The study of carbohydrates of corn raw materials

Authors

DOI:

https://doi.org/10.15587/2519-4852.2023.274698

Keywords:

corn, corn silk, leaves, roots, carbohydrates, fractionation, gas chromatography/mass spectrometry

Abstract

The aim. The aim of the study was to study the content of polysaccharide fractions depending on the dissolution, qualitative composition and quantitative content of sugars in corn silk, leaves and roots.

Materials and methods. To study different fractions of polysaccharides - water-soluble polysaccharides (WSPS), pectin substances (PS), hemicelluloses A (HC A) and B (HM B) used a gravimetric method based on the extraction of polysaccharide fractions with a suitable solvent followed by sedimentation and weighing of the sediment. Determination of the qualitative composition and quantitative content of monosaccharides in corn medicinal plant materials (MPM) was carried out by the method of gas chromatography-mass spectrometry (GC/MS).

Results. Conducted studies of corn silk, leaves and roots carbohydrates using the fractionation method indicate the following trend of BAS accumulation in the studied raw materials: the content of WSPS and PS in corn silk exceeded the content of these compounds in other types of raw materials – 4.07±0.14 % and 7.20±0.29 %, respectively; leaves accumulated the most HC A and HC B compared to the content of these fractions in other samples - 6.81±0.21 % and 21.20±0.84 %, respectively. It was established by the GC/MS method that 4 substances were identified in corn silk in the free state: arabinose, glucose, galactose and fructose. After hydrolysis 5 substances were identified in corm silk - arabinose, xylose, mannose, glucose, galactose. Among the free sugars 3 compounds were found in corn leaves - glucose, fructose and sucrose. After hydrolysis, 4 compounds were identified in in corn leaves – arabinose, xylose, glucose and galactose. In corn roots 3 compounds are found in a free form - glucose, fructose and sucrose. After hydrolysis 4 compounds were identified in corn roots - arabinose, xylose, glucose and galactose.

Conclusions. The obtained results indicate a significant content of polysaccharides and sugars in the raw materials of corn, which makes it possible to predict the anti-inflammatory, detoxifying, adsorbing, energetic activity of the studied types of corn plant raw materials

Author Biographies

Uliana Karpiuk, Bogomolets National Medical University

Doctor of Pharmaceutical Sciences, Professor

Department of Pharmacognosy and Botany

Viktoriia Kyslychenko, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor

Department of Chemistry of Natural Compounds and Nutriciology

Ihor Hnoievyі, State Biotechnology University

Doctor of Agricultural Sciences, Professor

Department of Applied Biology and Aquatic Bioresources

Zead Helmi Abudayeh, Isra University

Doctor of Philosophy Pharmaceutical Sciences, Associate professor

Department of Applied Pharmaceutical Sciences

Andrii Fedosov, National University of Pharmacy

Doctor of Pharmaceutical Sciences, Professor, First Vice-Rector of Institution

Institution of Higher Education for Scientific and Pedagogical Work

References

  1. Mada, S. B., Sani, L., Chechet, G. D. (2020). Corn silk from waste material to potential therapeutic agent: a mini review. Fuw Trends in Science & Technology Journal, 5 (3), 816–820.
  2. Kumar D., Jhariya A. N. (2013). Nutritional, medicinal and economical importance of corn: a min review. Research Journal of Pharmaceutical Sciences, 2 (7), 1–2.
  3. Parle, M., Dhamija, I. (2013). Zea maize: a modern craze. International research journal of pharmacy, 4 (6), 39–43. https://doi.org/10.7897/2230-8407.04609
  4. Hnoievyy̆, I. V. (2006). Hodivlia i vidtvorennia poholiv’ia silskohospodarskykh tvaryn v Ukraïni. Kharliv.: OOO «Kontur», 400.
  5. Hnoievyy̆, I. V., Trishyn, O. K. (2007). Systema stalohovyrobnytstva i efektyvnoho vykorystannia kormiv za tsilorichno odnotypnoï hidovli vysokoproduktyvnykh koriv. Kharliv, 95.
  6. Gnoevoy, V. I., Hnoievyі, I. V., Trishin, A. K., Karpiuk, U. V., Kyslychenko, V. S. (2019). Content of polysaccharides in vegetative mass of maize in connection with its selection characteristic. Veterinary science, technologies of animal husbandry and nature management, 3, 29–36. doi: https://doi.org/10.31890/vttp.2019.03.05
  7. Hnoievyi, V. I., Hnoievyi, I. V., Melnyk, V. I., Bakum, M. V., Pastukhov, V. I., Lukianenko, V. M. (20171). Pat. 118211 UA. Sposib vyroouvannia zelenoï masy silskohospodarskykh kultur na korm tvarynam. MPK (2006) A01G 7/00, A01C 1/00, A01D 45/00, A23K 10/00; No u 2017 01779; declareted: 24.02.2017; published: 25.07.2017, Bul. No. 14.
  8. Derzhavna Farmakopeia Ukrainy. (2018). Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 336.
  9. Hasanudin, K., Hashim, P., Mustafa, S. (2012). Corn Silk (Stigma Maydis) in Healthcare: A Phytochemical and Pharmacological Review. Molecules, 17 (8), 9697–9715. doi: https://doi.org/10.3390/molecules17089697
  10. Suzuki, R., Iijima, M., Okada, Y., Okuyama, T. (2007). Chemical Constituents of the Style of Zea mays L. with Glycation Inhibitory Activity. Chemical and Pharmaceutical Bulletin, 55 (1), 153–155. doi: https://doi.org/10.1248/cpb.55.153
  11. Tian, S., Sun, Y., Chen, Z. (2021). Extraction of Flavonoids from Corn Silk and Biological Activities In Vitro. Journal of Food Quality, 2021, 1–9. doi: https://doi.org/10.1155/2021/7390425
  12. Oleszek, M., Kowalska, I., Oleszek, W. (2019). Phytochemicals in bioenergy crops. Phytochemistry Reviews, 18 (3), 893–927. doi: https://doi.org/10.1007/s11101-019-09639-7
  13. Ahmed, H. M. (2017). Phytochemical screening, total phenolic content and phytotoxic activity of corn (Zea mays) extracts against some indicator species. Natural Product Research, 32 (6), 714–718. doi: https://doi.org/10.1080/14786419.2017.1333992
  14. Okokon, J. E., Koofreh, D., Enin, G. (2016). Antinociceptive and anti-inflammatory activities of root extract of Zea mays. African Jornal of Pharmacology and Therapeutics, 5 (2), 111–117.
  15. Okokon, J. E., Koofreh, D., Antia, B. S. (2016). Analgesic and anti-inflammatory activities of Zea mays leaves. Journal of Herbal Drugs, 7 (2), 73–82.
  16. Mohammed, A. S. A., Naveed, M., Jost, N. (2021). Polysaccharides; Classification, Chemical Properties, and Future Perspective Applications in Fields of Pharmacology and Biological Medicine (A Review of Current Applications and Upcoming Potentialities). Journal of Polymers and the Environment, 29 (8), 2359–2371. doi: https://doi.org/10.1007/s10924-021-02052-2
  17. Hu, X., Goff, H. D. (2018). Fractionation of polysaccharides by gradient non-solvent precipitation: A review. Trends in Food Science & Technology, 81, 108–115. doi: https://doi.org/10.1016/j.tifs.2018.09.011
  18. Karpyuk, U. V., Kislichenko, V. S., Gur’eva, I. G. (2016). Carbohydrate Composition of Bryonia alba. Chemistry of Natural Compounds, 52 (4), 672–673. doi: https://doi.org/10.1007/s10600-016-1736-y
  19. Koshevoi, O. N. (2011). Amino-acid and monosaccharide compositions of Salvia officinalis leaves. Chemistry of Natural Compounds, 47 (3), 492–493. doi: https://doi.org/10.1007/s10600-011-9976-3
  20. Zhang, L., Yang, Y., Wang, Z. (2021). Extraction Optimization of Polysaccharides From Corn Silk and Their Antioxidant Activities in vitro and in vivo. Frontiers in Pharmacology, 12. doi: https://doi.org/10.3389/fphar.2021.738150
  21. Horpinchenko, І. І., Spiridonenko, V. V. (2018). Study of the effectiveness of D-mannose in women with recurrent cystitis on the background of diabetes mellitus. Medical aspects of man's health, 4 (31), 28–35.
  22. Scaglione, F., Musazzi, U. M., Minghetti, P. (2021). Considerations on D-mannose Mechanism of Action and Consequent Classification of Marketed Healthcare Products. Frontiers in Pharmacology, 12. 636377. https://doi.org/10.3389/fphar.2021.636377
  23. Abedon, B. G., Hatfield, R. D., Tracy, W. F. (2006). Cell Wall Composition in Juvenile and Adult Leaves of Maize (Zea mays L.). Journal of Agricultural and Food Chemistry, 54 (11), 3896–3900. https://doi.org/10.1021/jf052872w
  24. Couture, G., Luthria, D. L., Chen, Y., Bacalzo, N. P., Tareq, F. S., Harnly, J. et al. (2022). Multi-Glycomic Characterization of Fiber from AOAC Methods Defines the Carbohydrate Structures. Journal of Agricultural and Food Chemistry, 70 (45), 14559–14570. doi: https://doi.org/10.1021/acs.jafc.2c06191
  25. Kurzyna-Szklarek, M., Cybulska, J., Zdunek, A. (2022). Analysis of the chemical composition of natural carbohydrates – An overview of methods. Food Chemistry, 394, 133466. https://doi.org/10.1016/j.foodchem.2022.133466

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Published

2023-02-28

How to Cite

Karpiuk, U., Kyslychenko, V., Hnoievyі I., Buhay, T., Abudayeh, Z. H., & Fedosov, A. (2023). The study of carbohydrates of corn raw materials. ScienceRise: Pharmaceutical Science, (1(41), 32–40. https://doi.org/10.15587/2519-4852.2023.274698

Issue

No. 1(41) (2023)

Section

Pharmaceutical Science

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Copyright (c) 2023 Uliana Karpiuk, Viktoriia Kyslychenko, Ihor Hnoievyі, Tatiana Buhay, Zead Helmi Abudayeh, Andrii Fedosov

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