Antioxidant and anti-cytolytic activity of parsnip (Pastinaca sativa L.) herb thick extract in conditions of catecholamine myocardiodystrophy in rats

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.253546

Keywords:

catecholamine (adrenal) myocardial dystrophy, parsnip herb thick extract (PHTE), antioxidant and anticytolytic activity

Abstract

The aim: to study the antioxidant and anticytolytic activity of the parsnip herb thick extract (PHTE) in conditions of catecholamine myocardial dystrophy in rats.

Materials and methods. Catecholamine myocardial dystrophy was reproduced in rats by a single subcutaneous injection of 0.18 % adrenaline hydrochloride solution at a dose of 0.5 mg/kg (0.28 ml/kg) of body weight. The experiment used 36 white male rats with an initial weight of 220–250 g, 4–4.5 months of age. The tested drugs were PHTE at doses of 100 and 200 mg/kg and the reference drug (RD) “Tricardin” were administered intragastrically in a therapeutic and prophylactic regimen for 7 days, the last time on the day of the experiment 1 hour before the administration of cardiotoxin. The activity of the studied agents was assessed by the survival rate of the animals, the functional state of the myocardium (ECG studies), by the effect on the heart mass ratio (HMR), the activity of aspartate aminotransferase (ASAT), the level of active products interacting with thiobarbituric acid (TBA-AP) and the content of reduced glutathione (RG) in the heart homogenate.

Results. The study of the effect of PHTE on the lipid peroxidation / antioxidant protection (LPO / AOP) system showed that a pronounced antioxidant effect of the tool was found at a dose of 200 mg/kg: there was an increase in VG at the trend level (0.05<p<0.10) and a significant decrease (p<0.05) the content of TBK-AP in 1.85 times compared with the group of positive control (PC). A similar effect was found in the comparison drug “Tricardin”. PHTE at a dose of 100 mg/kg was less influential. The use of PHTE in both doses and the comparison drug “Tricardin” reduced the severity of cytolytic processes: the activity of the enzyme AST – a marker of cardiocytolysis, was lower than in the PC group. PHTE at doses of 100 and 200 mg/kg contributed to a certain normalization of some ECG parameters compared to PC: a slight insignificant decrease in heart rate and a significant decrease (p<0.05), QT interval, systolic index (SI) and decreased ischemia marker – ST segment displacement. The comparison drug "Tricardin" showed a similar effect to PHTE on the functional state of the myocardium in adrenaline intoxication.

Conclusions. Parsnip herb thick extract exhibits antioxidant and anticytolytic activity, improves the functional state of the myocardium, prevents a decrease in the severity of alterative processes in the organ under conditions of adrenaline myocardial dystrophy

Author Biographies

Natalia Symonenko, National University of Pharmacy

Postgraduate Student

Department of Industrial Pharmacy and Economy

Institute for Advanced Training of Pharmacy Specialists

Oleh Shpychak, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Industrial Pharmacy and Economy Institute for Advanced Training of Pharmacy Specialists

Oksana Mishchenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Clinical Pharmacology

Institute for Advanced Training of Pharmacy Specialists

Viktoriia Kyslychenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Chemistry of Natural Compounds and Nutritiology

Tamara Shpychak, National University of Pharmacy

PhD, Associate Professor

Department of Organic Chemistry

Svetlana Grashchenkova, National University of Pharmacy

Senior Laboratory Assistant

Department of Clinical Pharmacology

Institute for Advanced Training of Pharmacy Specialists

References

  1. Boland, T. A., Lee, V. H., Bleck, T. P. (2015). Stress-Induced Cardiomyopathy. Critical Care Medicine, 43 (3), 686–693. doi: http://doi.org/10.1097/ccm.0000000000000851
  2. Wallner, M., Duran, J. M., Mohsin, S., Troupes, C. D., Vanhoutte, D., Borghetti, G. et. al. (2016). Acute Catecholamine Exposure Causes Reversible Myocyte Injury Without Cardiac Regeneration. Circulation Research, 119 (7), 865–879. doi: http://doi.org/10.1161/circresaha.116.308687
  3. Wang, A. R., Dean, S. A., Grebe, S. K., Hood, I. C. (2016). Fatal Catecholamine-Induced Cardiotoxicity Associated with Pheochromocytoma: Report of a Postpartum Case and Review of the Literature. Academic Forensic Pathology, 6 (2), 315–324. doi: http://doi.org/10.23907/2016.032
  4. Khara, M. R., Shkumbatiuk, O. V., Kuchyrka, L. I. (2014). Kharakterystyka stupenia poshkodzhennia miokarda adrenalinom u shchuriv riznoi stati zalezhno vid tryvalosti sposterezhennia ta rivnia statevykh hormoniv. Visnyk morfolohii, 20 (2), 372–375.
  5. Omerovic, E. (2011). How to think about stress-induced cardiomyopathy? – Think “out of the box”! Scandinavian Cardiovascular Journal, 45 (2), 67–71. doi: http://doi.org/10.3109/14017431.2011.565794
  6. Kazymyrko, V. K., Yvanytskaia, L. N., Kutovoi, V. V., Dubkova, A. H., Sylanteva, T. S. (2014). Perekysne okysnennia lipidiv: protyrichchia problemy. Ukrainskyi revmatolohichnyi zhurnal, 57 (3), 13–17.
  7. Bezkorovaina, H. O., Klishch, I. M., Khara, M. R. (2020). Gender difference of free-radical processes in the myocardium at adrenaline damage on the background of light deprivation. Medical and Clinical Chemistry, 4, 13–19. doi: http://doi.org/10.11603/mcch.2410-681x.2019.v.i4.10821
  8. Sobhani, Z., Nami, S. R., Emami, S. A., Sahebkar, A., Javadi, B. (2017). Medicinal Plants Targeting Cardiovascular Diseases in View of Avicenna. Current Pharmaceutical Design, 23 (17), 2428–2443. doi: http://doi.org/10.2174/1381612823666170215104101
  9. Zhabborova, D. M., Karomatov, I. D. (2018). Lekarstvennoe rastenie pasternak. Biologiia i integrativnaia meditsina, 22 (5), 115–120. Available at: https://cyberleninka.ru/article/n/lekarstvennoe-rastenie-pasternak
  10. Shkhanukova, Z. Kh., Konovalov, D. A. (2015). Izuchenie mineralnogo sostava pasternaka posevnogo (Pastinaca sativa L.). Sovremennye problemy nauki i obrazovaniia, 2 (2). Available at: https://science-education.ru/ru/article/view?id=23037
  11. ST-N MOZU Nastanova 42-4.0:2020 «Likarski zasoby. Nalezhna vyrobnycha praktyka» (2020). Kyiv: MOZ Ukrainy, 338. Available at: https://www.dls.gov.ua/wp-content/uploads/2020/05/Настанова-СТ-Н-МОЗУ-42-4.0_2020.pdf
  12. Kozhemiakin, Yu. M., Khromov, O. S., Filonenko, M. A., Saifetdynova H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy. Kyiv: Avitsena, 156.
  13. Liapunova, N. A., Zagoriia, V. A., Georgievskogo, V. P., Bezugloi, E. P. (Eds.) (1999). Direktiva Soveta ES o sblizhenii zakonov, postanovlenii i administrirovanie polozhenii gosudarstv ES po voprosam zaschity zhivotnykh, ispolzuemykh dlia eksperimentalnykh i drugikh nauchnykh tselei (86/609/EES). Nadlezhaschaia proizvodstvennaia praktika lekarstvennykh sredstv. Kyiv: Morion, 508–545.
  14. Stefanova, O. V. (Ed.) (2001). Doklinichni doslidzhennia likarskykh zasobiv. Kyiv: Avitsenna, 528.
  15. ST-N MOZU Nastanova 42-6.0:2008. Likarski zasoby. Nalezhna laboratorna praktyka (2012). Vydannia ofitsiine. Kyiv: Ministerstvo okhorony zdorovia Ukrainy. Available at: https://compendium.com.ua/uk/clinical-guidelines-uk/standartizatsiya-farmatsevtichnoyi-produktsiyi-tom-2/st-n-mozu-42-6-0-2008/
  16. Likarskyi zasib «Trykardyn sertsevi krapli». Derzhavnyi reiestr likarskykh zasobiv Ukrainy: Instruktsiia dlia medychnoho zastosuvannia. Available at: http://www.drlz.com.ua/ibp/ddsite.nsf/all/shlz1?opendocument&stype=4C48CB109042CC90C225863F00431A1B
  17. Fisenko, V. P. (Ed.) (2000). Rukovodstvo po eksperimentalnomu (doklinicheskomu) izucheniiu novykh farmakologicheskikh veschestv. Moscow: Remedium, 398.
  18. Shymorova, Yu. Ye., Kyslychenko, V. S. (2020). Pat. No. 139957 UA. Protyzapalnyi zasib na osnovi pasternaku posivnoho. MPK: A61K 9/08 (2006.01), A61K 36/00, A61P 29/00. No. u 2019 10054. declareted: 27.09.2019; published: 27.01.2020, Bul. No. 2.
  19. Shymorova, Yu. Ye. (2020). Pharmacognostic study of parsnip (Pastinaca sativa L.). Kharkiv, 173.
  20. Derzhavna Farmakopeia Ukrainy: in 3 edition (2015). DP «Ukrainskyi naukovyi farmatsevtychnyi tsentr yakosti likarskykh zasobiv». 2nd ed. Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmatsevtychnyi tsentr yakosti likarskykh zasobiv», 1, 1128.
  21. Horiacha, L., Symonenko, N., Galuzinska, L., Shpychak, O., Kyslychenko, V. (2020). The study of the cardioprotective action of the thick extract of parsnip herb. Ukrainian Biopharmaceutical Journal, 4 (65), 46–50. doi: http://doi.org/10.24959/ubphj.20.294
  22. Shchokina, K. H., Shtryhol, S. Yu., Bielik, H. V., Butko, Ya. O. (2010). Experimental examination of cardiotread activity of recombinant antagonist of receptors of interleukin-1 (ARYL-1) and lipin on the model of immobilization stress at rats. Ukrainskyi biofarmatsevtychnyi zhurnal, 2 (7), 39–43. Available at: http://nbuv.gov.ua/UJRN/ubfj_2010_2_9
  23. Orekhovich, V. N. (Ed.) Sovremennye metody v biohimii (1977). Moscow: Medicina, 66–68.
  24. Beutler, Е., Duron, О., Kelly, В. М. (1963). Improved method for the determination of blood glutathion. Journal of Laboratory and Clinical Medicine, 61, 882–888.
  25. Symonenko, N. A., Mishchenko, O. Ya., Shpychak, O. S., Grashchenkova, S. A., Berezniakov, A. V., Khalieieva, O. L. (2021). Cardioprotective properties of tablets with a thick extract of the herb pasternak in the conditions of adrenaline myocardiodystrophy. PharmacologyOnline, 3, 376–382. Available at: https://pharmacologyonline.silae.it/files/archives/2021/vol3/PhOL_2021_3_A041_Symonenko.pdf
  26. Iermolenko, T. I., Chorna, N. S., Shapoval, O. M. (2019). The theoretical substantiation of the search for potential medicinal products containing biologically active substances from the food plant raw material. Clinical pharmacy, 23 (2), 30–38. doi: http://doi.org/10.24959/cphj.19.1490
  27. Zaychenko, G., Mishchenko, O., Sharifov, Ch., Gordienko, A., Tkachova, О., Sinitsyna, O. et. al. (2019). Influence of extract of peach ordinary (persica vulgaris) leaves on the state of the hypothalamic-pituitary-adrenal system of rats in conditions of chronic immobilization stress. Problems of Endocrine Pathology, 68 (2), 89–97. doi: http://doi.org/10.21856/j-pep.2019.2.13
  28. Putenkova, L. Yu., Narezkina, L. P., Osipov N. M. (2010). Vliyanie adrenalina na serce lyagushki v usloviyah termicheskogo nekroza miokarda. Vestnik medicinskoj akademii, 3, 112–114.

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Published

2022-02-28

How to Cite

Symonenko, N., Shpychak, O., Mishchenko, O., Kyslychenko, V., Shpychak, T., & Grashchenkova, S. (2022). Antioxidant and anti-cytolytic activity of parsnip (Pastinaca sativa L.) herb thick extract in conditions of catecholamine myocardiodystrophy in rats. ScienceRise: Pharmaceutical Science, (1(35), 70–76. https://doi.org/10.15587/2519-4852.2022.253546

Issue

No. 1(35) (2022)

Section

Pharmaceutical Science

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Copyright (c) 2022 Natalia Symonenko, Oleh Shpychak, Oksana Mishchenko, Viktoriia Kyslychenko, Tamara Shpychak, Svetlana Grashchenkova

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