How to escape 'the eskape pathogens' using plant extracts

Authors

DOI:

https://doi.org/10.15587/2519-8025.2019.193155

Keywords:

isolates of clinical origin, biofilms, plant extracts

Abstract

The aim of the work was to determine the content of biologically active substances, namely polyphenols and anthocyanins, in the extracts of cherryplum, blueberries, jostaberry, sweet cherries, plums, red and black currants, and to study the effect of these extracts on the growth and biofilm formation of clinical isolates of ESKAPE Pathogens in vitro.

Materials and methods. The antibiotic resistance of the following clinical isolates: Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae was determined by the Kirby-Bauer diffusion method. The bioactive substances content was determined with a thin layer chromatography method. The effect of berry extracts on the above-mentioned isolates was studied by the method of compatible cultivation. The capacity of clinical isolates to form a biofilm was studied by a spectrophotometric method, using gentian violet.

Results. Having analyzed the results of the antibiotic sensitivity of clinical isolates, it was found, that they were resistant to all antimicrobials used. The analysis of the content of bioactive substances of berry extracts showed that they contain a large amount of anthocyanins and polyphenols. It was revealed, that clinical isolates are capable of forming biofilms, and the selected berry extracts had the ability to inhibit the formed biofilms with isolates of clinical origin, such as Klebsiella pneumoniae and Pseudomonas aeruginosa.

Conclusions. Researching the antibacterial properties of anthocyanins and polyphenols, extracted from berries, we can conclude, that they are capable of inhibiting the growth of not only planktonic strains forms, selected by us, but also inhibit the biofilms, formed by them

Author Biographies

Oleksandra Pallah, State Higher Educational Institution «Uzhgorod National University» Narodna sq., 3, Uzhhorod, Ukraine, 88000

Assistant, Junior Researcher

Department of Clinical and Laboratory Diagnosis and Pharmacology

Molecular Microbiology and Immunology Center for Molecular Microbiology and Immunology

Tamara Meleshko, Senior Lecturer, Junior Researcher Department of Clinical and Laboratory Diagnosis and Pharmacology Molecular Microbiology and Immunology Center for Molecular Microbiology and Immunology

State Higher Educational Institution «Uzhgorod National University»

Narodna sq., 3, Uzhhorod, Ukraine, 88000

Svitlana Tymoshchuk, State Higher Educational Institution «Uzhgorod National University» Narodna sq., 3, Uzhhorod, Ukraine, 88000

Assistant, Junior Researcher

Department of Clinical and Laboratory Diagnosis and Pharmacology

Molecular Microbiology and Immunology Center for Molecular Microbiology and Immunology

Lesya Yusko, State Higher Educational Institution «Uzhgorod National University» Narodna sq., 3, Uzhhorod, Ukraine, 88000

PhD, Lecturel, Junior Researcher

Department of Clinical and Laboratory Diagnosis and Pharmacology

Molecular Microbiology and Immunology Center for Molecular Microbiology and Immunology

Larisa Bugyna, State Higher Educational Institution «Uzhgorod National University» Narodna sq., 3, Uzhhorod, Ukraine, 88000

Researcher

Molecular Microbiology and Immunology Center for Molecular Microbiology and Immunology

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Published

2020-01-29

How to Cite

Pallah, O., Meleshko, T., Tymoshchuk, S., Yusko, L., & Bugyna, L. (2020). How to escape ’the eskape pathogens’ using plant extracts. ScienceRise: Biological Science, (5-6(20-21), 30–37. https://doi.org/10.15587/2519-8025.2019.193155

Issue

No. 5-6(20-21) (2019)

Section

Biological Sciences

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Copyright (c) 2020 Oleksandra Pallah, Tamara Meleshko, Tamara Meleshko, Svitlana Tymoshchuk, Svitlana Tymoshchuk, Lesya Yusko, Lesya Yusko, Larisa Bugyna, Larisa Bugyna

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