Chromatography-mass spectrometry study of low molecular aliphatic, fatty and aromatic acids of Veronica teucrium L. rhizomes

Authors

DOI:

https://doi.org/10.15587/2519-4852.2017.99317

Keywords:

Veronica teucrium L., aliphatic acids, fatty acids, aromatic acids, gas chromatography/mass spectrometric method

Abstract

The aim of our research was to identify and quantify of aliphatic, aromatic and fatty acids of Veronica teucrium L. rhizomes by the gas chromatography/mass spectrometric method.

Methods. The object of study was V. teucrium L. rhizomes, collected in 2015 in Kharkiv region. The analysis of acid’s methyl esters was performed using chromatography-mass spectrometer 5973N/6890N MSD/DS Agilent Technologies.

The injection of sample in chromatographic capillary column INNOWAX (0.25 mm х 30 m) was performed by a splitless mode. The identification of acid’s methyl esters were performed by calculating of the equivalent length of the aliphatic chain (ECL); using data from the mass spectra libraries NIST 05 and Willey 2007 in conjunction with programs for identifying – AMDIS and NIST; also retention time of esters was compared with the retention time of standard compounds (Sigma). Internal standard method was used for quantitative calculations.

Results of the Research. By means of gas chromatography/mass spectrometric method the reseach of carboxylic acids of V. teucrium L. rhizomes have been studied for first time, and in the result of the study 10 aliphatic, 16 fatty and 9 aromatic acids had been identified and quantified. The total content of identified carboxylic acids of V. teucrium L. rhizomes was 3068.06 mg/% (3.07%). Among low molecular aliphatic acids malic, citric and levulinic; among fatty acids – saturated: myristic, palmitic and tetracosanoic, and unsaturated: oleic, linoleic and linolenic are dominant. The particular importance have identified aromatic acids – benzoic; phenolcarbonic: vanillic, veratrylic, р-hydroxybenzoic, gentisic, syringic and hydroxycinnamic: р-coumaric.

Conclusions. In the first time in V. teucrium L. rhizomes 10 low molecular aliphatic acids, 16 fatty acids and 9 aromatic acids had been identified and quantified by using gas chromatography/mass spectrometric method

Author Biographies

Alina Osmachko, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Postgraduate student

Department of Pharmacognosy 

Alla Kovaleva, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmacy, Professor

Department of Pharmacognosy

Ilyina Tatyana, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmacy, Associate professor, Professor

Department of Pharmacognosy 

Oleg Koshovyi, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmacy, Associate professor, Head of Department

Department of Pharmacognosy 

Natali Sidora, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate professor

Department of Pharmacognosy 

References

  1. Albach, D. C., Grayer, R. J., Jensen, S. R., Ozgokce, F., Veitch, N. C. (2003). Acylated flavone glycosides from Veronica. Phytochemistry, 64 (7), 1295–1301. doi: 10.1016/j.phytochem.2003.08.012
  2. Belenovskaya, L., Korhov, V., Mac, M., Medvedeva, L. (Eds.) (1990). Rastitelnyye resursy SSSR. Tsvetkovyye rasteniya, ikh khimicheskiy sostav, ispolzovaniye. Semeystvo Caprifoliaceae – Plantaginaceae. Leningrad: Nauka, 328.
  3. Mocan, A., Vodnar, D., Vlase, L., Crisan, O., Gheldiu, A.-M., Crisan, G. (2015). Phytochemical characterization of Veronica officinalis L., V. teucrium L. and V. orchidea Crantz from Romania and their antioxidant and antimicrobial properties. International Journal of Molecular Sciences, 16 (9), 21109–21127. doi: 10.3390/ijms160921109
  4. Chekhirova, G. V., Aseyeva, Т. А., Каshin, V. K. (2012). Rasteniya semeystva Scrophulariaceae v tibetskoy meditsine. Vestnik buryatskogo gosudarstvennogo universiteta, S/2012, 181–184.
  5. Gusev, N. F., Nemereshina, О. N. (2012). Poisk vitaminosnykh rasteniy v stepnoy i lesostepnoy zonakh orenburgskogo preduralya. Nauka Krasnodarya, 1, 19–27.
  6. Jensen, S. R., Albach, D. C., Ohno, T., Grayer, R. J. (2005). Veronica: Iridoids and cornoside as chemosystematic markers. Biochemical Systematics and Ecology, 33 (10), 1031–1047. doi: 10.1016/j.bse.2005.03.001
  7. Zivkovic, J., Cebovic, T., Maksimovic, Z. (2012). In vivo and in vitro antioxidant effects of three Veronica spesies. Open Life Sciences, 7 (3), 559–568. doi: 10.2478/s11535-012-0041-4
  8. Beara, I., Zivkovic, J., Lesjak, M., Ristic, J., Savikin, K., Maksimovic, Z., Jankovic, T. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. Industrial Crops and Products, 63, 276–280. doi: 10.1016/j.indcrop.2014.09.034
  9. Bubenchikova, V. N., Kondratova, Yu. A. (2010). Rasteniya roda Veronika. Kursk: GOU VPO KGMUroszdrava, 104.
  10. Crisan, G., Vlase, L., Balica, G., Muntean, D., Stefanescu, C., Paltinean, R. et. al. (2010). LC/MC analysis of aukubin and catalpol of some Veronica species. Farmacia, 58 (2), 237–242.
  11. Osmachko, A. P., Kovaleva, A. M. (2016). Pharmacognostic study of Veronica teucrium L. herb. 2nd International Young Scientists Symposium «Plants in Pharmacy & Nutrition». Wroclaw: Wroclaw Medical University, 132.
  12. Osmachko, А. P., Kovaleva, А. М., Iliina, Т. V., Koshovii, О. N. (2015). Doslidzhennya fenolnikh rechovin travi Veronica teucrium L. Zbirnik naukovikh prats spivrobitnikiv NMAPO іm. P. L. Shupika, 24 (5), 118–123.
  13. Nour, V., Trandafir, I., Cosmulescu, S. (2012). HPLC Determination of Phenolic Acids, Flavonoids and Juglone in Walnut Leaves. Journal of Chromatographic Science, 51 (9), 883–890. doi: 10.1093/chromsci/bms180
  14. Ghasemzadeh, A., Ghasemzadeh, N. (2011). Flavonoids and phenolic acids: Role and biochemicalactivity in plants and human. Journal of Medicinal Plants Research, 5 (31), 6697–6703. doi: 10.5897/jmpr11.1404

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Published

2017-04-29

How to Cite

Osmachko, A., Kovaleva, A., Tatyana, I., Koshovyi, O., & Sidora, N. (2017). Chromatography-mass spectrometry study of low molecular aliphatic, fatty and aromatic acids of Veronica teucrium L. rhizomes. ScienceRise: Pharmaceutical Science, (2 (6), 22–25. https://doi.org/10.15587/2519-4852.2017.99317

Issue

No. 2 (6) (2017)

Section

Pharmaceutical Science

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Copyright (c) 2017 Alina Osmachko, Alla Kovaleva, Ilyina Tatyana, Oleg Koshovyi, Natali Sidora

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