Investigation of the influence of the extract of the herb of umbellate wintergreen on the structural and functional state of the kidneys of rats

Abstract

Experimental studies were carried out as part of a preclinical study of the umbellate wintergreen herb extract regarding the possibility of using the phytoremediсal agent in diseases of the kidneys and urinary tract.

The aim. Study of the effect of umbellate wintergreen herb extract on the structural and functional state of kidneys in rats.

Materials and methods. The kidneys of rats were studied after: a single subcutaneous injection of 2.5 % potassium chromate solution at a dose of 0.7 ml/kg on the first day of the experiment - control pathology; administration of an alcoholic extract of the umbellate wintergreen herb (UWH) at a dose of 3 ml/kg or a reference preparation of a solution of lespefril at a dose of 3 ml/kg starting from the second day of the experiment (after the induction of renal failure) daily for 20 days in the form of aqueous solutions prepared on a physiological solution; intact rats that received an equivalent amount of physiological solution.

The following morphological indicators were used to assess the condition of the renal corpuscles: degree of expansion of the Shumlyansky-Bowman capsule, mesangium; segmental intracapillary proliferation. The degree of expressiveness of atrophy, edema, vacuolar dystrophy and disorganization of the nephrothelium, tubulohidrosis was evaluated.

Results. After treatment with umbellate wintergreen extract in rats with chromate-induced renal failure, more preservation of the cytoarchitectonics of the renal tissue was observed. In most of the renal corpuscles, the size of the urinary space is clearly reduced. In the vascular glomeruli, as a rule, the pattern of the capillary network is clear, the thickening of the BM of the capillaries is not observed. The saturation of mesangial and endothelial cells in most glomeruli is close to the intact control. In general, the expressiveness of changes in the glomerular apparatus in rats after administration of UWH decreased by 44.87 % compared to rats with renal failure that were not treated with the extract.

Conclusions. The extract of the umbellate wintergreen herb helps to protect the morphostructure of the kidney tissue of rats with chromate-induced renal failure, reducing the degree of damage to the renal corpuscles and tubules. In terms of the expressiveness of its nephroprotective properties, the extract of umbellate wintergreen was inferior to the reference drug Lespefril solution in its effect on the state of the glomerular apparatus and ahead of it in its effect on the state of the tubular system.

After treatment with the extract in rats with chromate-induced renal failure, more preservation of the cytoarchitectonics of the renal tissue was noted. In most of the renal corpuscles, the size of the urinary space is clearly reduced. In the vascular glomeruli, as a rule, the pattern of the capillary network is clear, the thickening of the BM of the capillaries is not observed. The saturation of mesangial and endothelial cells in most glomeruli is close to the intact control