Substantiation of technological parameters of the oil extract obtained from Urtica dioica roots based on the yield determination of phytosterols using capillary gas chromatography method

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.249615

Keywords:

Urtica dioica root, androgenic alopecia, oil extract, extraction parameters, phytosterols, gas capillary chromatography

Abstract

Due to the content of phytosterols, extractive preparations of Urtica dioica roots are able to show antiandrogenic effect in the case of external therapy of men and women with androgenic alopecia. Oil extracts (OE) are characterized by several advantages when applied to the skin of the scalp compared to water-alcohol extracts. For the development of OE technology from Urtica dioica roots, it is important to choose the optimal extraction parameters, which are based on the quantitative determination of phytosterols in the extractant and the studied samples of extracts.

The aim of the work is to choose the optimal parameters for obtaining OE from Urtica dioica roots based on quantitative determination of phytosterols content in experimental samples of OE by gas capillary chromatography.

Materials and methods. Objects of the research – Urtica dioica root, refined corn oil, refined sunflower oil, samples of oil extracts. Determination of phytosterol content in experimental samples was carried out by gas capillary chromatography (chromatograph “Crystal 2000”, manufacturer – research and production company “Analytics”).

Results. 5 different compounds of steroid structure (stigmasterol, β-sitosterol, etc.) were identified in sunflower oil by gas liquid chromatography, and 10 (campesterol, 2-α stigmasterol, β-sitosterol, Δ5-avenosterol, etc.) were identified in sunflower oil. The quantitative content of β-sitosterol in the sum of sterols of corn oil was significantly higher compared to the content of this substance in sunflower oil and amounted to 59.33 %. Optimal technological parameters were established considering the peculiarities of extraction with oil extractant and quantitative determination of the amount of phytosterols and β-sitosterol in experimental samples of OE. The total content of plant sterols in OE, including considering their amount in the extractant, was in the range of 7880 mg/kg; the amount of β-sitosterol was 4638 mg/kg.

Conclusion. The choice of optimal parameters for obtaining OE from UDR based on determination of phytosterol yield by gas capillary chromatography was experimentally substantiated, namely: extractant – corn oil, raw material-extract ratio – 1: 5, extraction time – 6 h, extraction method – maceration

Author Biographies

Mariana Fedorovska, Ivano-Frankivsk National Medical University

Doctor of Pharmaceutical Sciences, Аssociate Professor, Head of Department

Department of Organization and Economics of Pharmacy and Drug Technology

Inna Yarema, Ivano-Frankivsk National Medical University

Assistant

Department of Organization and Economics of Pharmacy and Drug Technology

Natalia Polovko, National University of Pharmacy

Doctor of Pharmaceutical Science, Professor

Department of Pharmaceutical Technology of Drugs

Iryna Ivanchuk, Ivano-Frankivsk National Medical University

PhD, Associate Professor

Department of Pharmacy

References

  1. Tryneeva, O. V., Safonova, E. F. (2013). The comparative characteristic of vegetable oils and the oil extracts applied in pharmacy. Chemistry of Plant Raw Material, 4, 77–82. doi: http://doi.org/10.14258/jcprm.1304077
  2. Nkachuk, O. Y., Vyshnevska, L. I., Zubchenko, T. M. (2016). The study of the effect of the critical parameters on the manufacturing process of the oil phytoextract with the hepatoprotective action. News of Pharmacy, 1 (85), 45–49. doi: http://doi.org/10.24959/nphj.16.2102
  3. Saraf, S., Jharaniya, M., Gupta, A., Jain, V., Shailendra (2014). Herbal hair cosmetics: advancements and recent findings. World Journal of Pharmaceutical Research, 2, 3278–3294.
  4. Sudheer Kumar, K., Gomathi, S., Seetarm Swamy, S. (2016). Formulation and evaluation of polyherbal hair oil – an economical cosmetic. International Journal of Advanced Research in Medical & Pharmaceutical Sciences, 1 (2), 10–14.
  5. Usha Kiran Reddy, T., Sindhu, G., Rajesh, S., Aruna, B., Sandhya Rani, K. S. (2017). Preparation and evaluation of herbal hair oil. Indo American Journal of Pharmaceutical Sciences, 4 (6), 1540–1546.
  6. Kashyap, R., Shukla, K., Mahajan, S. C., Sharma, A. (2016). Formulation and evaluation of hair oil for hair loss disorders. Journal of Medicinal Plants Studies, 4 (3), 13–17.
  7. Demchuk, M. B., Ivashkiv, Yu. I., Hroshovyi, T. A. (2012). Doslidzhennia vitchyznianoho rynku likarskykh preparativ i zasobiv likuvalnoi kosmetyky, shcho vykorystovuiutsia pry zovnishnii korektsii alopetsii. Zaporozhskyi medytsynskyi zhurnal, 3 (72), 23–25.
  8. Mank, V. V., Polonska, T. A. (2016). Vegetable oils compositionsfor cosmetic products. Naukovi pratsi NUKhT, 3 (22), 217–223.
  9. Pegova, R. A., Vorobeva, O. A., Kolchik, O. V. et. al. (2014). Rastitelnye masla. Sostav i perspektivy ispolzovaniia masla semian tykvy Cucurbita pepo v terapii (Obzor). Meditsinskii almanakh, 2, 127–134.
  10. Dulf, F. V., Unguresan, M. L., Vodna, D. C., Socaciu, C. (2010). Free and esterified sterol distribution in four romanian vegetable oil. Not. Bot. Hort. Agrobot. Cluj, 38 (2), 91–97.
  11. Kemal, M., Amar, S. (2006). Sterols and the phytosterol content in oilseed rape (Brassica napus L.). Journal of Cell and Molecular Biology, 5, 71–79.
  12. Chaurasia, N., Wichtl, M. (1987). Sterols and stearyl glycosides from Urtica dioica. Journal of Natural Products, 50, 881–885.
  13. Schöttner, M., Ganßer, D., Spiteller, G. (1997). Lignans from the Roots ofUrtica dioicaand their Metabolites Bind to Human Sex Hormone Binding Globulin (SHBG). Planta Medica, 63 (6), 529–532. doi: http://doi.org/10.1055/s-2006-957756
  14. Türkoğlu, M., Dündar, C., Sevinç, H., Kılıç, S. (2017). A comparative study to test a plant extract against Minoxidil in HaCaT cells. American Journal of Dermatology and Venereology, 6 (1), 6–10.
  15. Semwal, B. C., Agrawal, K. K., Singh K. et. al. (2011). Alopecia: switch to herbal medicine. Journal of Pharmaceutical Research and Opinion, 1 (4), 101–104.
  16. Lourith, N., Kanlayavattanakul, M. (2013). Hair loss and herbs for treatment. Journal of Cosmetic Dermatology, 12 (3), 210–222. doi: http://doi.org/10.1111/jocd.12051
  17. Nahata, A., Dixit, V. K. (2013). Evaluation of 5α-reductase inhibitory activity of certain herbs useful as antiandrogens. Andrologia, 46 (6), 592–601. doi: http://doi.org/10.1111/and.12115
  18. Pekmezci, E., Dündar, C., Türkoğlu, M. (2018). A proprietary herbal extract against hair loss in androgenetic alopecia and telogen effluvium: a placebo-controlled, single-blind, clinical-instrumental study. Acta Dermatovenerol APA, 27 (2), 51–57. doi: http://doi.org/10.15570/actaapa.2018.13
  19. Saeidnia, S., Manayi, A., Gohari, A. R., Abdollahi, M. (2014). The Story of Beta-sitosterol- A Review. European Journal of Medicinal Plants, 4 (5), 590–609. doi: http://doi.org/10.9734/ejmp/2014/7764
  20. Bondarenko, Zh. V., Likhodievskii, A. V., Slonskaia, S. V. (2017). Vliianie parametrov ekstragirovaniia na svoistva maslianogo ekstrakta. Pererabotka i upravlenie kachestvom selskokhaziaistvennoi produktsii. Minsk: BGATU, 383–385.
  21. Naraniwal, M., Kothari, V. (2012). Modern extraction methods for preparation of bioactive plant extracts. International Journal of Applied and Natural Sciences, 1 (1), 8–26.
  22. Azmir, J., Zaidul, I. S. M., Rahman, M. M., Sharif, K. M., Mohamed, A., Sahena, F. et. al. (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117 (4), 426–436. doi: http://doi.org/10.1016/j.jfoodeng.2013.01.014
  23. Tkachuk, O. Yu. (2016). Rozrobka skladu ta tekhnolohii kombinovanoho oliinoho fitopreparatu hepatotropnoi dii. Kharkiv, 165.
  24. Grankina, I. V., Pogorelov, V. I. (2009). Tekhnologiia polucheniia maslianogo ekstrakta tsvetkov barkhattsev. Zhurnal nauchnykh publikatsii aspirantov i doktorantov, 9, 89–90.
  25. Azwanida, N. N. (2015). A review in the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, 4 (3), 196. doi: https://doi.org/ 10.4172/2167-0412.1000196
  26. Derzhavna Farmakopeia Ukrainy. Vol. 1. Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  27. European Pharmacopoeia (2007). Strasbourg: Council of Europe, 3261.
  28. DSTU ISO 6799-2002 Zhyry ta olii tvarynni i roslynni. Vyznachannia skladu sterynovoi fraktsii. Hazokhromatohrafichnyi metod (ISO 6799:1991, IDT) (2002). Kyiv: Ukrmetrteststandart, 12.

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Published

2021-12-30

How to Cite

Fedorovska, M., Yarema, I., Polovko, N., & Ivanchuk, I. (2021). Substantiation of technological parameters of the oil extract obtained from Urtica dioica roots based on the yield determination of phytosterols using capillary gas chromatography method. ScienceRise: Pharmaceutical Science, (6 (34), 27–36. https://doi.org/10.15587/2519-4852.2021.249615

Issue

No. 6 (34) (2021)

Section

Pharmaceutical Science

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Copyright (c) 2021 Mariana Fedorovska, Inna Yarema, Natalia Polovko, Iryna Ivanchuk

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