Comparative analysis of essential oil containing raw materials of honeyherb (Lippia dulcis Trevir.) under different growing conditions
DOI:
https://doi.org/10.15587/2519-4852.2023.294908Keywords:
Lippia dulcis Trevir., shoots, essential oil, GC/MS, camphor, germacrene D, caryophyllene, α-bisabolene, quantitative contentAbstract
The use of plant raw materials is one of the areas of modern pharmaceutical science in the production of herbal drugs. In this regard, one of the oldest medicinal plants, Lippia dulcis Trevir (Phyla scaberrima (Juss. ex Pers.) Moldenke) from Verbenaceae family, is of great interest. According to traditional Mexican medicine, Lippia dulcis is used in the treatment of colds, coughs, bronchitis, and digestive disorders as an anti-inflammatory, antitussive, antipyretic, expectorant, emollient and diuretic agent.
The aim. The aim of our study was to identify and determine the quantitative content of essential oils by GC/MS method in Lippia dulcis herb shoots.
Materials and methods. The determination of the essential oil composition of Lippia dulcis was conducted using Agilent Technologies' 6890 chromatograph with mass spectrometric detector 5973 (Agilent Technologies, USA).
Results. The GC/MS method 19 components of essential oil were identified in L. dulcis shoots grown in open soil conditions, the total content of which was 1274.82 μg/g, in L. dulcis shoots grown in closed soil conditions - 23, the total content of which was 2361.11 μg/g. Thus, the total content of essential oil in shoots of L. dulcis grown in closed soil conditions was 1.8 times higher than in shoots of L. dulcis grown in open soil conditions.
Conclusions. The component composition of the essential oil of L. dulcis shoots harvested from plants grown in open soil conditions (LD-1) and closed soil conditions (LD-2) was investigated using the chromato-mass spectrometric method. The following pharmacologically important components - camphor, germacrene D, caryophyllene, α-bisabolene - were found in both samples of the essential oil of L. dulcis shoots in significant quantities, which indicates the prospects for further technological and pharmacological studies of honey herb raw materials
References
- Slobodianiuk, L., Budniak, L., Feshchenko, H., Sverstiuk, A., & Palaniza, Y. (2022). Quantitative analysis of fatty acids and monosaccharides composition in Chamerion angustifolium L. by GC/MS method. Pharmacia, 69 (1), 167–174. doi: https://doi.org/10.3897/pharmacia.69.e76687
- Contreras-Puentes, N., Salas-Moreno, M., Mosquera-Chaverra, L., Córdoba-Tovar, L., Alviz-Amador, A. (2022). Volatile compounds from Phyla scaberrima (Juss. ex Pers.) Moldenke and Dysphania ambrosioides (L.) Mosyakin & Clemants as possible SARS-CoV-2 protease inhibitors: Identification and in-silico study. Journal of Pharmacy & Pharmacognosy Research, 10 (3), 469–485. doi: https://doi.org/10.56499/jppres21.1328_10.3.469
- Morton, J. F. (1981). Atlas of Medicinal Plants of Middle America: Bahamas to Yucatan. C. C. Thomas. Springfield Ill, 746.
- Esteban, N. M. (1982). Plantas medicinales de Puerto Rico: folklore y fundamentos científicos. Editorial de la Unversidad de Puerto Rico, 498.
- Pascual, M. E., Slowing, K., Carretero, E., Sánchez Mata, D., Villar, A. (2001). Lippia: traditional uses, chemistry and pharmacology: a review. Journal of Ethnopharmacology, 76 (3), 201–214. doi: https://doi.org/10.1016/s0378-8741(01)00234-3
- Ombito, J. O., Salano, E. N., Yegon, P. K., Ngetich, W. K., Mwangi, E. M. (2014). A review on the chemistry of some species of genus Lippia (Verbenaceae family). Journal of Scientific and Innovative Research, 3 (4), 460–466. doi: https://doi.org/10.31254/jsir.2014.3411
- Pérez, S., Meckes, M., Pérez, C., Susunaga, A., Zavala, M. A. (2005). Anti-inflammatory activity of Lippia dulcis. Journal of Ethnopharmacology, 102 (1), 1–4. doi: https://doi.org/10.1016/j.jep.2005.06.047
- Rocha, T. T., Araújo, D. X., Assis, R. M. A. de, Carvalho, A. A. de, Bertolucci, S. K. V., Pinto, J. E. B. P. (2023). Establishment and clonal propagation of Lippia dulcis Trevir. through in vitro single node cultures. Plant Cell Culture & Micropropagation, 19. doi: https://doi.org/10.46526/pccm.2023.v19.190
- Ravindran, P. N. (2017). Mexican sweet herb. The encyclopedia of herb and spices, 2, 629–631.
- Attia, M., Kim, S.-U., Ro, D.-K. (2012). Molecular cloning and characterization of (+)-epi-α-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis. Archives of Biochemistry and Biophysics, 527 (1), 37–44. doi: https://doi.org/10.1016/j.abb.2012.07.010
- Petruk, Yu. V. (2022). Syrovynna produktyvnist Phyla scaberrima (Juss. ex Pers.) Moldenke v umovakh vidkrytoho gruntu. Khimiia pryrodnykh spoluk. TNMU, 63–64.
- Petruk, Yu. V. (2022). Pervynne introduktsiine vyprobuvannia Phyla scaberrima (Verbenaceae) yak tsukrozaminnyka ta likarskoi roslyny u Kremenetskomu botanichnomu sadu. Perspektyvni napriamky naukovykh doslidzhen likarskykh ta efirooliinykh kultur. DSLR IAP NAAN – VKF «Inter Park», 31–34.
- Adams, R. P. (2016). Comparison of intensely sweet volatile leaf oils of Lippia dulcis (Verbenaceae) with low and high camphor from Brazil and Mexico. Phytologia, 98 (3), 207–215.
- Budniak, L., Slobodianiuk, L., Marchyshyn, S., Ilashchuk, P. (2021). Determination of polysaccharides in Gentiana cruciata L. herb. Pharmacologyonline, 2, 1473–1479.
- Budniak, L., Slobodianiuk, L., Kravchuk, L., Kalynyuk, T. (2021). Investigation of antibacterial and antifungal activities of the herb of Tropaeolum majus L. Pharmacologyonline, 3, 937–947.
- Budniak L, Slobodianiuk L, Marchyshyn S, Parashchuk E (2021d) Determination of carbohydrates in burnet saxifrage (Pimpinella saxifraga L.). Pharmacologyonline, 2, 1374-1382.
- Derzhavna Farmakopeia Ukrainy. T. 1. (2015). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
- Souto-Bachiller, F. A., De Jesus-Echevarría, M., Cárdenas-González, O. E., Acuña-Rodriguez, M. F., Meléndez, P. A., Romero-Ramsey, L. (1997). Terpenoid composition of Lippia dulcis. Phytochemistry, 44 (6), 1077–1086. doi: https://doi.org/10.1016/s0031-9422(96)00691-7
- Raal, A., Orav, A., Arak, E. (2007). Composition of the essential oil ofSalvia officinalisL. from various European countries. Natural Product Research, 21 (5), 406–411. doi: https://doi.org/10.1080/14786410500528478
- Derzhavna Farmakopeia Ukrainy (2018). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 416.
- Bondarenko, V. H., Kanivska, I. Yu., Paramonova, S. M. (2006). Teoriia ymovirnostei i matematychna statystyka. Kyiv: NTUU «KPI», 125.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Svitlana Marchyshyn, Liudmyla Slobodianiuk, Liliia Budniak, Iryna Dakhym, Larysa Boyko, Mariia Kyryliv, Iryna Bekus
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
