Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Authors

DOI:

https://doi.org/10.15587/2519-4852.2023.286746

Keywords:

Rosa Damascena Mill., method of clonal micropropagation, GC-MS, antibacterial activity

Abstract

Damask rose of the Veselka variety is an important industrial rose variety used to obtain essential oil. It is widely used in modern cosmetology, perfumery, and aromatherapy. In addition, the essential oil of Damask rose has a whole spectrum of pharmacological properties.

The scientific innovation of this research lies in its foundation on cultivating and acquiring planting materials of the Damask rose in vitro. This approach guarantees controlled conditions for plant growth, the production of robust seedlings, and an enhancement in the precision and credibility of the research outcomes.

Moreover, the study has scientific novelty in that it explores to assess both the quantitative and qualitative constituents of the essential oil in the acquired plant material of the Damask rose. This assessment takes place within the context of cultivating regenerative plants in an outdoor environment. Such an approach acknowledges the potential distinctions in the oil's component composition acquired from plants propagated through this method in comparison to traditional vegetative reproduction. Lastly, the research has scientific novelty in investigating the potential antimicrobial properties of Damask rose essential oil, which could have significant practical implications in the development of new drugs and combatting infectious diseases.

The purpose of the study was to establish the component composition of the essential oil of Rosa damascena Mill., which was grown in vitro, and to determine its antimicrobial effect.

Methods. The object of the study was the essential oil of Damask rose of the Veselka variety, which was grown by the method of clonal micropropagation in vitro. The essential oil was extracted from fresh petals collected during dry weather conditions prior to sunrise by hydrodistillation. Determination of the qualitative composition and quantitative content of volatile substances was carried out by the GC-MS method using an Agilent 7890B chromatograph. Antimicrobial activity was studied in vitro using the disk diffusion method with reference test strains of microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Candida albicans ATCC 885-653.

Results. According to the results of the chromato-mass spectrometric study, 41 compounds (6 of which were in the isomeric state) were identified, which was belong to 13 different classes of chemicals. Dominant compounds among terpenoid substances were shown: geraniol – 30.96 %, citronellol – 27.08 %, alkanes: nonadecane – 17.29 %, and heneicosene – 5.46 %.

It was established that the essential oil of Damask rose had a significant antimicrobial effect against strains of C. albicans and E. coli, the diameters of which growth retardation zones ranged from 32-35 mm and 20-23 mm, respectively. In studies with P. aeruginosa and S. aureus, the essential oil showed moderate antibacterial activity: the diameters of the growth retardation zone of these microorganisms ranged from 13 to 15 mm and 11 to 12 mm, respectively.

Conclusion. For the first time, the qualitative composition and quantitative content of volatile substances in the essential oil extracted from the petals of Rosa damascena Mill., Veselka variety, cultivated through the clonal micropropagation in vitro, were explored by chromatography-mass spectrometry techniques. The results of the study of antimicrobial activity showed that the studied essential oil exhibits significant fungicidal effects against Candida microorganisms, along with moderate bactericidal effects on gram-negative (E. coli, P. aeruginosa) and gram-positive (S. Aureus) bacteria. These results highlight the potential of this essential oil for further investigation in the realm of developing novel medicines and herbal preparations. Further clinical studies are needed to assess this potential.

Author Biographies

Vira Odyntsova, Zaporizhzhia State Medical University

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy, Pharmacology and Botany

Olha Denysenko, Zaporizhzhia State Medical University

PhD, Associate Professor

Department of Pharmacognosy, Pharmacology and Botany

Tatiana Shkopynska, Medical College of Zaporizhzhya State Medical and Pharmaceutical University

PhD

Cyclical commission of professional and practical training of department "Pharmacy"

Valentina Mozul, Zaporizhzhia State Medical University

PhD, Associate Professor

Department of Pharmacognosy, Pharmacology and Botany

Nataliia Polishchuk, Zaporizhzhia State Medical University

PhD, Associate Professor

Department of Microbiology, Virology and Immunology

Ilona Aksonova, Запорізький державний медичний університет

PhD, Assistant

Department of Pharmacognosy, Pharmacology and Botany

Volodymyr Holovkin, Zaporizhzhia State Medical University

PhD, Associate Professor

Department of Pharmacognosy, Pharmacology and Botany

Nataliia Zhyvora, National University of Pharmacy

PhD, Associate Professor

Department of Drugs Technology

References

  1. Cairns, T., Young, M., Adams, J., Edberg, B. (Eds.) (2000). Modern roses XI: the world encyclopedia of roses. San Diego: Academic Press, 638.
  2. Boskabady, M. H., Shafei, M. N., Saberi, Z., Amini, S. (2011). Pharmacological Effects of Rosa Damascena. Iranian Journal of Basic Medical Sciences, 14 (4), 295–307.
  3. Griep, S. V. (2021). Heirloom Old Garden Rose Bushes: What Are Old Garden Roses? Gardening. Knowhow. Available at: https://www.gardeningknowhow.com/ornamental/flowers/roses/old-garden-roses.htm
  4. Akram, M., Riaz, M., Munir, N., Akhter, N., Zafar, S., Jabeen, F. et al. (2019). Chemical constituents, experimental and clinical pharmacology of Rosa damascena: a literature review. Journal of Pharmacy and Pharmacology, 72 (2), 161–174. doi: https://doi.org/10.1111/jphp.13185
  5. Nayebi, N., Khalili, N., Kamalinejad, M., Emtiazy, M. (2017). A systematic review of the efficacy and safety of Rosa damascena Mill. with an overview on its phytopharmacological properties. Complementary Therapies in Medicine, 34, 129–140. doi: https://doi.org/10.1016/j.ctim.2017.08.014
  6. Nunes, H. S., Miguel, M. G. (2017). Rosa damascena essential oils: a brief review about chemical composition and biological properties. Trends in Phytochemical Research (TPR), 1 (3), 111–128.
  7. Davoodi, I., Rahimi, R., Abdollahi, M., Farzaei, F., Farzaei, M. H., Memariani, Z., Najafi, F. (2017). Promising effect of Rosa damascena extract on high-fat diet-induced nonalcoholic fatty liver. Journal of Traditional and Complementary Medicine, 7 (4), 508–514. doi: https://doi.org/10.1016/j.jtcme.2017.01.008
  8. Rezvani-Kamran, A., Salehi, I., Shahidi, S., Zarei, M., Moradkhani, S., Komaki, A. (2017). Effects of the hydroalcoholic extract of Rosa damascena on learning and memory in male rats consuming a high-fat diet. Pharmaceutical Biology, 55 (1), 2065–2073. doi: https://doi.org/10.1080/13880209.2017.1362010
  9. Niazi, M., Hashempur, M. H., Taghizadeh, M., Heydari, M., Shariat, A. (2017). Efficacy of topical Rose ( Rosa damascena Mill.) oil for migraine headache: A randomized double-blinded placebo-controlled cross-over trial. Complementary Therapies in Medicine, 34, 35–41. doi: https://doi.org/10.1016/j.ctim.2017.07.009
  10. Hamdamian, S., Nazarpour, S., Simbar, M., Hajian, S., Mojab, F., Talebi, A. (2018). Effects of aromatherapy with Rosa damascena on nulliparous women’s pain and anxiety of labor during first stage of labor. Journal of Integrative Medicine, 16 (2), 120–125. doi: https://doi.org/10.1016/j.joim.2018.02.005
  11. Akin, M., Saki, N. (2019). Antimicrobial, DPPH scavenging and tyrosinase inhibitory activities of Thymus vulgaris, Helichrysum arenarium and Rosa damascena mill. ethanol extracts by using TLC bioautography and chemical screening methods. Journal of Liquid Chromatography & Related Technologies, 42 (7–8), 204–216. doi: https://doi.org/10.1080/10826076.2019.1591977
  12. Mahboubi, M. (2016). Rosa damascena as holy ancient herb with novel applications. Journal of Traditional and Complementary Medicine, 6 (1), 10–16. doi: https://doi.org/10.1016/j.jtcme.2015.09.005
  13. Maruyama, N., Tansho-Nagakawa, S., Miyazaki, C., Shimomura, K., Ono, Y., Abe, S. (2017). Inhibition of Neutrophil Adhesion and Antimicrobial Activity by Diluted Hydrosol Prepared from & Rosa damascene. Biological and Pharmaceutical Bulletin, 40 (2), 161–168. doi: https://doi.org/10.1248/bpb.b16-00644
  14. Lei, Y., Fu, P., Jun, X., Cheng, P. (2018). Pharmacological Properties of Geraniol – A Review. Planta Medica, 85 (1), 48–55. doi: https://doi.org/10.1055/a-0750-6907
  15. Jirovetz, L., Buchbauer, G., Schmidt, E., Stoyanova, A. S., Denkova, Z., Nikolova, R., Geissler, M. (2007). Purity, Antimicrobial Activities and Olfactoric Evaluations of Geraniol/Nerol and Various of Their Derivatives. Journal of Essential Oil Research, 19 (3), 288–291. doi: https://doi.org/10.1080/10412905.2007.9699283
  16. Mączka, W., Wińska, K., Grabarczyk, M. (2020). One Hundred Faces of Geraniol. Molecules, 25 (14), 3303. doi: https://doi.org/10.3390/molecules25143303
  17. Sharma, Y., Rastogi, S. K., Perwez, A., Rizvi, M. A., Manzoor, N. (2019). β-citronellol alters cell surface properties of Candida albicans to influence pathogenicity related traits. Medical Mycology, 58 (1), 93–106. doi: https://doi.org/10.1093/mmy/myz009
  18. Hsouna, A. B., Trigui, M., Mansour, R. B., Jarraya, R. M., Damak, M., Jaoua, S. (2011). Chemical composition, cytotoxicity effect and antimicrobial activity of Ceratonia siliqua essential oil with preservative effects against Listeria inoculated in minced beef meat. International Journal of Food Microbiology, 148 (1), 66–72. doi: https://doi.org/10.1016/j.ijfoodmicro.2011.04.028
  19. Attia, H., Al-Yasi, H., Alamer, K., Ali, E., Hassan, F., Elshazly, S., Hessini, K. (2020). Induced anti-oxidation efficiency and others by salt stress in Rosa damascena Miller. Scientia Horticulturae, 274. doi: https://doi.org/10.1016/j.scienta.2020.109681
  20. Ghavam, M., Afzali, A., Manconi, M., Bacchetta, G., Manca, M. L. (2021). Variability in chemical composition and antimicrobial activity of essential oil of Rosa × damascena Herrm. from mountainous regions of Iran. Chemical and Biological Technologies in Agriculture, 8 (1). doi: https://doi.org/10.1186/s40538-021-00219-6
  21. Panasenko, O. I., Odyntsova, V. M., Denysenko, O. M., Shkopynska, T. Ye., Mozul, V. I., Holovkin, V. V. (2023). Study of the chemical composition of the freon extract of the Damask rose (Rosa damascena Mill.). Current Issues in Pharmacy and Medicine: Science and Practice, 16 (1), 18–22. doi: https://doi.org/10.14739/2409-2932.2023.1.269905
  22. Tanjga, B. B., Lončar, B., Aćimović, M., Kiprovski, B., Šovljanski, O., Tomić, A. et al. (2022). Volatile Profile of Garden Rose (Rosa hybrida) Hydrosol and Evaluation of Its Biological Activity In Vitro. Horticulturae, 8 (10), 895. doi: https://doi.org/10.3390/horticulturae8100895
  23. Badzhelova, V. (2017). In vitro propagation of oil-bearing rose (Rosa damascena Mill.). Agricultural Science and Technology, 9 (3), 194–197. doi: https://doi.org/10.15547/ast.2017.03.035
  24. The State Pharmacopoeia of Ukraine. Vol. 1 (2015). Kharkiv: State Enterprise "Ukrainian Scientific Pharmacopoeial Center for the Quality of Medicinal Products", 1128.
  25. Odyntsova, V. M., Korniievska, V. H., Panchenko, S. V., Korniievskyi, Yu. I., Kokitko, V. I. (2022). Chromato-mass spectrometric study of valerian infusion with various extractants. Current Issues in Pharmacy and Medicine: Science and Practice, 15 (1), 31–39. doi: https://doi.org/10.14739/2409-2932.2022.1.252374
  26. Panasenko, O. I., Mozul, V. I., Denysenko, O. M., Aksonova, I. I., Holovkin, V. V. (2021). Research of the chemical composition of Artemisia tschernieviana Bess. by gas chromatography method with mass detection. Current Issues in Pharmacy and Medicine: Science and Practice, 14 (3), 282–286. doi: https://doi.org/10.14739/2409-2932.2021.3.242650
  27. Aboh, M. I., Yakubu, J. G., U. Eze, J., Khalid-Salako, F., Oladosu, P. O. (2021). Antifungal Potential of Some Nigerian Indigenous Plants: A Remedy for Candidiasis. Journal of Advances in Microbiology, 21 (12), 128–134. doi: https://doi.org/10.9734/jamb/2021/v21i1230421
  28. Steglińska, A., Bekhter, A., Wawrzyniak, P., Kunicka-Styczyńska, A., Jastrząbek, K., Fidler, M. et al. (2022). Antimicrobial Activities of Plant Extracts against Solanum tuberosum L. Phytopathogens. Molecules, 27 (5), 1579. doi: https://doi.org/10.3390/molecules27051579
Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation

Downloads

Published

2023-08-31

How to Cite

Odyntsova, V., Denysenko, O., Shkopynska, T., Mozul, V., Polishchuk, N., Aksonova, I., Holovkin, V., & Zhyvora, N. (2023). Chemical composition and antimicrobial activity of Rosa Damascena mill. (variety rainbow) from clonal micropropagation. ScienceRise: Pharmaceutical Science, (4(44), 89–96. https://doi.org/10.15587/2519-4852.2023.286746

Issue

No. 4(44) (2023)

Section

Pharmaceutical Science

License

Copyright (c) 2023 Vira Odyntsova, Olha Denysenko, Tatiana Shkopynska, Valentina Mozul, Nataliia Polishchuk, Ilona Aksonova, Volodymyr Holovkin, Nataliia Zhyvora

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.