Identification of SRC, LCK, ABL, JAK1 genes exrression in lymphohemopoietic complex cells on fetal material application

Authors

  • Анатолій Миколайович Гольцев Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016, Ukraine https://orcid.org/0000-0002-9574-2407
  • Олена Дмитрівна Луценко Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016, Ukraine https://orcid.org/0000-0001-9765-5473
  • Катерина Євгенівна Ямпольська Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016, Ukraine https://orcid.org/0000-0002-5138-3385
  • Максим Вадимович Останков Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016, Ukraine https://orcid.org/0000-0001-9930-305X
  • Миколай Олександрович Бондарович Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016, Ukraine https://orcid.org/0000-0003-1004-3947

DOI:

https://doi.org/10.15587/2519-8025.2016.72757

Keywords:

lymphohemopoietic complex, proto-oncogene expression, fetal liver cell, cell therapy

Abstract

The application of fetal cells in clinical practice determines the necessity of solving the tasks related to their safety. Тhere was investigated the expression rate of genes src, abl, lck, jak1 in cells of lymphohemopoietic complex of СВА/H and С3Н/Не mice before and after application of fetal liver cells. After application of fetal cells no significant changes in gene expression in cells of both mice lines were revealed

Author Biographies

Анатолій Миколайович Гольцев, Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016

Academician of the National Academy of Sciences of Ukraine
Director Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine

MD, professor 

Олена Дмитрівна Луценко, Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016

PHD

Department of cryopathophysiologi and immunology

Катерина Євгенівна Ямпольська, Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016

PHD

Department of cryopathophysiologi and immunology

Максим Вадимович Останков, Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016

PHD

Department of cryopathophysiologi and immunology

Миколай Олександрович Бондарович, Institute for Problems of Cryobiology and Cryomedicine Pereyaslavskaya str., 23, Kharkov, Ukraine, 61016

PHD

Department of cryopathophysiologi and immunology

References

  1. Grishchenko, V. I., Goltsev, A. N. (2002). Transplantacija produktiv embriofetoplacentarnogo kompleksa. Ot ponimanija mehanisma deystvija k povisheniu effektivnosti primenenija [Transplantation of products of embriofetoplacental complex. From understanding of action mechanism to the effectiveness of application]. Problems of Cryobiology, 2, 54–84.
  2. Goltsev, A. M., Dubrava, T. G., Lutsenko, O. D., Porozhan, E. O., Babenko, N. M., Gayevska, Y. O., Dimitrov, O. Y. (2013). Molekuljarni mehanismi imunokoreguval’noi dii preparative fetoplacentarnogo kompleksy pri rozvitku autoimunih zahvorjuvan’ [Molecular mechanisms of immunocorrectional action of drugs of fetoplacental development of autoimmune diseases]. Odessa med. Magazine, 138 (4), 13–18.
  3. Gorczynski, R. M., Alexander, C., Bessler, W., Brandenburg, K., Fournier, K., Mach, J. P. et. al. (2007). An alteration in the levels of populations of CD4+ Treg is in part responsible for altered cytokine production by cells of aged mice which follows injection with a fetal liver extract. Immunology Letters, 109 (2), 101–112. doi: 10.1016/j.imlet.2007.01.009
  4. Gordeeva, O. F., Nikonova, T. M. (2013). Development of Experimental Tumors Formed by Mouse and Human Embryonic Stem and Teratocarcinoma Cells After Subcutaneous and Intraperitoneal Transplantations Into Immunodeficient and Immunocompetent Mice. Cell Transplant, 22 (10), 1901–1914. doi: 10.3727/096368912x657837
  5. Croce, C. M. (2008). Oncogenes and Cancer. New England Journal of Medicine, 358 (5), 502–511. doi: 10.1056/nejmra072367
  6. Bruno, R. D., Rosenfield, S. M., Smith, G. H. (2013). Late developing mammary tumors and hyperplasia induced by a low-oncogenic variant of mouse mammary tumor virus (MMTV) express genes identical to those induced by canonical MMTV. Molecular Cancer, 12 (1), 79. doi: 10.1186/1476-4598-12-79
  7. Goltsev, A. M., Bondarovych, M. O., Kuznyakov, A. V., Ostankova, M. V., Ostankova, L. V., Chelombitko, O. V. (2014). Viznachennja stanu T-klitinnoi lanki imunitetu i vmistu stovburovih rakovih klitin jak kriterij ocinki efektivnosti preventivnoji terapiiraku molochnoi gelezi kriokonservovanimi klitinami fetal’noi pechinki [Defining the T-cell immunity and content of stem cancer cells as a criterion for evaluating the effectiveness of preventive treatment of breast cancer cells cryopreserved fetal liver]. Problems of Cryobiology and Cryomedicine, 24 (3), 238–248.
  8. Holt, M. P., Shevach, E. M., Punkosdy, G. A. (2013). Endogenous Mouse Mammary Tumor Viruses (Mtv): New Roles for an Old Virus in Cancer, Infection, and Immunity. Frontiers in Oncology, 3. doi: 10.3389/fonc.2013.00287
  9. Blandova, Z. K., Dushkin, V. A., Malashenko, A. M., Schmidt, E. F. (1983). Linii laboratornih zivotnih dlja medico-biologicheskih issledovaniy[Lines of laboratory animals for medical and biological research]. Moscow: Science, 192.
  10. Herrington, S., Makgi, Dzh.; Herringtona, S. (Ed.) (1999). Molecularnaja klinicheskaja diagnostika. Methodi [Molecular clinical diagnostics. Methods]. Moscow: Mir, 307–309.
  11. Trofimov, D. Y., Burmenskaya, O. V., Donetskova, A. D. et. al. (2008). Razrabotka test system dlja opredelenija urovnja expressii mRNKFOXP3 na osnove OT-PCR v regime real’nogo vremeny [Development of test systems for the determination of mRNA FOXP3 expression levels through RT-PCR in real time]. Immunology, 29 (3), 182–187.
  12. Chaudhari, S., Desai, J. S., Adam, A., Mishra, P. (2014). JAK/STAT as a novel target for treatment of leukemia. Int. J. of Pharmacy and Pharmaceutical Sciences, 6 (1), 1–7.
  13. Summy, J. M., Gallick, G. E. (2003). Src family kinases in tumor progression and metastasis. Cancer Metastasis Rev, 22 (4), 337–358.
  14. Neron, S. (2005). B cell proliferation following CD40 stimulation results in the expression and activation of Src protein tyrosine kinase. International Immunology, 18 (2), 375–387. doi: 10.1093/intimm/dxh377
  15. Lopez, P. F., Parks, W. P., Lopez, D. M. (1984). Antigenic determinants in a virus-induced mouse mammary tumor recognized by cell-mediated immune assays. J. Natl. Cancer. Inst, 72 (3), 725–732
  16. Filipp, D., Zhang, J., Leung, B. L., Shaw, A., Levin, S. D., Veillette, A., Julius, M. (2003). Regulation of Fyn Through Translocation of Activated Lck into Lipid Rafts. The Journal of Experimental Medicine, 197 (9), 1221–1227. doi: 10.1084/jem.20022112
  17. Ariyoshi, K., Takabatake, T., Shinagawa, M., Kadono, K., Daino, K., Imaoka, T. et. al. (2014). Age Dependence of Hematopoietic Progenitor Survival and Chemokine Family Gene Induction after Gamma Irradiation in Bone Marrow Tissue in C3H/He Mice. Radiation Research, 181 (3), 302–313. doi: 10.1667/rr13466
  18. Khaldoyanidi, S., Denzel, A., Zoller, M. (1996). Requirement for CD44 in proliferation and homing of hematopoietic precursor cells. Journal of Leukocyte Biology, 60 (5), 579–592.
  19. Liu, Y., Yue, W., Ji, L., Nan, X., Pei, X. (2010). Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment. BMC Developmental Biology, 10 (1), 85. doi: 10.1186/1471-213x-10-85
  20. Lee, K. Y., Fong, B. S. P., Tsang, K. S., Lau, T. K., Ng, P. C., Lam, A. C. et. al. (2011). Fetal Stromal Niches Enhance Human Embryonic Stem Cell–Derived Hematopoietic Differentiation and Globin Switch. Stem Cells and Development, 20 (1), 31–38. doi: 10.1089/scd.2010.0196
  21. Takahashi, T., Inada, S., Pommier, C. G., O’Shea, J. J., Brown, E. J. (1985). Osmotic stress and the freeze-thaw cycle cause shedding of Fc and C3b receptors by human polymorphonuclear leukocytes. J. Immunol, 134 (6), 4062–4068.
  22. Korzhov, V. I., Korzhov, M. V., Punschikova, E. A., Sakhnenko, A. S. (2008). Belki teplovogo shoka [ Heat shock proteins]. J. of AMS of Ukrainе, 14 (1), 26–42.
  23. Borysov, P., Dimitrov, A., Ostankov, M., Babenko, N. (2014). The Effect of Cryopreservation on the Expression of Stemness-Genes in Mouse Fetal Liver Cells. Refrigeration Science and Technology, 1, 207–211.

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Published

2016-07-01

How to Cite

Гольцев, А. М., Луценко, О. Д., Ямпольська, К. Є., Останков, М. В., & Бондарович, М. О. (2016). Identification of SRC, LCK, ABL, JAK1 genes exrression in lymphohemopoietic complex cells on fetal material application. ScienceRise: Biological Science, (1 (1), 40–46. https://doi.org/10.15587/2519-8025.2016.72757

Issue

No. 1 (1) (2016)

Section

Biological Sciences

License

Copyright (c) 2016 Анатолій Миколайович Гольцев, Олена Дмитрівна Луценко, Катерина Євгенівна Ямпольська, Максим Вадимович Останков, Миколай Олександрович Бондарович

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