Establishment of quality indicators of promising plant raw materials - underground organs of Rumex confertus willd

Abstract

The aim is to study the morphological and anatomical structure of the underground organs of R. confertus and to establish the type of medicinal plant raw materials, to determine a number of its indicators of quality and quantitative content of some groups of biologically active substances using modern methods of analysis.

Materials and methods. It was used air-dry and freshly collected raw materials and a microscope Delta optic BioLight 300 (Poland) to study the macro- and microscopic characteristics of the plant raw materials. Determination of amount of hydroxycinnamic acids and total polyphenols was determined spectrophotometrically according to monograph «Nettle leaf» and the method 2.8.14 of the State Pharmacopoeia of Ukraine 2.0 (on a spectrophotometer Optizen POP (Korea)). The study of the component composition of hydroxycinnamic acids was performed by HPLC.

Results. As a result of research of plant raw materials it was established that the underground organs of Rumex confertus are the roots of annual plants and the rhizomes and roots of two or three annual plants. Diagnostic features: morphological (for the root and rhizome - the nature of the surface and fracture) and anatomical (for the rhizome - aerenchyma in the cortex, the presence and location of scleroids and sclerenchyma in the plant raw materials of two or three annual plants, the presence in the cells of the cortex and pith parenchyma simple starch grains, druses and cells with yellow content in freshly harvested plant raw materials; for the root - the colour of peridermal cells, the degree of development of pith rays, the remainder of the primary xylem; a distinctive feature of the annual root from two or three annuals is the absence of scleroids). It was determined the borderline boundaries of indicators in the series of plant raw materials: loss on drying (not more than 13. 5 %), total ash (not more than 11 %), extractable matter (not less than 33 %) and the quantitative content of amount of hydroxycinnamic acids (not less than 1.3 %) and total polyphenols (not less than 3.5 %). It was identified chlorogenic and neochlorogenic acids.

Conclusions. It was determined the type of underground organs of Rumex confertus: annuals had only roots (tap root systems), from the second to the third year of life the plants have both rhizomes and tap root system. It was established their morphological and anatomical diagnostic features and determined numerical indicators and the quantitative content of the amount of hydroxycinnamic acids and total polyphenols with using modern methods of analysis and chlorogenic and neochlorogenic acids were identified. The obtained data will be used in further research, including the development of the draft monograph of the State Pharmacopoeia of Ukraine 2.0 or the draft methods of quality control of medicinal plant raw materials Rumex confertus and the creation of herbal medicines