Justification of the preliminary enzymatic treatment of berries of dogwood, black elderberry, hawthorn, barberry and raspberry, grown in the territory of the Republic of Azerbaijan
DOI:
https://doi.org/10.15587/2706-5448.2026.352898Keywords:
berries, enzyme preparations, multi-enzyme composition, juice yield, chemical composition, carbohydrates, pectinAbstract
The research shows the results of the effect of preliminary enzymatic treatment of dogwood berries, black elderberry, hawthorn, barberry and raspberry on juice yield and extraction of valuable chemical compounds and nutrients into juice. The objects of the research were the technology for enzymatic extraction of dogwood berries, black elderberries, hawthorn berries, barberries, and raspberries. Enzyme preparations with pectolytic and gluconolytic activity were also investigated. The enzymes included Pectinex BE XXL, Pectinex Yieldash Extra, Amylase AG 300 L, and Fructozym P. Additional preparations used in the research were Rapidaza CR, Bryuzaym BGX, Laminex Super, Selloviridin Q20X, Sellokyuks-A Extra, and Enzerzim XT. The experiment used modern photometric, thermogravimetric, refractometric, potentiometric, chromatographic and other methods of analysis. It was established that the use of a composition of enzyme preparations with pectolytic and glucanolytic action Pectinex BE XXL, Amylase AG 300 L, Rapidaza CR, Sellokyuks-A contributes to an increase in the yield of unclarified juices by an average of 6.8–9.4% compared to the control samples. In this case, the most effective are the use of Fructosym P, Pectinex BE XXL and Amylase AG300L, and when using multienzyme complexes, MFK-I and MFK-II turned out to be more effective, each consisting of a mixture of two enzymes, the juice yield increased by an average of 11.0–14.4% or 1.19–1.29 times compared to the control. The use of the enzyme preparation composition allows to increase the yield of valuable natural components and functional food ingredients in the juice – total sugars by 1.05–1.23 times, organic acids by 1.04–1.24 times, pectin substances by 1.06–1.31 times, ash substances by 1.19–1.33 times, vitamin C by 1.18–1.38 times. The extraction of natural antioxidants and preservatives increases the nutritional value of the juice and improves its technological properties.
References
- Magerramov, M. A. (2020). Nauchnye osnovy proizvodstva, teplo i elektrofizicheskie svoistva plodoovoshchnykh sokov. Liankaran: LGU, 321.
- Rajendran, P., Nandakumar, N., Rengarajan, T., Palaniswami, R., Gnanadhas, E. N., Lakshminarasaiah, U. et al. (2014). Antioxidants and human diseases. Clinica Chimica Acta, 436, 332–347. https://doi.org/10.1016/j.cca.2014.06.004
- Loi, M., Paciolla, C. (2021). Plant Antioxidants for Food Safety and Quality: Exploring New Trends of Research. Antioxidants, 10 (6), 972. https://doi.org/10.3390/antiox10060972
- Olas, B. (2018). Berry Phenolic Antioxidants – Implications for Human Health? Frontiers in Pharmacology, 9. https://doi.org/10.3389/fphar.2018.00078
- Lourenço, S. C., Moldão-Martins, M., Alves, V. D. (2019). Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules, 24 (22), 4132. https://doi.org/10.3390/molecules24224132
- Novruzov, E. N., Mustafaeva, L. A., Zeynalli, A. Z. (2019). Comparative study of the chemical composition and antioxidant properties of extracts of various cornel (Cornus mas L.) organs. Plant & Fungal Research, 2 (2), 41–48. https://doi.org/10.29228/plantfungalres.23
- Məhərrəmov, M. Ə., Məhərrəmova, S. İ., Kazımova, İ. H. (2019). Xammal və qida məhsullarının təhlükəsizliyi. Bakı: “İqtisad Universiteti” Nəşriyyatı, 270. Available at: https://mek.org.az/e-kitabxana/kitab.php?id=22902
- Sharma, H. P., Patel, H., Sugandha. (2016). Enzymatic added extraction and clarification of fruit juices – A review. Critical Reviews in Food Science and Nutrition, 57 (6), 1215–1227. https://doi.org/10.1080/10408398.2014.977434
- Məhərrəmov, M. Ə. (2017). Qida məhsulları texnologiyasının nəzəri əsasları. Bakı: “İqtisad Universiteti” Nəşriyyatı, 384. Available at: https://www.preslib.az/az/book/vyhYX3HmJrLlGIk
- Aït-Kaddour, A., Hassoun, A., Tarchi, I., Loudiyi, M., Boukria, O., Cahyana, Y. et al. (2024). Transforming plant-based waste and by-products into valuable products using various “Food Industry 4.0” enabling technologies: A literature review. Science of The Total Environment, 955, 176872. https://doi.org/10.1016/j.scitotenv.2024.176872
- Koponen, J. M., Buchert, J., Poutanen, K. S., Törrönen, A. R. (2007). Effect of pectinolytic juice production on the extractability and fate of bilberry and black currant anthocyanins. European Food Research and Technology, 227 (2), 485–494. https://doi.org/10.1007/s00217-007-0745-2
- Liu, S., Chang, X., Liu, X., Shen, Z. (2016). Effects of pretreatments on anthocyanin composition, phenolics contents and antioxidant capacities during fermentation of hawthorn (Crataegus pinnatifida) drink. Food Chemistry, 212, 87–95. https://doi.org/10.1016/j.foodchem.2016.05.146
- Marsol-Vall, A., Kelanne, N., Nuutinen, A., Yang, B., Laaksonen, O. (2021). Influence of enzymatic treatment on the chemical composition of lingonberry (Vaccinium vitis-idaea) juice. Food Chemistry, 339, 128052. https://doi.org/10.1016/j.foodchem.2020.128052
- Kruszewski, B., Boselli, E. (2024). Blackcurrant Pomace as a Rich Source of Anthocyanins: Ultrasound-Assisted Extraction under Different Parameters. Applied Sciences, 14 (2), 821. https://doi.org/10.3390/app14020821
- Bamba, B. S. B., Shi, J., Tranchant, C. C., Xue, S. J., Forney, C. F., Lim, L.-T. (2018). Influence of Extraction Conditions on Ultrasound-Assisted Recovery of Bioactive Phenolics from Blueberry Pomace and Their Antioxidant Activity. Molecules, 23 (7), 1685. https://doi.org/10.3390/molecules23071685
- Li, X., Zhu, F., Zeng, Z. (2021). Effects of different extraction methods on antioxidant properties of blueberry anthocyanins. Open Chemistry, 19 (1), 138–148. https://doi.org/10.1515/chem-2020-0052
- Maharramova, S. (2023). Changes in the chemical composition of extracts of wild berries growing in the Republic of Azerbaijan during enzimatic pretreatment of their pulp. Ukrainian Food Journal, 12, 542–555. https://doi.org/10.24263/2304-974x-2023-12-4-5
- Magerramova, S. I. (2022). Vliianie predvaritelnoi fermentativnoi obrabotki na biologicheski aktivnye soedienie i antioksidantnye svoistva nekotorykh iagod. Technical and Agrarian sciences, 1, 46–51. Available at: https://lsu.edu.az/new/imgg/aqrar-2023/tex_aqr_1_2022.pdf
- Qədimova, N. S. (2025). Bitki mənşəli bioloji fəal əlavələrin tətbiqi ilə içkilərin texnologıyasının təkmilləşdirilməsi. [Extended abstract of Doctoral dissertation]. Available at: https://adau.edu.az/images/files/2025-05/1748261455_avtoreferat-az-qdimova-natvan-a5-3.pdf
- Qasımov, H. (2017). Naxçıvan Muxtar Respublikası florasının alkoqollu içki və sirkə alınan bitkiləri. Elmi Əsərlər, 3 (84), 33–40. Available at: https://ndu.edu.az/wp-content/uploads/Elmi%20Eserler/84%20tebiet%202017.pdf
- İbrahimov, Ə., İbrahimov, Ə., Qurbanov, Ə. (2017). Naxçıvan Muxtar Respublikasının bəzi perspektivli yabanı qida meyvə bitkilərinin xalq təsərrüfatı əhəmiyyəti. Elmi Əsərlər, 7, 33–39. Available at: https://ndu.edu.az/wp-content/uploads/Elmi%20Eserler/88%20t%E2%95%94%D0%A9bi%E2%95%94%D0%A9t%202017.pdf
- Ionsecu, A.-D., Voicu, G., Ferdes, M., Ipate, G., Constantin, G.-A., Stefan, E.-M., Begea, M. (2024). Effect of Grinding and Successive Sieving on Active Biological Compounds in the Obtained Fractions of Blackthorn Berries. https://doi.org/10.20944/preprints202406.1934.v1
- Bastos, K. V. L. da S., de Souza, A. B., Tomé, A. C., Souza, F. de M. (2025). New Strategies for the Extraction of Antioxidants from Fruits and Their By-Products: A Systematic Review. Plants, 14 (5), 755. https://doi.org/10.3390/plants14050755
- Nicolescu, A., Babotă, M., Zhang, L., Bunea, C. I., Gavrilaș, L., Vodnar, D. C. et al. (2022). Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from Rosa canina L. Pseudo-Fruits (Rosehip) and Their Biological Activity. Antioxidants, 11 (6), 1123. https://doi.org/10.3390/antiox11061123
- Al-Khayri, J. M., Rashmi, R., Toppo, V., Chole, P. B., Banadka, A., Sudheer, W. N. et al. (2023). Plant Secondary Metabolites: The Weapons for Biotic Stress Management. Metabolites, 13 (6), 716. https://doi.org/10.3390/metabo13060716
- Cavdar Dincturk, H., Can Karaca, A., Capanoglu, E. (2025). Development of novel processing technologies for sustainable production of berries. Berry Fruits, 325–341. https://doi.org/10.1016/b978-0-443-16011-0.00009-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 Sevinj Maharramova, Ilhama Kazimova, Mikail Maharramov

This work is licensed under a Creative Commons Attribution 4.0 International License.
The consolidation and conditions for the transfer of copyright (identification of authorship) is carried out in the License Agreement. In particular, the authors reserve the right to the authorship of their manuscript and transfer the first publication of this work to the journal under the terms of the Creative Commons CC BY license. At the same time, they have the right to conclude on their own additional agreements concerning the non-exclusive distribution of the work in the form in which it was published by this journal, but provided that the link to the first publication of the article in this journal is preserved.



