Influence of gels containing glucosamine and silver nanoparticles on the peroxidation process under burn wounds conditions in rats
DOI:
https://doi.org/10.15587/2519-4852.2016.72814Keywords:
wounds, burns, gel, Silver nanoparticles, Glucosamine hydrochloride, lipids and proteins peroxidationAbstract
Burns ranks third in the overall structure of injuries. For efficient pharmacocorrection of burns, local medicines must show a complex effect on pathogenic process. Oxidative stress prevention in wound eliminates secondary necrosis development and creates favorable conditions for injured tissues regeneration.
Aim of the given research was to study the influence of gels containing Glucosamine and Silver nanoparticles on the proteins and lipids peroxidation processes under burn wounds conditions in rats.
Materials and methods. The second degree thermal burn injury was reproduced using special device with a metal plate with a diameter of 2.5 cm at the end. The exposure time of the contact plate heated to 200 oC was 4 sec. The gels were applied to the affected areas daily in the experimentally determined dose of 50 mg/cm2. Blood sampling for analysis was carried out in two periods: the 7th day of treatment, when the scabs began to peel, and the 15th day of treatment. Each 6 rats from every group were decapitated, and proteins oxidative level (aldehyde and ketone dinitrophenylhydrazones), lipids oxidative level (the content of diene conjugates and TBA-r), free SH-groups were determined.
Results. During the animal burns treatment by gel containing Glucosamine and gel containing Silver nanoparticles and Glucosamine, a reliable decrease in the intensity of proteins oxidative modification processes of 60 % and lipids peroxidation processes of 58 % happened. Normalization of organism antioxidative system was determined, which reduces oxidative stress effects and promotes injured tissues regeneration processes.
Conclusion. Pharmacological study of Silver nanoparticles and Glucosamine is promising for local wound-healing remedies of new generation creation and improvement of efficiency of wounds pharmacocorrection
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