osteoarthritis. Current Rheumatology Reports, 14 (6), 604–616. doi: http://doi.org/10.1007/s11926-012-0288-9
- Van Wyk, B.-E., Wink, M. (Eds.) (2014). Phytomedicines, Herbal drugs and Poisons. Cambridge Cambridge University Press, Briza, Kew Publishing, 304.
- Mobasheri, A., Henrotin, Y., Biesalski, H.-K., Shakibaei, M. (2012). Scientific Evidence and Rationale for the Development of Curcumin and Resveratrol as Nutraceutricals for Joint Health. International Journal of Molecular Sciences, 13 (4), 4202–4232. doi: http://doi.org/10.3390/ijms13044202
- Wink, M. (2015). Modes of Action of Herbal Medicines and Plant Secondary Metabolites. Medicines, 2 (3), 251–286. doi: http://doi.org/10.3390/medicines2030251
- Cameron, M., Gagnier, J. J., Little, C. V., Parsons, T. J., Blümle, A., Chrubasik, S. (2009). Evidence of Effectiveness of Herbal Medicinal Products in the Treatment of Arthritis. Part I: Osteoarthritis. Phytotherapy Research, 23 (11), 1497–1515. doi: http://doi.org/10.1002/ptr.3007
- Heinrich, M., Barnes, J., Prieto-Garcia, J., Gibbons, S., Williamson, E. (2012). Fundamentals of pharmacognosy and phytotherapy. Oxford: Elsevier Health Sciences, 336.
- Abdelouahab, N., Heard, C. (2008). Effect of the Major Glycosides ofHarpagophytum procumbens(Devil’s Claw) on Epidermal Cyclooxygenase-2 (COX-2) in Vitro. Journal of Natural Products, 71 (5), 746–749. doi: http://doi.org/10.1021/np070204u
- Rahimi, A., Razmkhah, K., Mehrnia, M., Mohamadnia, A., Sahebjamee, H., Salehi, S. et. al. (2016). Molecular docking and binding study of harpagoside and harpagide as novel anti-inflammatory and anti-analgesic compound from Harpagophytum procumbens based on their interactions with COX-2 enzyme. Asian Pacific Journal of Tropical Disease, 6 (3), 227–231. doi: http://doi.org/10.1016/s2222-1808(15)61019-2
- Mncwangi, N., Chen, W., Vermaak, I., Viljoen, A. M., Gericke, N. (2012). Devil's Claw – a review of the ethnobotany, phytochemistry and biological activity of Harpagophytum procumbens. Journal of Ethnopharmacology, 143 (3), 755–771. doi: http://doi.org/10.1016/j.jep.2012.08.013
- Chrubasik, J., Chrubasik, S., Roufogalis, B. (2010). Systematic review on the safety of Harpagophytum preparations for osteoarthritic or low back pain. Focus on Alternative and Complementary Therapies, 11, 12. doi: http://doi.org/10.1111/j.2042-7166.2006.tb04734.x
- Kriukova, A., Vladymyrova, I. (2015). Analytical review of modern herbal medicines used in musculoskeletal system diseases. ScienceRise, 10 (4 (15)), 24–31. doi: http://doi.org/10.15587/2313-8416.2015.52001
- State register of medicinal products of Ukraine. Available at: http://www.drlz.kiev.ua/
- Rolland, Y. (2010). No. US 12/599,146. Duval Ch. Novel method for preparing purified extracts of harpagophytum procumbens. 09.12.2010. US20100311675 A1.
- Cvjetko Bubalo, M., Vidovic, S., Radojcic Redovnikovic, I., Jokic, S. (2015). Green solvents for green technologies. Journal of Chemical Technology & Biotechnology, 90 (9), 1631–1639. doi: http://doi.org/10.1002/jctb.4668
- Abert Vian, M., Allaf, T., Vorobiev, E., Chemat, F.; Chemat, F., Vian, M. A. (Eds.) (2014). Solvent-free extraction:mythor reality? Alternative Solvents for Natural Products Extraction. doi: http://doi.org/10.1007/978-3-662-43628-8
- State Pharmacopoeia of Ukraine. Vol. 1. Kharkiv: State Enterprise «Ukrainian Scientific Pharmacopoeial Centre for Quality of Medicines», 1128.
- European Pharmacopoeia (2018). Strasbourg: European Department for the Quality of Medicines, 1334.
- Rukovodstvo po ekspertyze lekarstvennyh sredstv. Part I (2013). Moscow: Grif i K, 328.
- European convention for the protection of vertebrate animals used for experimental and other scientific purpose: Council of Europe (1986). Strasbourg, 52.
- Voloshhuk, N. I., Taran, I. V., Konyukh, S. A. (2015). Influence of hydrogen sulfide on analgesic and anti-inflammatory effects of diclofenac sodium in experiments. Bulletin of Vinnitsa National Medical University, 19 (1), 51–54.
- Rebrova, O. (2006). Statistical analysis of medical data. Application software package STATISTICA. Moscow: MediaSfera, 312.
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