Correlation between valuable economic features and quality indicators in sunflower lines with altered composition of tocopherol isomers
DOI:
https://doi.org/10.30835/2413-7510.2019.190457Keywords:
sunflower, tocopherol isomers, fatty acid composition, protein content, oil content, performance, 1000-seed weight, correlationAbstract
The purpose of our study was to determine the contents of tocopherol isomers and to analyze correlations between valuable economic features and quality indicators in sunflower lines - pollen fertility restorers with altered composition of tocopherol isomers.
Materials and methods. The study was conducted at the Plant Production Institute named after V.Ya. Yuriev of NAAS in 2017-2018. Ten lines - pollen fertility restores with altered tocopherol isomer composition were taken as the test material.
Tocopherol isomer contents were determined by high performance liquid chromatography. The oil content in sunflower seeds was determined by weighing after oil extraction using a Soxhlet extractor. The protein content was determined by the Kjeldahl titrimetric method. The fatty acid composition of oil was determined on a gas chromatograph Selichrom 1 using a modification of the Peisker method.
Results and discussion. In 2017-2018 at the Plant Production Institute named after V.Ya. Yuriev 10 lines - pollen fertility restorers with altered tocopherol composition and other valuable economic features were developed through self-pollination and individual selections. Lines Kh1717V, Kh1716V, Kh1747V, Kh1729V, and Kh1712V have an increased β-tocopherol content: 24.50 mg%, 19.87 mg%, 15.67 mg%, 15.09 mg%, and 12.19 mg%, respectively. A high content of γ-tocopherol was found in lines Kh1719V, Kh1738V, Kh177V, and Kh1711V: 35.70 mg%, 14.73 mg%, 8.47 mg%, and 7.80 mg%, respectively. An increased content of δ-tocopherol was observed in lines Kh1729V (7.08 mg%), Kh1712V (5.85 mg%), and Kh1719V (3.64 mg%).
In our tests, the correlation coefficients between the tocopherol composition and the contents of oil, protein, oleic and linoleic acids were calculated. There was no significant correlations between these parameters, while there was a significant negative correlation between the β-tocopherol content and 1000-seed weight.
Conclusions. The new lines with altered composition of tocopherol isomers have been developed; they are adapted to the growing conditions of the eastern forest-steppe of Ukraine, which allows a new direction in sunflower breeding for quality. Creation of hybrids with oxidation-resistant oil is of great importance for the development of our country's agricultural sector.
Analysis of the correlations between different valuable economic features showed that, when creating new breeding material, one should pay attention the content of β-tocopherol and 1000-seed weight, since there was a significant negative correlation between these two traits.
References
Kyrychenko VV, Petrenkova VP, Syvenko VI, Makliak KM et al. The strategy of production of oil raw materials in Ukraine until 2020: guidelines. Kharkiv, 2016. 142p.
Zajtsev OM. Introduction of new sunflower hybrids is a way to increase the profitability of agricultural production. Propozytsiia. 2002; 8: 22–29.
Bugajov VD, Vasylkivskyi SP, Vlasenko VA. Special breeding of field crops. Manual. MYa Molotskyi, ed. Bila Tserkva, 2010. 368 p.
Borodulina AA, Popov PS, Snesar EV. Changing the oil quality in different sunflower varieties during storage of seeds. Nauchno-tekhnicheskii biuleten VNIIMK. 1985; 87: 25–27.
Kyrychenko VV, Tymchuk SM, Bragin OM. Genetic diversity of sunflower lines by fatty acid composition of oil. Henetychni resursy Roslyn. 2007; 4: 131–139.
Pelekhova LS, Usatiuk SI. Evaluation of the total antioxidant activity of vegetable oils. Naukovi pratsi Natsionalnogo universytetu kharchovykh tekhnologiy. 2012; 44: 81–84.
Nadirov NK. Tocopherols and their use in medicine and agriculture. Moscow: Nauka, 1991. 335 p.
Burlakova EB. Bioantioxidants. Rossiyskiy khimicheskiy zhurnal.2007; 51(1): 3–12.
Ivanov II, Merzliak MN, Tarusov Vitamin E, its biological role in relation to antioxidant properties. Collection Bioantioxidants. Proceedings of Moscow Society of Naturalists. Moscow: Nauka, 1975. P. 30–52.
Smirnov VA, Klimochkin YuN. Vitamins and coenzymes: manual. Part 2. Samara: Samarskiy gosudarstvennyi tekhnicheskiy universitet, 2008. 91 p.
Demurin YaN. Genetic analysis and breeding use of fatty acid and tocopherol composition traits in sunflower seeds. [dissertation]. Sankt-Peterburg, 1999.
Demurin YaN. Genetic study of quality parameters of sunflower oil at the All-Russian Research Institute of Oil Crops. The main results of research on oil crops (to the 100th anniversary of the All-Russian Research Institute of Oil Crops). Krasnodar, 2012.P. 113–121.
Demurin YaN. Genetic analysis of the tocopherol composition in sunflower seeds. Nauchno-tekhnicheskiy biulleten VIR. 1986; 165: 49–51.
Demurin YаN, Škorić D, Karlović DJ. Genetic variability of tocopherol composition in sunflower seeds as a basis of breeding for improved oil quality. Plant Breeding.1996; 115: 33–56.
Determination of vitamin E content by high resolution liquid chromatography of α-, β-, γ-, δ-tocopherols (EN 12822: 2000, IDT): State Standard of Ukraine EN 12822: 2005. [Valid from 2006-07-01]. (National Standard of Ukraine). Kyiv: DerzhspozhyvstandartUkrayiny, 2006. 13 p.
Methods of state scientific and technical examination of plant varieties. Methods for determination of quality parameters of plant products. Tkachyk SO, ed. 4th revised and expanded edition. Vinnytsia: TOV «Nilan-LTD», 2015. 160 p.
Methods of biochemical studies of plants. AI Ermakov, ed. Изд. 3rd revised and expanded edition. Leningrad: Agropromizdat, 1987. 432 p.
Prokhorova MI. Methods of biochemical studies. Leningrad: Khimiia, 1982. 272 p.
Dospekhov BA. Methods of field experience. Moscow: Agropromizdat, 1985. 351 p.
Downloads
Published
Issue
Section
License
Copyright (c) 2019 Н. С. Харитоненко, В. П. Коломацька, В. В. Кириченко, О. В. Анциферова, Т. А. Шелякіна, О. Г. Супрун, В. С. Лютенко
This work is licensed under a Creative Commons Attribution 4.0 International License.
When placing the text in electronic resources, the copyright is reserved by the author of a publication.
Authors may not agree with referee’s(s’) comments and remarks of the Editorial Board, rationalizing their point of view.
Authors may require clarification from or the Editorial Board or changes in the event of significant errors in their article.
Authors can use materials published in the journal Selection and Seed Industry in their work, mandatorily referring to our journal.
