Morphological parameters of maize endospermal mutants seedlings and effect of seed storage regimes on them

Authors

  • T. P. Shyianova Plant Production Institute nd. a V.Ya. Yuriev of NAAS, Ukraine
  • S. M. Tymchuk Plant Production Institute nd. a V.Ya. Yuriev of NAAS, Ukraine
  • R. L. Boguslavskyi Plant Production Institute nd. a V.Ya. Yuriev of NAAS, Ukraine

DOI:

https://doi.org/10.30835/2413-7510.2018.134376

Keywords:

maize, seeds, seedlings, morphology, endospermal mutants, longevity, accelerated aging, freezing.

Abstract

Endospermal mutants of maize are a source of valuable raw materials for various industries and directions of consumption. Therefore, problem of their seeds longevity is relevant for storage in genebanks, breeding and seed-growing establishments.

The aim and tasks of the study was to determine effect of different storage regimes on morphometric characteristics of seedlings from seeds of maize endosperm mutants.

Material and methods. Seeds of six simple hybrids F1 and 12 their parental lines – carriers of endospermal mutations: wx, ae, su1, se, sh2, and dentate type are used as the material. The seeds were affected by accelerated aging using the method of B.S. Likhachev (1978), freezing during a month and combined regime – accelerated aging + freezing.

Results and discussion. The decrease in the indices of all morphometric traits under influence of three factors was shown by carriers of the mutation sh2 ♀SS386 and ♂SS389. Their F1 hybrid, as a rule, increased the indices and reduced only length of the seedling under the combined regime. Reduction of most indicators of morphometric traits under accelerated aging was observed in carriers wx: Binom, ♀VK69 and ♂VK64. Positive effect of accelerated aging and freezing was observed on the indices of all three morphological characters in ae carriers: AE800/AE392, ♀AE392, ♂ AE800. The excess of the hybrid over the parent lines in length of seedling and number of roots after accelerated aging and freezing manifested in the dentate hybrid Vympel, in the number and total length of roots – in carriers of sh2 SS389 / SS386; in total roots length and seedling length – from the carrier su1 Dmytryk. In general, the hybrids AE800/AE392 (ae) and SS389/SS386 (sh2) are more homeostatic than the parent lines.

Conclusions. The highest longevity is characterized for the seeds of dentate maize and carriers of ae and su1, comparatively low longevity–carriers of wx and se, and the lowest – carriers of sh2.

References

Smith AM, Martin C. Starch biosynthesis and the potential for its manipulation. Biosynthesis and manipulation of plant products. Plant biotechnology. In: D Grierson, editor. Glasgow: Blackie and Son Publ., 1993. Vol. 3. P. 1–54.

Hannah LC, Giroux M, Boyer CD. Biotechnological modification for sweet corn and maize improvement. Sci. Hortic. 1993; 55: 177–197.

Ferguson VL. High amylose and waxy corns. Specialty corns. In: AR Hallauer, editor. Boca Raton, Fl.: CRC Press, 1994. P. 55–779.

Nelson OE, Pan D. Starch synthesis in maize endosperm. Ann. Rev. Plant Physiol. Plant Mol. Biol. 1995; 46: 475–496.

Tracy WF. History, breeding, and genetics of super-sweet corn. Plant Breeding Review. 1997; 14: 189–236.

Linnyk YuO, Tymchuk SM, Chupikov MM, Kuzmishina NV. Tolerance of seeds of different corn subspecies to aging. Tavriyskyi naukovyi visnyk. 2010; 69: 67–74.

Wilson JrDO, Mohan SK, Ratcliff SL, Knapp AD. Effect of harvest and condition in gon vigor of shrunken 2 sweet corn seed. J. Prod. Agric.1994; 7: 335–339.

Parera CA, Cantliffe DJ, McCarty DR, Hannah LC. Improving vigor in shrunken-2 corn seedlings. J. Amer. Soc. Hort. Sci. 1996; 121: 1069–1075.

Douglass SC, Juvik JA. Cold soil emergence and variation in sweet corn kernel carbohydrate reserves. Hort. Sci. 1987; 22: 1097–1102.

Rowe DE, Garwood DL. Effects of four maize endosperm mutants on kernel vigor. Crop Science. 1978; 18: 709–712.

Yousef GG, Juvik JA. Enhancement of seedling emergence in sweet corn by marker–assisted backcrossing of beneficial QTL. Crop Sci. 2002; 42: 96–104.

Revilla P, Velasco P, Malvar RA et al. Variability among maize inbredlinesforseed longevity. Genet. Res. And Crop Evol. 2006; 53: 771–777.

Woltz J, Tekrony D. Accelerated aging test for corn seed. Seed Technol. 2001; 23:21–34.

Miloševic M, Vujakovic M, Karagic D. Vigour tests as indicators of seed viability. Genetika. 2010; 42(1): 103–118.

Likhachev BS. Some methodological issues of research into seed aging biology. Selskokhoziaystvennaia biologiia. 1980; XV(6): 842–844.

Standards of gene banks of plant genetic resources for food production and agriculture. FAO, 2015, 180 p.

Linnyk YuO. Influence of negative temperature on viability indices of seeds. Visnyk Poltavskoyi derzhavnoyi agrarnoyi akademiyi. 2010; 3(58): 175–179.

Douglass SC, Juvik JA. Cold soil emergence and variation in sweet corn kernel carbohydratereserves. Hort. Sci. 1987; 22: 1097–1102.

Shyianova TP, Tymchuk SM, Boguslavskyi RL. Longevity of corn seeds with different structure of endosperm. Genetychni resursy Roslyn. 2015; 17: 87–98.

Euro-standard. Corn seeds. FAO Classification of corn hybrids.URL: http://eurostd.com/?p=97.

Seeds of agricultural crops. Methods for determining quality. DSTU 4138-2002. 2004-01-01. Kyiv: Derzhspozhyvstandart Ukrayiny, 2003. 173 p.

Likhachev BS. Morphological evaluation of seedlings and germination energy of seeds. Selektsiia I semenovodstvo. 1977; 3: 67–68.

Kakin GF. Biometrics. Мoscow: Vysshaia shkola, 1990. 350 p.

Diagnosis of plant resistance to stress factors. Leningrad: VIR, 1988. 228 p.

Gustafson A. The effect of heterozygosity on variability and vigour. Hereditas. 1946; 32: 263–284.

Downloads

Published

2018-07-30

Issue

Section

PHYSIOLOGICAL, GENETICAL AND BIOCHEMICAL STUDIES