Wound healing effect of “Prolidoxid” and “Dexpanthenol with ceramides”: a сomparative study based on the model of chemical burns with expressed alterative skin processes

Authors

DOI:

https://doi.org/10.15587/2519-4852.2021.230031

Keywords:

chemical wounds, dexpanthenol, ceramides, propolis, lidocaine, wound-healing effect, anti-inflammatory effect, healing area, cream, ointment

Abstract

Pharmacotherapy of chemical skin injuries remains an urgent issue today due to its serious health consequences and possible development of complications. Topical treatment is one of the most effective methods of wound treatment using hydrophilic ointments and creams. For now, range of hydrophilic medicinal products with a broad spectrum of action is limited. That is why a search and development of new hydrophilic ointments and creams remains an urgent challenge.

The aim. The aim of this research was to study effectiveness of ointment “Prolidoxid” and cream “Dexpanthenol with ceramides” on the experimental chemical burn model in rats.

Materials and methods. Wound healing effect of ointment “Prolidoxid” and cream “Dexpanthenol with ceramides” was proved by the study of planimetric and hematological parameters on the model of acetic acid burns in rats.

Results. Using animal model of chemical burns it was found that ointment “Prolidoxid” and cream “Dexpanthenol with ceramides” accelerate wound healing on Day 5 and Day 6, consequently, compared to untreated control animals, but in comparison with the action of reference medicine wounds were healed two days faster. Hematological parameters showed that studied medicines inhibit inflammation and reactivate blood rheological properties.

Conclusions. The results suggest that effectiveness of cream “Dexpanthenol with ceramides” is higher than for one-component cream “Dexpanthenol”, but ointment “Prolidoxid” exceeds therapeutic action of ointment “Wundahyl” as reported by hematological and planimetric parameters

Author Biographies

Yaroslava Butko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacology and Pharmacotherapy

Oksana Tkachova, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmaceutical Management and Marketing

Tetyana Tishakova, Kharkiv National Medical University

PhD, Associated Professor

Department of Medical and Bioorganic Chemistry

Anatoly Gordienko, National Technical University “Kharkiv Polytechnic Institute”

Doctor of Pharmaceutical Sciences, Professor

Department of Organic Synthesis and Nanotechnology

Yevhen Bondariev, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Associate Professor

Department of Clinical Pharmacology

Institute for Advanced Training of Pharmacy Specialists

References

  1. Koehler, J., Brandl, F. P., Goepferich, A. M. (2018). Hydrogel wound dressings for bioactive treatment of acute and chronic wounds. European Polymer Journal, 100, 1–11. doi: http://doi.org/10.1016/j.eurpolymj.2017.12.046
  2. Dong, Y., Cui, M., Qu, J., Wang, X., Kwon, S. H., Barrera, J. et. al. (2020). Conformable hyaluronic acid hydrogel delivers adipose-derived stem cells and promotes regeneration of burn injury. Acta Biomaterialia, 108, 56–66. doi: http://doi.org/10.1016/j.actbio.2020.03.040
  3. Jeschke, M. G., Gauglitz, G. G.; Jeschke, M. G., Kamolz, L.-P., Sjöberg, F., Wolf, S. E. (Eds.) (2020). Pathophysiology of Burn Injuries. Handbook of Burns. Vol. 1. Berlin/Heidelberg Springer, 229–245. doi: http://doi.org/10.1007/978-3-030-18940-2_18
  4. Kaddoura, I., Abu-Sittah, G., Ibrahim, A., Karamanoukian, R., Papazian, N. (2017). Burn injury: Review of pathophysiology and therapeutic modalities in major burns. Annals of Burns and Fire Disasters, 30, 95–102.
  5. Brassolatti, P., de Andrade, A. L. M., Bossini, P. S., Otterço, A. N., Parizotto, N. A. (2018). Evaluation of the low-level laser therapy application parameters for skin burn treatment in experimental model: A systematic review. Lasers in Medical Science, 33 (5), 1159–1169. doi: http://doi.org/10.1007/s10103-018-2526-5
  6. Stoica, A. E., Chircov, C., Grumezescu, A. M. (2020). Hydrogel dressings for the treatment of burn wounds: an up-to-date overview. Materials, 13 (12), 2853. doi: http://doi.org/10.3390/ma13122853
  7. Gnaneswaran, N., Perera, E., Perera, M., Sawhney, R. (2015). Cutaneous chemical burns: assessment and early management. Australian Family Physician, 44 (3), 135–139.
  8. Friedstat, J., Brown, D., Levi, B. (2017). Chemical, electrical, and radiation injuries. Clinics in Plastic Surgery, 44 (3), 657–669. doi: http://doi.org/10.1016/j.cps.2017.02.021
  9. Palao, R., Monge, I., Ruiz, M., Barret, J. (2010). Chemical burns: pathophysiology and treatment. Burns, 36 (3), 295–304. doi: http://doi.org/10.1016/j.burns.2009.07.009
  10. Nguyen, A. T. M., Chamberlain, K., Holland, A. J. A. (2021). Paediatric chemical burns: a clinical review. European Journal of Pediatrics, 180 (5), 1359–1369. doi: http://doi.org/10.1007/s00431-020-03905-z
  11. Gürbüz, K., Demir, M., Eroğlu, O., Başaran, A., Yarçe, A., Özlü, Ö. (2020). Demographic and clinical profile of pediatric cutaneous-chemical and contact burn cases: a seven-year retrospective analysis of a burn center. Şehir Tıp Dergisi, 1 (2), 52–57. doi: http://doi.org/10.37609/cmj.1497
  12. Best practice guidelines: effective skin and wound management of non-complex burns (2014). Wounds International. Available at: https://www.woundsinternational.com/resources/details/best-practice-guidelines-effective-skin-and-wound-management-in-non-complex-burns
  13. Guillermo, G. R. F., Hernández, G. J., Pech, P. M. (2018). Topical composition a new alternative in the treatment of burns. Nursing & Care Open Access Journal, 5 (1), 29–32. doi: http://doi.org/10.15406/ncoaj.2018.05.00114
  14. Tarameshloo, M., Norouzian, M., Zarein-Dolab, S., Dadpay, M., Mohsenifar, J., Gazor R. (2012). Aloe vera gel and thyroid hormone cream may improve wound healing in Wistar rats. Anatomy & Cell Biology, 45 (3), 170. doi: http://doi.org/10.5115/acb.2012.45.3.170
  15. Naeini, A. T., Miri, R., Shafiei, N., Tabandeh, M. R., Oryan, A., Nazifi, S. (2012). Effects of topical application of Calendula officinalis gel on collagen and hydroxyproline content of skin in rats. Comparative Clinical Pathology, 21 (3), 253–257. doi: http://doi.org/10.1007/s00580-010-1087-1
  16. Wang, T., Zhu, X.-K., Xue, X.-T., Wu, D.-Y. (2012). Hydrogel sheets of chitosan, honey and gelatin as burn wound dressings. Carbohydrate Polymers, 88 (1), 75–83. doi: http://doi.org/10.1016/j.carbpol.2011.11.069
  17. Butko, Y., Tkachova, O., Ulanova, V., Şahin, Y. M., Levashova, O., Tishakova. T. (2019). Immune histochemical study of KI-67 level and ribonucleic acid in the process of healing of burn wounds after treatment with drugs containing dexpanthenol and ceramide. Biointerface Research in Applied Chemistry, 9 (6), 4586–4590. doi: http://doi.org/10.33263/briac96.586590
  18. Butko, Y., Tkacheva, O., Gorbach, T. (2013). Oxidizing modification of proteins in case of a burn injury in rats against the topical treatment with new wound healing preparations. Current Issues in Pharmacy and Medical Sciences, 26 (4), 372–375. doi: http://doi.org/10.12923/j.2084-980x/26.4/a.03
  19. Tikhonov, O. I., Jarnych, T. G., Khokhlenkova, N. V. (2003). Research of ointment with phenolic hydrophobic Propolis preparation. Pharmacy in contemporary society. Kaunas, 93–97.
  20. Butko, Ia. A., Drogovoz, S. M., Deeva, T. V., Liapunov, N. A. (2013). Issledovanie reparativnykh svoistv kremov s dekspantenolom i keramidami. Nauchnye vedomosti BGU. Ser. «Meditsina. Farmatsiia», 18 (23), 176–181.
  21. Butko, Ya. O., Liapunov, M. O., Drohovoz, S. M. (2012). Vyznachennia vplyvu riznoi kontsentratsii keramidiv na protses zahoiennia shkiry. Ukrainskyi biofarmatsevtychnyi zhurnal, 5-6 (22-23), 38–40.
  22. Yakovlieva, L. V., Tkachova, O. V., Butko, Ya. O., Larianovska, Yu. B. (2013). Eksperymentalne vyvchennia novykh preparativ dlia mistsevoho likuvannia ran. Kyiv: DETs MOZ Ukrainy, 52.
  23. Belevitina, A. B., Scherbakova, S. G. (2006). Klinicheskaia interpretatsiia laboratornykh issledovanii. Saint Petersburg: ELBI-SPb, 243.

Downloads

Published

2021-04-30

How to Cite

Butko, Y., Tkachova, O., Tishakova, T., Gordienko, A. ., & Bondariev, Y. (2021). Wound healing effect of “Prolidoxid” and “Dexpanthenol with ceramides”: a сomparative study based on the model of chemical burns with expressed alterative skin processes. ScienceRise: Pharmaceutical Science, (2 (30), 26–32. https://doi.org/10.15587/2519-4852.2021.230031

Issue

Section

Pharmaceutical Science