Development of the method for qualitative analysis of shepherd’s purse herb to be included into the state pharmacopoea of ukraine draft national monograph

Authors

  • Вікторія Юріївна Кузнєцова National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-4146-689X
  • Вікторія Сергіївна Кисличенко National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002, Ukraine
  • Еліна Едуардівна Котова Ukrainian scientific Pharmacopoeial center for quality of medicines Astronomichna str., 33, Kharkiv, Ukraine, 61085, Ukraine
  • Андрій Георгієвич Котов Ukrainian scientific Pharmacopoeial center for quality of medicines Astronomichna str., 33, Kharkiv, Ukraine, 61085, Ukraine
  • Наталя Анатоліївна Сущук Odessa National Medical University Valikhovskiy lane, 2, Odessa, Ukraine, 65082, Ukraine

DOI:

https://doi.org/10.15587/2519-4852.2016.86172

Keywords:

the State Pharmacopoeia of Ukraine, Shepherd’s purse herb, flavonoids, hydroxycinnamic acids, amino acids

Abstract

Shepherd’s purse herb is described in the State Pharmacopoeia of the USSR XI edition, the British Pharmacopoeia (BHP, 1996 «Shepherd´s Purse»), French Pharmacopoeia (Pharmacopee Francaise, 1998 «Bourse A Pasteur»), and German Pharmaceutical Codex (DAC Monograph 2004 «Hirtentäschelkraut»). Previously, it was reported that the modern methods for identification of biologically active compounds of Shepherd’s purse herb were absent in the State Pharmacopoeia of the USSR XI edition, but identification was carried out by external and microscopic diagnostic features.

Aim. Development of methods, harmonized with the SPhU requirements to herbal material, for identification of Shepherd’s purse herb using TLC method, for implementation to the Draft national Monograph “Shepherd’s purse herb”.

Methods. To achieve the mentioned goal, standardized methods of analysis of phenol compounds and amino acids by TLC method, described in the State Pharmacopoeia of Ukraine monographs, were used.

Results. Concerning chemical composition, medicinal use, and approaches to standardization, the following classes of compounds were selected for TLC identification: flavonoids, hydroxycinnamic acids, and amino acids. Development of the methods was carried out together with their validation according to the SPhU general article requirements.

Conclusion. Premise for “Identification” partition development for implementation to the SPhU Monograph “Shepherd’s purse herb” was substantiated. TLC methods for identification of flavonoids together with both hydroxycinnamic acids and amino acids, were developed. The analysis of different samples of Shepherd’s purse herb was carried out under the developed methods conditions. The possibility of including of “Identification C” flavonoids identification and “Identification D” amino acids identification into the Draft national Monograph “Shepherd’s purse herb” using TLC by the developed methods was confirmed

Author Biographies

Вікторія Юріївна Кузнєцова, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Chemistry of Natural Compounds

Вікторія Сергіївна Кисличенко, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of pharmaceutical science, professor, Head of the Department

Department of Chemistry of Natural Compounds

Еліна Едуардівна Котова, Ukrainian scientific Pharmacopoeial center for quality of medicines Astronomichna str., 33, Kharkiv, Ukraine, 61085

PhD, Senior Researcher, Head of Sector

Sector of development of monographs on herbal drugs

Андрій Георгієвич Котов, Ukrainian scientific Pharmacopoeial center for quality of medicines Astronomichna str., 33, Kharkiv, Ukraine, 61085

Doctor of pharmaceutical science, Senior Researcher, Head of department

Department of State Pharmacopoeia of Ukraine

Наталя Анатоліївна Сущук, Odessa National Medical University Valikhovskiy lane, 2, Odessa, Ukraine, 65082

PhD, Assistant

Department of Pharmacognosy and Drug Technology

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Published

2016-12-28

How to Cite

Кузнєцова, В. Ю., Кисличенко, В. С., Котова, Е. Е., Котов, А. Г., & Сущук, Н. А. (2016). Development of the method for qualitative analysis of shepherd’s purse herb to be included into the state pharmacopoea of ukraine draft national monograph. ScienceRise: Pharmaceutical Science, (4 (4), 24–29. https://doi.org/10.15587/2519-4852.2016.86172

Issue

No. 4 (4) (2016)

Section

Pharmaceutical Science

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Copyright (c) 2016 Вікторія Юріївна Кузнєцова, Вікторія Сергіївна Кисличенко, Еліна Едуардівна Котова, Андрій Георгієвич Котов, Наталя Анатоліївна Сущук

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