Pharmacognostic study of cetraria islandica (L.) Ach. thalli made in Ukraine

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.265019

Keywords:

Cetraria islandica, morphological structure, anatomical structure, total polyphenols, flavonoids, carboxylic acids

Abstract

The aim was to conduct a pharmacognostic study of C. islandica thalli harvested in Ukraine.

Material and methods. The study included seven series of C. islandica thalli harvested in Ukraine. Pharmacognostic research was conducted by using generally accepted methods. Quantitative determination of total polyphenols was performed by a spectrophotometric method in terms of pyrogallol and dry raw materials following the requirements of the SPhU 2.0 monograph. The component composition of flavonoid compounds and carboxylic acids was performed by HPLC.

Results. The pharmacognostic study of 7 series of the C. islandica raw materials was performed. Morphometric parameters were determined, including the fractional composition according to the size of thalli in each series. For the first time, fractions of the mineral and foreign organic matter were separated. The content of mineral foreign matter ranged from 0.22 ± 0.01 % to 2.80 ± 0.12 %; the content of organic foreign matter ranged from 0.15 ± 0.01 % to 2.14 ± 0.11 %. Due to the total foreign matter content, 6 series of the raw materials fulfilled the requirements of the SPhU 2.0 monograph. The accordance of morphological and anatomical description of the series to the requirements of the SPhU 2.0 monograph is shown. New distinctive diagnostic features of the morphological structure were found, namely: coalescence of blades with forming a membrane and branching of cilia along the edge of blades. For the first time for the series of raw materials harvested in Ukraine, the quantitative content of the sum of polyphenols in terms of pyrogallol and dry raw materials was determined, which ranged from 1.21 ± 0.05 % to 1.73 ± 0.04 %. For the first time for C. islandica thalli, the presence of flavonoid compounds: quercetin, luteolin, kaempferol and rutin, and carboxylic acids: sinapic, trans-cinnamic and quinic acids was determined.

Conclusions. The obtained results can be used as a basis for the relevant sections of the national part of the monograph "Cetraria Iceland" in SPhU 2.0

Supporting Agency

  • National University of Pharmacy

Author Biographies

Alina Shpychak, National University of Pharmacy

Postgraduate Student

Department of Chemistry of Natural Compounds and Nutritiology

Olga Khvorost, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Chemistry of Natural Compounds and Nutritiology

References

  1. Kärnefelt, I., Mattsson, J-E., Thell, A. (1993). The lichen genera Arctocetraria, Cetraria, and Cetrariella (Parmeliaceae) and their presumed evolutionary affinities. The Bryologist, 96 (3), 394–404. doi: http://doi.org/10.2307/3243869
  2. Saag, A., Randlane, T., Saag, L., Thell, A., Ahti, T. (2013). Third world list of cetrarioid lichens: A databased tool for documentation of nomenclatural data – lessons learned. Taxon, 62 (3), 591–603. doi: http://doi.org/10.12705/623.4
  3. Passo, A., Rodriguez, J. M., Chiapella, J. O., Messuti, M. I. (2018). The Antarctic lichen Cetraria subscutata is a synonym of Nephromopsis chlorophylla. The Lichenologist, 50 (2), 239–245. doi: http://doi.org/10.1017/s0024282918000063
  4. Fungal Genera. Available at: https://www.fungalgenera.org/
  5. Index Fungorum. Available at: http://www.indexfungorum.org/
  6. MycoBank. Available at: http://www.mycobank.org/
  7. Khodosovtsev, A. Y., Darmostuk, V. V. (2020). Records of lichen species new for Ukraine from steppe habitats of the country. Botanica Serbica, 44 (2), 243–250. doi: http://doi.org/10.2298/botserb2002243k
  8. Kondratyuk, S. Ya, Popova, L. P, Lackovičova, A, Pišut, I. (2003). A Catalogue of the Eastern Carpathian Lichens. Kyiv-Bratyslava: M. H. Kholodny Institute of Botany, 264.
  9. Nadyeina, O., Lutsak, T., Blum, O., Grakhov, V., Scheidegger, C. (2013). Cetraria steppae Savicz is conspecific with Cetraria aculeata (Schreb.) Fr. according to morphology, secondary chemistry and ecology. The Lichenologist, 45 (6), 841–856. doi: http://doi.org/10.1017/s002428291300056x
  10. Printzen, C., Domaschke, S., Fernández-Mendoza, F., Pérez-Ortega, S. (2013). Biogeography and ecology of Cetraria aculeata, a widely distributed lichen with a bipolar distribution. MycoKeys, 6, 33–53. doi: http://doi.org/10.3897/mycokeys.6.3185
  11. Chervona Knyha Ukrainy. Available at: https://redbook-ua.org/item/cetraria-steppae/
  12. Derzhavnyy reyestr likarskykh zasobiv Ukrainy. Available at: http://www.drlz.com.ua/ibp/ddsite.nsf/all/shlist?opendocument
  13. Kompendium – likarski preparaty. Available at: https://compendium.com.ua/
  14. Zotsenko, L, Nuzhyna, N, Kyslychenko, V, Futorna, O. (2021) Anatomical and Ultrastructure Differences Between Some Species of the Genus Elsholtzia Willd. of Flora of Ukraine. Pharmacognosy Journal, 13(4), 977-987. doi: http://doi.org/10.5530/pj.2021.13.126
  15. Xu, M., Heidmarsson, S., Thorsteinsdottir, M., Kreuzer, M., Hawkins, J., Omarsdottir, S., Olafsdottir, E. S. (2018). Authentication of Iceland Moss (Cetraria islandica) by UPLC-QToF­MS chemical profiling and DNA barcoding. Food Chemistry, 245, 989–996. doi: http://doi.org/10.1016/j.foodchem.2017.11.073
  16. Nayaka, S., Haridas, B. (2020). Bioactive Secondary Metabolites from Lichens. Plant Metabolites: Methods, Applications and Prospects. Singapore: Springer, 255–290. doi: http://doi.org/10.1007/978-981-15-5136-9_12
  17. Studziñska-Sroka, E., Galanty, A., Bylka, W. (2017). Atranorin – an interesting lichen secondary metabolite. Mini-Reviews in Medicinal Chemistry,17, 1633–1645. doi: http://doi.org/10.2174/1389557517666170425105727
  18. Kravchenko, V. N., Georgiyants, V. A., Vladimirova, I. N., Kononenko A. G., Orlova, V.A., Shcherbak, E. A. (2014). Vliyaniye lekarstvennykh rasteniy na uroven' yodsoderzhashchikh gormonov shchitovidnoy zhelezy v krovi u krys. Biologicheskiy zhurnal Armenii, 4 (66), 17–21.
  19. Voicu, D. M. F., Mitoi, M. E., Gavriloaie, C., Helepciuc, F. E., Toma, N. (2019). Chemical investigations of lichen biomass in Usnea barbata, Cetraria islandica, and Xanthoria parietina species. Extreme Life, Biospeology & Astrobiology, 11 (1), 1–8.
  20. Goga, M., Elečko, J., Marcinčinová, M., Ručová, D., Bačkorová, M., Bačkor, M. (2020). Lichen metabolites: an overview of some secondary metabolites and their biological potential. Co-evolution of secondary metabolites. Reference Series in Phytochemistry. Springer, 175–209. doi: http://doi.org/10.1007/978-3-319-96397-6_57
  21. Nikulin, A.V., Yamshchikova, S.I., Potanina, O.G., Abramovich, R.A. (2020). Determination of usnic acid in the blastemas of a lichen of the genus Cladonia by HPLC/UV. Farmatsiya, 69 (3), 44–49. doi: http://doi.org/10.29296/25419218-2020-03-06
  22. Patriche, S., Ghinea, I. O., Adam, G., Gurau, G., Furdui, B., Dinica, R. M., Rebegea, L.-F., Lupoae, M. (2019). Characterization of Bioactive Compounds from Romanian Cetraria islandica (L) Ach. Revista de Chimie, 70 (6), 2186–2191. doi: https://doi.org/10.37358/rc.19.6.7302
  23. Igoli, J., Gray, A., Clements, C., Kantheti, P., Singla, R. (2014). Antitrypanosomal Activity & Docking Studies of Isolated Constituents from the Lichen Cetraria islandica: Possibly Multifunctional Scaffolds. Current Topics in Medicinal Chemistry, 14 (8), 1014–1021. doi: https://doi.org/10.2174/1568026614666140324122323
  24. Kosanić, M., Ranković, B. (2011). Lichen as possible sources of antioxidants. Pakistan Journal of Pharmaceutical Sciences, 24 (2), 165–170.
  25. Grujič‎ić, D., Stošić, I., Kosanić, M., Stanojković T., Ranković, B., Milošević-Djordjević, O. (2014). Evaluation of in vitro antioxidant, antimicrobial, genotoxic and anticancer activities of lichen Cetraria islandica. Cytotechnology, 66, 803–813. doi: http://doi.org/10.1007/s10616-013-9629-4
  26. Tas, I., Yildiri, A. B., Ozyigitoglu, G. C., Yavuz, M. Z., Turker, A. U. (2017). Determination of biological activities (antibacterial, antioxidant and antiproliferative) and metabolite analysis of some lichen species from Turkey. European Journal of Biomedical and Pharmaceutical Sciences, 4 (4), 13–20.
  27. Stadnytska, N., Fito, I. Novikov, V., Jasicka-Misiak, I., Wieczorek, P. P. (2020). Effect of extraction solvent on total phenolic content, total flavonoid content and antioxidant activity of Cetraria islandica. International Journal of PharmTech Research, 13 (3), 198–205. doi: http://doi.org/10.20902/ijptr.2019.130310
  28. Nikolaeva, T. N., Lapshin, P. V., Zavarzina, A. G., Zagoskina N. V. (2014). The Conjugates of Phenolic Acids in Lichens of the Order Lecanorales. International Journal of Secondary Metabolite, Special Issue-Abstracts,1 (1), 15.
  29. Yıldız, Ö., Vahapoğlu, B., Marangoz, M. A., Güven, E. Ç., Bayindirli, A. (2021). Determination of Phenolic Compound Profiles and Antioxidant Effect of Plant Extracts on Late-Release Soft Lozenge. East African Scholars Publisher Journal of Nutrition and Food Sciences, 3 (6), 167–174.
  30. Derzhavna Farmakopeia Ukrainy. Vol. 3 (2014). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 732.
  31. European Pharmacopoeia. Available at: https://pheur.edqm.eu/home
  32. British Pharmacopoeia Vol. 4 (2020). London: The Stationery Office, 902.
  33. Gosudarstvennaya farmakopeya Respubliki Belarus. Vol. 2 (2007). Minsk: Respublikanskoye unitarnoye predpriyatiye «Tsentr ekspertiz i ispytaniy v zdravookhranenii», 471.
  34. Gosudarstvennaya farmakopeya Respubliki Kazakhstan. Vol. 3 (2014). Almaty: Izdatel'skiy Dom «Zhibek zholy», 872.
  35. Gosudarstvennyy standart SSSR 13727-68 «Sloyevishcha lishaynika tsetrarii islandskoy» (1968).
  36. Vladimirova, I. M., Georgiyants, V. A., Kotov, A. G. (2013). Farmakopeyna standartyzatsiya syrovyny – tsetrariyi islandskoyi slain. Upravlinnya, ekonomika ta zabezpechennya yakosti u farmatsiyi, 1 (27), 10–13.
  37. Vladimirova, I. N., Georgiyants, V. A. (2013). Extracted compounds from Cetraria islandica. Chemistry of Natural Compounds, 49 (2), 347–348. doi: https://doi.org/10.1007/s10600-013-0601-5
  38. Sira, L. M., Vladymyrova, I. M. (2012). Morfologo-anatomichne vyvchennya talomu Cetraria islandica (L.) Ach. Fitoterapiia. Chasopys, 3, 60–62.
  39. Kobernik, A. O., Kravchenko, I. A., Chervonenko, O. F., Myhaylova, T. V., Nabych, M. (2015). The identification of active compound content in the thallus Cetraria islandica. Aktualnyye problemy transportnoy meditsiny, 2 (40), 144–148.
  40. Kovalov, V. M., Marchyshyn, S. M., Khvorost, O. P., Isakova, T. I. (2014). Praktykum z identyfikatsiyi likarskoyi roslynnoyi syrovyny. Ternopil: TDMU, “Ukrmedknyha”, 264.
  41. Derzhavna Farmakopeia Ukrainy. Vol. 1 (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  42. Justesen, U., Knuthsen, P., Leth T. (1997). Determination of plant polyphenols in Danishfood stuffs by HPLC-UV and LC-MS detection. Cancer Letters, 114 (1-2), 165–167. doi: http://doi.org/10.1016/s0304-3835(97)04651-x
  43. Seeram, N. P., Lee, R., Scheuller, H. S., Heber, D. (2006). Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization masss pectroscopy. Food Chemistry, 97 (1), 1–11. doi: http://doi.org/10.1016/j.foodchem.2005.02.047
Pharmacognostic study of cetraria islandica (L.) Ach. thalli made in Ukraine

Downloads

Published

2022-10-31

How to Cite

Shpychak, A., & Khvorost, O. (2022). Pharmacognostic study of cetraria islandica (L.) Ach. thalli made in Ukraine. ScienceRise: Pharmaceutical Science, (5 (39), 4–15. https://doi.org/10.15587/2519-4852.2022.265019

Issue

No. 5 (39) (2022)

Section

Pharmaceutical Science

License

Copyright (c) 2022 Alina Shpychak, Olga Khvorost

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.