Screening of laccase producing fungi

Authors

  • Ольга Михайлівна Демків Institute of Cell Biology of NAS Ukraine Drahomanov str., 14/16, Lviv, Ukraine, 79005, Ukraine https://orcid.org/0000-0002-7999-4436
  • Соломія Ярославівна Банах Ivan Franko national university of Lviv Hrushvskogo stt., 4, Lviv, Ukraine, 79005, Ukraine
  • Сергій Вікторович Притула Ivan Franko national university of Lviv Hrushvskogo str., 4, Lviv, Ukraine, 79005, Ukraine
  • Галина Зуфарівна Гайда Institute of Cell Biology of NAS Ukraine, Drahomanov str., 14/16, Lviv, Ukraine, 79005, Ukraine https://orcid.org/0000-0003-4015-8083
  • Михайло Васильович Гончар Institute of Cell Biology NAS of Ukraine, Drahomanov str., 14/16, Lviv, Ukraine, 79005 Rzeszow University Sokolowska str., 26, Kolbuszowa, Poland, 36-100, Ukraine https://orcid.org/0000-0001-8861-4477

DOI:

https://doi.org/10.15587/2519-8025.2016.83698

Keywords:

fungi, screening of producing strains, laccase, Stachybotris chartarum strain, cultivation conditions

Abstract

The screening of fungal strains on a capability to laccase synthesis was carried out and the best producers of extracellular enzyme – Stachybotris chartarum and Monilinia fructicola strains were selected. The ability of the fungus S. chartarum to produce laccase extracellularly was demonstrated in the first time, the optimal conditions for cultivation of the cells were selected, analytical quantity of a goal enzyme was obtained and the molecular mass of laccase subunit was determined (50 кDa)

Author Biographies

Ольга Михайлівна Демків, Institute of Cell Biology of NAS Ukraine Drahomanov str., 14/16, Lviv, Ukraine, 79005

PhD

Department of analytical biotechnology, junior scientist

Галина Зуфарівна Гайда, Institute of Cell Biology of NAS Ukraine, Drahomanov str., 14/16, Lviv, Ukraine, 79005

PhD, senior reseacher

Department of analytical biotechnology, senior scientist

Михайло Васильович Гончар, Institute of Cell Biology NAS of Ukraine, Drahomanov str., 14/16, Lviv, Ukraine, 79005 Rzeszow University Sokolowska str., 26, Kolbuszowa, Poland, 36-100

Doctor of Biological Science, Professor, Head of the Department

Department of Analytical Biotechnology

scientific consultant

Institute of Applied Biotechnology and Basic Sciences,

References

  1. Piscitelli, A., Pezzella, C., Lettera, V., Giardina, P., Faraco, V., Sannia, G. (2013). Fungal Laccases. Fungal Enzymes, 113–151. doi: 10.1201/b15247-6
  2. Levine, W. G. (1965). Laccase, a review. In: The biochemistry of copper New York: Academic Press Inc., 371–385.
  3. Kües, U. (2015). Fungal enzymes for environmental management. Current Opinion in Biotechnology, 33, 268–278. doi: 10.1016/j.copbio.2015.03.006
  4. Brijwani, K., Rigdon, A., Vadlani, P. V. (2010). Fungal Laccases: Production, Function, and Applications in Food Processing. Enzyme Research, 2010, 1–10. doi: 10.4061/2010/149748
  5. Viswanath, B., Rajesh, B., Janardhan, A., Kumar, A. P., Narasimha, G. (2014). Fungal Laccases and Their Applications in Bioremediation. Enzyme Research, 2014, 1–21. doi: 10.1155/2014/163242
  6. Rodríguez Couto, S., Toca Herrera, J. L. (2006). Industrial and biotechnological applications of laccases: A review. Biotechnology Advances, 24 (5), 500–513. doi: 10.1016/j.biotechadv.2006.04.003
  7. Moin, S., Omar, M. (2013). Laccase Enzymes: Purification, Structure to Catalysis and Tailoring. PPL, 21 (8), 707–713. doi: 10.2174/09298665113209990058
  8. Madhavi, V., Lele, S. S. Laccase: properties and applications. (2009). BioResources, 4 (4), 1694–1717. Available at: http://ncsu.edu/bioresources
  9. Shleev, S. V., Morozova, O. V., Nikitina, O. V., Gorshina, E. S., Rusinova, T. V., Serezhenkov, V. A. et. al. (2004). Comparison of physico-chemical characteristics of four laccases from different basidiomycetes. Biochimie, 86 (9-10), 693–703. doi: 10.1016/j.biochi.2004.08.005
  10. Valle, J. S., Vandenberghe, L. P. S., Santana, T. T., Almeida, P. H., Pereira, A. M., Linde, G. A. et. al. (2014). Optimum conditions for inducing laccase production in Lentinus crinitus. Genetics and Molecular Research, 13 (4), 8544–8551. doi: 10.4238/2014.october.20.31
  11. Khan, M., Motiar, R., Ray, M., Ray, L., Guha, A. K. (2016). Extracellular laccase from Pleurotus sajor-caju: Fermentative conditions and influence of nitrogenous sources. Indian Journal of Biotechnology, 15, 230–235.
  12. El-Fakharany, E. M., Hassan, M. A., Taha, T. H. (2016). Production and Applicationof Extracellular Laccase Produced by Fusarium oxysporum EMT. International Journal of Agriculture and Biology, 18 (05), 939–947. doi: 10.17957/ijab/15.0190

Downloads

Published

2016-11-18

How to Cite

Демків, О. М., Банах, С. Я., Притула, С. В., Гайда, Г. З., & Гончар, М. В. (2016). Screening of laccase producing fungi. ScienceRise: Biological Science, (3 (3), 64–69. https://doi.org/10.15587/2519-8025.2016.83698

Issue

No. 3 (3) (2016)

Section

Biological Sciences

License

Copyright (c) 2016 Ольга Михайлівна Демків, Соломія Ярославівна Банах, Сергій Вікторович Притула, Галина Зуфарівна Гайда, Михайло Васильович Гончар

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.

Authors, who are published in this journal, agree to the following conditions:

1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.

 2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.