Experimental research on development and validation of methods of quantitative determination of flavonoids and essential oil in solid multi-component capsules "Uroholum"

Authors

  • Oleksandr Shmalko National University of Pharmacy Pushkivska str., 53, Kharkiv, Ukraine, 61002, Ukraine
  • Nataliia Bevz National University of Pharmacy Pushkivska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-7259-8908
  • Igor Vyshnevskyy Farmatsevtycheskaya Fabryka, Ooo Dkp Koroleva str., 4, Stanishivka, Zhytomyr Region, 12430, Ukraine
  • Liliia Vyshnevska National University of Pharmacy, Pushkivska str., 53, Kharkiv, Ukraine, 61002, Ukraine https://orcid.org/0000-0002-6887-3591

DOI:

https://doi.org/10.15587/2519-4852.2019.190552

Keywords:

standardization, multicomponent composition, dry extract, spectrophotometry, validation, solid capsules

Abstract

The aim. Development and validation of methods for the quantification of flavonoids and essential oils in solid multicomponent capsules for use in urology.

Methods. To quantify the amount of flavonoids, a spectrophotometric technique was developed and validated using an Evolution 60 s spectrophotometer from Thermo Fisher Scientific, USA, to determine the essential oil - gravimetric method.

Results. As a result of the study, a method was developed to quantify the sum of substances of the flavonoid structure of the total phytoextract in the form of capsules by spectrophotometric method and the sum of essential oils after pre-distillation with water vapour and extraction with organic solvent by gravimetry. Spectrophotometric quantification of flavonoid structure substances was performed after complexation reaction with aluminum salts (III) in acetic acid medium at an analytical wavelength of 410 nm. The calculation of the content of biologically active substances was performed by the standard method, which was used as a rutin. Validation parameters such as specificity, linearity, and precision have been studied for methods for quantifying the sum of substances of flavonoid structure and essential oils.

Conclusions. Available and validated methods for the quantitative determination of the amount of biologically active substances of a dry multicomponent herbal extract in solid capsules have been developed and validated. The studied validation parameters meet the eligibility criteria

Author Biographies

Oleksandr Shmalko, National University of Pharmacy Pushkivska str., 53, Kharkiv, Ukraine, 61002

Department of drug technology

Nataliia Bevz, National University of Pharmacy Pushkivska str., 53, Kharkiv, Ukraine, 61002

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Igor Vyshnevskyy, Farmatsevtycheskaya Fabryka, Ooo Dkp Koroleva str., 4, Stanishivka, Zhytomyr Region, 12430

PhD, general director

Liliia Vyshnevska, National University of Pharmacy, Pushkivska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of drug technology

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Published

2019-12-30

How to Cite

Shmalko, O., Bevz, N., Vyshnevskyy, I., & Vyshnevska, L. (2019). Experimental research on development and validation of methods of quantitative determination of flavonoids and essential oil in solid multi-component capsules "Uroholum". ScienceRise: Pharmaceutical Science, (6 (22), 43–49. https://doi.org/10.15587/2519-4852.2019.190552

Issue

No. 6 (22) (2019)

Section

Pharmaceutical Science

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Copyright (c) 2019 Oleksandr Shmalko, Nataliia Bevz, Igor Vyshnevskyy, Liliia Vyshnevska

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